TY - JOUR
T1 - Coronary perfusate composition influences diastolic properties, myocardial water content, and histologic characteristics of the rat left ventricle
AU - Starr, Joanne P.
AU - Jia, Chao Xiang
AU - Amirhamzeh, Mehrdad M.R.
AU - Rabkin, David G.
AU - Hart, Joseph P.
AU - Hsu, Daphne T.
AU - Fisher, Peter E.
AU - Szabolcs, Matthias
AU - Spotnitz, Henry M.
N1 - Funding Information:
This study was supported by American Heart Association Grant-In-Aid 92163. We are grateful to Robert Sciacca, Eng SciD, for statistical analysis, Michael Sardo for technical assistance, and Natalya Chalik for editorial assistance.
PY - 1999/9
Y1 - 1999/9
N2 - Background. Recent studies found that edema, histology, and left ventricular diastolic compliance exhibit quantitative relationships in rats. Edema due to low osmolarity coronary perfusates increases myocardial water content and histologic edema score and decreases left ventricular filling. The present study examined effects of perfusate osmolarity and chemical composition on rat hearts. Methods. Arrested American Cancer Institute (ACI) rat hearts (4°C) were perfused with different cardioplegia solutions, including Plegisol (289 mOsm/L), dilute Plegisol (172 mOsm/L), Stanford solution (409 mOsm/L), and University of Wisconsin solution (315 mOsm/L). Controls had blood perfusion (310 mOsm/L). Postmortem left ventricular pressure- volume curves and myocardial water content were measured. After glutaraldehyde or formalin fixation, dehydration, and paraffin embedding, edema was graded subjectively. Results. Myocardial water content reflected perfusate osmolarity, being lowest in Stanford and University of Wisconsin solutions (p < 0.05 versus other groups) and highest in dilute Plegisol (P < 0.05). Left ventricular filling volumes were smallest in dilute Plegisol and Plegisol (p < 0.05). Osmolarity was not a major determinant of myocardial edema grade, which was highest with University of Wisconsin solution and dilute Plegisol (p < 0.05 versus other groups). Conclusions. Perfusate osmolarity determined myocardial water content and left ventricular filling volume. However, perfusate chemical composition influenced the histologic appearance of edema. Pathologic grading of edema can be influenced by factors other than osmolarity alone.
AB - Background. Recent studies found that edema, histology, and left ventricular diastolic compliance exhibit quantitative relationships in rats. Edema due to low osmolarity coronary perfusates increases myocardial water content and histologic edema score and decreases left ventricular filling. The present study examined effects of perfusate osmolarity and chemical composition on rat hearts. Methods. Arrested American Cancer Institute (ACI) rat hearts (4°C) were perfused with different cardioplegia solutions, including Plegisol (289 mOsm/L), dilute Plegisol (172 mOsm/L), Stanford solution (409 mOsm/L), and University of Wisconsin solution (315 mOsm/L). Controls had blood perfusion (310 mOsm/L). Postmortem left ventricular pressure- volume curves and myocardial water content were measured. After glutaraldehyde or formalin fixation, dehydration, and paraffin embedding, edema was graded subjectively. Results. Myocardial water content reflected perfusate osmolarity, being lowest in Stanford and University of Wisconsin solutions (p < 0.05 versus other groups) and highest in dilute Plegisol (P < 0.05). Left ventricular filling volumes were smallest in dilute Plegisol and Plegisol (p < 0.05). Osmolarity was not a major determinant of myocardial edema grade, which was highest with University of Wisconsin solution and dilute Plegisol (p < 0.05 versus other groups). Conclusions. Perfusate osmolarity determined myocardial water content and left ventricular filling volume. However, perfusate chemical composition influenced the histologic appearance of edema. Pathologic grading of edema can be influenced by factors other than osmolarity alone.
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U2 - 10.1016/S0003-4975(99)00688-8
DO - 10.1016/S0003-4975(99)00688-8
M3 - Article
C2 - 10509985
AN - SCOPUS:0032701303
SN - 0003-4975
VL - 68
SP - 925
EP - 930
JO - Annals of Thoracic Surgery
JF - Annals of Thoracic Surgery
IS - 3
ER -