Correlation of the molecular electrostatic potential surface of an enzymatic transition state with novel transition-state inhibitors

Benjamin A. Horenstein, Vern L. Schramm

Research output: Contribution to journalArticle

69 Citations (Scopus)

Abstract

The transition state stabilized by nucleoside hydrolase from Crithidia fasciculata is characterized by nearly complete glycosidic bond cleavage and oxycarbonium character in the ribosyl group [Horenstein, B. A., Parkin, D. W., Estupinan, B., & Schramm, V. L. (1991) Biochemistry 30, 10788-10795]. The electrostatic potential surface of the transition state provides detailed information which should be useful in the design of transition-state analogues [Horenstein, B. A., & Schramm, V. L. (1993) Biochemistry 32, 7089-7097]. The electrostatic potential surface of inosine at the transition state contains a distributed positive charge resulting from the oxycarbonium ion character of the ribosyl ring. The ribosyl ring pucker is 3′-exo as a result of the near sp2 hybridization at C1′ of the ribose ring. A series of transition-state analogues have been synthesized which incorporate single or combined features of the transition state. Each feature of the transition state was analyzed for its contribution to binding energy. Kinetic inhibition constants correlate with the similarity of the inhibitor to the experimentally determined transition-state structure. Dissociation constants for the substrate and products of the reaction of inosine, hypoxanthine, and ribose are 380, 6200, and 700 μM, respectively. A transition-state analogue was synthesized which contains the required hydroxyl groups of the ribose ring, the positive charge feature of the oxycarbonium ion, and a hydrophobic mimic of the purine ring. The inhibitor 1(S)-phenyl-1,4-dideoxy-1,4-iminoribitol acts as a competitive inhibitor with respect to inosine with a dissociation constant of 0.17 μM. In addition, the inhibitor exhibits slow-onset inhibition which provides a final equilibrium dissociation constant of approximately 0.03 μM. The affinity of inhibitor binding correlates to the match between the electrostatic potential surfaces of the inhibitors and the transition states but less well to the geometric similarity. The results establish that an enzymatic transition state characterized by a family of kinetic isotope effects, bond vibrational analysis, and molecular electrostatic potentials facilitates the design of transition-state inhibitors.

Original languageEnglish (US)
Pages (from-to)9917-9925
Number of pages9
JournalBiochemistry
Volume32
Issue number38
StatePublished - 1993

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Static Electricity
Inosine
Ribose
Electrostatics
Biochemistry
N-Glycosyl Hydrolases
Crithidia fasciculata
Ions
Hypoxanthine
Kinetics
Corrosion inhibitors
Binding energy
Isotopes
Hydroxyl Radical
Substrates

ASJC Scopus subject areas

  • Biochemistry

Cite this

Correlation of the molecular electrostatic potential surface of an enzymatic transition state with novel transition-state inhibitors. / Horenstein, Benjamin A.; Schramm, Vern L.

In: Biochemistry, Vol. 32, No. 38, 1993, p. 9917-9925.

Research output: Contribution to journalArticle

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