Cryogenic stabilization of myoglobin photoproducts

M. Sassaroli, S. Dasgupta, D. L. Rousseau

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Abstract

The low frequency resonance Raman spectra of photodissociated carbon monoxymyoglobin at cryogenic temperatures (4-77 K) differ from those of deoxymyoglobin. Intensity differences occur in several low frequency porphyrin modes, and intensity and frequency differences occur in the iron-histidine stretching mode. This mode appears at about 225 cm-1 in deoxymyoglobin. At the lowest temperature studied, ~4 K, the frequency of the iron-histine stretching mode in the photoproduct is ~223 cm-1, and the intensity is very low. When the temperature of the photoproduct is increased, the intensity of the mode increases, but its frequency is unchanged. The differences between the photoproduct and the deoxy preparation persist to 77 K, the highest temperature studied, and are independent of whether samples are frozen in phosphate buffer or a 50:50 ethylene glycol/phosphate buffer mixture. It is proposed that the frequency of the iron-histidine stretching mode is governed by the tilt angle of the histidine with respect to the normal to the heme plane, and the intensity of the mode is governed by the overlap between the δ* orbital of the iron-histidine bond and the π* orbital of the porphyrin macrocycle. This model can account for differences between the resonance Raman spectra for the photoproduct and the deoxy preparations of both hemoglobin and myoglobin. Furthermore, by considering the F-helix motions in going from 6-coordinate to 5-coordinate hemoglobin and myoglobin, the heme relaxation of these proteins at room temperature with 10-ns pulses can be explained. Based on the findings reported here, low temperature relaxation pathways for both hemoglobin and myoglobin are proposed.

Original languageEnglish (US)
Pages (from-to)13704-13713
Number of pages10
JournalJournal of Biological Chemistry
Volume261
Issue number29
Publication statusPublished - Dec 1 1986
Externally publishedYes

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ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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