Core histones of the amitochondriate protist, Giardia lamblia

Gang Wu, Andrew G. McArthur, Andras Fiser, Andrej Šali, Mitchell L. Sogin, Miklós Müller

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

Genes coding for the core histones H2a, H2b, H3, and H4 of Giardia lamblia were sequenced. A conserved organism- and gene-specific element, GRGCGCAGATTVGG, was found upstream of the coding region in all core histone genes. The derived amino acid sequences of all four histones were similar to their homologs in other eukaryotes, although they were among the most divergent members of this protein family. Comparative protein structure modeling combined with energy evaluation of the resulting models indicated that the G. lamblia core histones individually and together can assume the same three-dimensional structures that were established by X-ray crystallography for Xenopus laevis histones and the nucleosome core particle. Since G. lamblia represents one of the earliest-diverging eukaryotes in many different molecular trees, the structure of its histones is potentially of relevance to understanding histone evolution. The G. lamblia proteins do not represent an intermediate stage between archaeal and eukaryotic histones.

Original languageEnglish (US)
Pages (from-to)1156-1163
Number of pages8
JournalMolecular Biology and Evolution
Volume17
Issue number8
StatePublished - 2000
Externally publishedYes

Fingerprint

Giardia lamblia
protist
histones
Histones
eukaryote
protein
gene
crystallography
Genes
amino acid
Eukaryota
eukaryotic cells
Proteins
modeling
nucleosomes
genes
energy
protists
Nucleosomes
X ray crystallography

Keywords

  • Evolution
  • Giardia lamblia
  • Histone
  • Promoter
  • Three-dimensional structure

ASJC Scopus subject areas

  • Genetics
  • Biochemistry
  • Genetics(clinical)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Ecology, Evolution, Behavior and Systematics
  • Agricultural and Biological Sciences (miscellaneous)
  • Molecular Biology

Cite this

Wu, G., McArthur, A. G., Fiser, A., Šali, A., Sogin, M. L., & Müller, M. (2000). Core histones of the amitochondriate protist, Giardia lamblia. Molecular Biology and Evolution, 17(8), 1156-1163.

Core histones of the amitochondriate protist, Giardia lamblia. / Wu, Gang; McArthur, Andrew G.; Fiser, Andras; Šali, Andrej; Sogin, Mitchell L.; Müller, Miklós.

In: Molecular Biology and Evolution, Vol. 17, No. 8, 2000, p. 1156-1163.

Research output: Contribution to journalArticle

Wu, G, McArthur, AG, Fiser, A, Šali, A, Sogin, ML & Müller, M 2000, 'Core histones of the amitochondriate protist, Giardia lamblia', Molecular Biology and Evolution, vol. 17, no. 8, pp. 1156-1163.
Wu G, McArthur AG, Fiser A, Šali A, Sogin ML, Müller M. Core histones of the amitochondriate protist, Giardia lamblia. Molecular Biology and Evolution. 2000;17(8):1156-1163.
Wu, Gang ; McArthur, Andrew G. ; Fiser, Andras ; Šali, Andrej ; Sogin, Mitchell L. ; Müller, Miklós. / Core histones of the amitochondriate protist, Giardia lamblia. In: Molecular Biology and Evolution. 2000 ; Vol. 17, No. 8. pp. 1156-1163.
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