TY - JOUR
T1 - Correlation between the donor age and the proliferative lifespan of human peritoneal mesothelial cells in vitro
T2 - Is TGF-β1 a link?
AU - Ksiazek, Krzysztof
AU - Winckiewicz, Marek
AU - Staniszewski, Ryszard
AU - Breborowicz, Andrzej
AU - Witowski, Janusz
PY - 2007/8
Y1 - 2007/8
N2 - In the present study we have examined the relationship between calendar age of the donor and the proliferative lifespan and TGF-β1 production by human peritoneal mesothelial cells (HPMC) in culture. The experiments were performed on primary omentum-derived HPMC isolated from patients undergoing elective abdominal surgery. There was an inverse relationship between the calendar age of the tissue donor and the replicative lifespan of HPMC in vitro (n = 49, r = -0.3991, p < 0.005). There was also a positive correlation between the donor's age and the magnitude of TGF-β1 production by first-passage HPMC (n = 28, r = 0.5400, p < 0.004). In turn, the TGF-β1 levels correlated inversely with the proliferative lifespan of HPMC in vitro (n = 28, r = -0.4671, p < 0.02). These findings indicate that the reduced proliferative capacity of HPMC isolated from older donors may be associated with increased TGF-β1 release, which may, in turn, result from the age-related accumulation of senescent HPMC in the peritoneum.
AB - In the present study we have examined the relationship between calendar age of the donor and the proliferative lifespan and TGF-β1 production by human peritoneal mesothelial cells (HPMC) in culture. The experiments were performed on primary omentum-derived HPMC isolated from patients undergoing elective abdominal surgery. There was an inverse relationship between the calendar age of the tissue donor and the replicative lifespan of HPMC in vitro (n = 49, r = -0.3991, p < 0.005). There was also a positive correlation between the donor's age and the magnitude of TGF-β1 production by first-passage HPMC (n = 28, r = 0.5400, p < 0.004). In turn, the TGF-β1 levels correlated inversely with the proliferative lifespan of HPMC in vitro (n = 28, r = -0.4671, p < 0.02). These findings indicate that the reduced proliferative capacity of HPMC isolated from older donors may be associated with increased TGF-β1 release, which may, in turn, result from the age-related accumulation of senescent HPMC in the peritoneum.
KW - Ageing
KW - Mesothelial cells
KW - Peritoneum
KW - Replicative senescence
KW - TGF-β
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U2 - 10.1016/j.exger.2007.04.012
DO - 10.1016/j.exger.2007.04.012
M3 - Article
C2 - 17587523
AN - SCOPUS:34447340230
SN - 0531-5565
VL - 42
SP - 840
EP - 843
JO - Experimental Gerontology
JF - Experimental Gerontology
IS - 8
ER -