Copper overload in Paracoccidioides lutzii results in the accumulation of ergosterol and melanin

Igor Godinho Portis, Patrícia de Sousa Lima, Rodrigo Almeida Paes, Lucas Nojosa Oliveira, Christie Ataídes Pereira, Juliana Alves Parente-Rocha, Maristela Pereira, Joshua Daniel Nosanchuk, Célia Maria de Almeida Soares

Research output: Contribution to journalArticlepeer-review

6 Scopus citations


Paracoccidioidomycosis is a highly prevalent systemic mycosis in Latin America, caused by fungi of the genus Paracoccidioides. Copper is essential for eukaryotes and bacteria. This micronutrient is used in many vital biochemical processes, although metal excess levels can be toxic for organisms. Pathways underlying copper overload are poorly understood in members of the Paracoccidioides complex. The responses of Paracoccidioides lutzii yeast cells to copper overload were here evaluated. The results showed that under copper overload, cells presented a dark brown pigment, identified as melanin. Proteomic analyses identified mainly the accumulation of proteins related to amino acids metabolism, ergosterol synthesis and melanin production, suggesting that P. lutzii responds to copper overload by changing aspects of its metabolism and also plasma membrane and cell wall remodeling. Proteomic data were confirmed by biochemical analysis.

Original languageEnglish (US)
Article number126524
JournalMicrobiological Research
StatePublished - Oct 2020


  • Cellular defense
  • Copper overload
  • Fungi
  • Proteome

ASJC Scopus subject areas

  • Microbiology


Dive into the research topics of 'Copper overload in Paracoccidioides lutzii results in the accumulation of ergosterol and melanin'. Together they form a unique fingerprint.

Cite this