A wide variety of bifunctional protein reagents will covalently cross-link the elongation factor EF-G to the 50S ribosomal subunit of Escherichia coli. About 120,000 daltons of ribosomal proteins become cross-linked to EF-G upon extensive reaction. About 30,000 daltons of this material is made up of proteins L7 and L12 (mol wt 13,000 daltons). Thus, these two components, whose presence on the ribosome is required for the EF-G interaction, must be located at the EF-G binding site.
|Original language||English (US)|
|Number of pages||7|
|Publication status||Published - 1973|
ASJC Scopus subject areas