Critical roles for IFN-β in lymphoid development, myelopoiesis, and tumor development: Links to tumor necrosis factor α

Raj Deonarain, Amit K. Verma, Andrew C G Porter, Dirk R. Gewert, Leonidas C. Platanias, Eleanor N. Fish

Research output: Contribution to journalArticle

61 Citations (Scopus)

Abstract

We have generated mice null for IFN-β and report the diverse consequences of IFN-β for both the innate and adaptive arms of immunity. Despite no abnormalities in the proportional balance of CD4 and CD8 T cell populations in the peripheral blood, thymus, and spleen of IFN-β -/- mice, activated lymph node and splenic T lymphocytes exhibit enhanced T cell proliferation and decreased tumor necrosis factor α production, relative to IFN-β+/+ mice. Notably, constitutive and induced expression of tumor necrosis factor α is reduced in the spleen and bone marrow (BM) macrophages, respectively, of IFN-β -/- mice. We also observe an altered splenic architecture in IFN-β-/- mice and a reduction in resident macrophages. We identify a potential defect in B cell maturation in IFN-β-/- mice, associated with a decrease in B220+ve/high/cD43-ve BM-derived cells and a reduction in BP-1, IgM, and CD23 expression. Circulating IgM-, Mac-1-, and Gr-1-positive cells are also substantially decreased in IFN-β-/- mice. The decrease in the numbers of circulating macrophages and granulocytes likely reflects defective maturation of primitive BM hematopoiesis in mice, shown by the reduction of colony-forming units, granulocyte-macrophage. We proceeded to evaluate the in vivo growth of malignant cells in the IFN-β-/- background and give evidence that Lewis lung carcinoma-specific tumor growth is more aggressive in IFN-β-/- mice. Taken altogether, our data suggest that, in addition to the direct growth-inhibitory effects on tumor cells, IFN-β is required during different stages of maturation in the development of the immune system.

Original languageEnglish (US)
Pages (from-to)13453-13458
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume100
Issue number23
DOIs
StatePublished - Nov 11 2003
Externally publishedYes

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Myelopoiesis
Tumor Necrosis Factor-alpha
Neoplasms
Macrophages
T-Lymphocytes
Immunoglobulin M
Spleen
Growth
Bone Marrow
Lewis Lung Carcinoma
Granulocyte-Macrophage Progenitor Cells
Hematopoiesis
Adaptive Immunity
Granulocytes
Bone Marrow Cells
Thymus Gland
Immune System
B-Lymphocytes
Lymph Nodes
Cell Proliferation

ASJC Scopus subject areas

  • Genetics
  • General

Cite this

Critical roles for IFN-β in lymphoid development, myelopoiesis, and tumor development : Links to tumor necrosis factor α. / Deonarain, Raj; Verma, Amit K.; Porter, Andrew C G; Gewert, Dirk R.; Platanias, Leonidas C.; Fish, Eleanor N.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 100, No. 23, 11.11.2003, p. 13453-13458.

Research output: Contribution to journalArticle

Deonarain, Raj ; Verma, Amit K. ; Porter, Andrew C G ; Gewert, Dirk R. ; Platanias, Leonidas C. ; Fish, Eleanor N. / Critical roles for IFN-β in lymphoid development, myelopoiesis, and tumor development : Links to tumor necrosis factor α. In: Proceedings of the National Academy of Sciences of the United States of America. 2003 ; Vol. 100, No. 23. pp. 13453-13458.
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