TY - JOUR
T1 - Copper overload in Paracoccidioides lutzii results in the accumulation of ergosterol and melanin
AU - Portis, Igor Godinho
AU - de Sousa Lima, Patrícia
AU - Paes, Rodrigo Almeida
AU - Oliveira, Lucas Nojosa
AU - Pereira, Christie Ataídes
AU - Parente-Rocha, Juliana Alves
AU - Pereira, Maristela
AU - Nosanchuk, Joshua Daniel
AU - de Almeida Soares, Célia Maria
N1 - Funding Information:
This work was supported by grants from Instituto Nacional de Ci?ncia e Tecnologia de Estrat?gias de Intera??o Pat?geno-Hopedeiro - Funda??o de Amparo ? Pesquisa do Estado de Goi?s (FAPEG). CMAS, MP and JAPR are fellow researchers from Conselho Nacional de Desenvolvimento Cient?fico e Tecnol?gico - CNPq. JDN is partially supported by NIHAI52733. RAP is supported by CNPq grants (449184/2014-5 and 305487/2015-9). IGP, RAP, LNO and CAP and were supported by fellowships from Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior ? CAPES. The authors wish to thank Laurine Lacerda Pigosso for the technical assistance in the initial development of the Project.
Funding Information:
This work was supported by grants from Instituto Nacional de Ciência e Tecnologia de Estratégias de Interação Patógeno-Hopedeiro - Fundação de Amparo à Pesquisa do Estado de Goiás (FAPEG) . CMAS, MP and JAPR are fellow researchers from Conselho Nacional de Desenvolvimento Científico e Tecnológico - CNPq. JDN is partially supported by NIH AI52733 . RAP is supported by CNPq grants ( 449184/2014-5 and 305487/2015-9 ). IGP, RAP, LNO and CAP and were supported by fellowships from Coordenação de Aperfeiçoamento de Pessoal de Nível Superior – CAPES . The authors wish to thank Laurine Lacerda Pigosso for the technical assistance in the initial development of the Project.
Publisher Copyright:
© 2020 Elsevier GmbH
PY - 2020/10
Y1 - 2020/10
N2 - Paracoccidioidomycosis is a highly prevalent systemic mycosis in Latin America, caused by fungi of the genus Paracoccidioides. Copper is essential for eukaryotes and bacteria. This micronutrient is used in many vital biochemical processes, although metal excess levels can be toxic for organisms. Pathways underlying copper overload are poorly understood in members of the Paracoccidioides complex. The responses of Paracoccidioides lutzii yeast cells to copper overload were here evaluated. The results showed that under copper overload, cells presented a dark brown pigment, identified as melanin. Proteomic analyses identified mainly the accumulation of proteins related to amino acids metabolism, ergosterol synthesis and melanin production, suggesting that P. lutzii responds to copper overload by changing aspects of its metabolism and also plasma membrane and cell wall remodeling. Proteomic data were confirmed by biochemical analysis.
AB - Paracoccidioidomycosis is a highly prevalent systemic mycosis in Latin America, caused by fungi of the genus Paracoccidioides. Copper is essential for eukaryotes and bacteria. This micronutrient is used in many vital biochemical processes, although metal excess levels can be toxic for organisms. Pathways underlying copper overload are poorly understood in members of the Paracoccidioides complex. The responses of Paracoccidioides lutzii yeast cells to copper overload were here evaluated. The results showed that under copper overload, cells presented a dark brown pigment, identified as melanin. Proteomic analyses identified mainly the accumulation of proteins related to amino acids metabolism, ergosterol synthesis and melanin production, suggesting that P. lutzii responds to copper overload by changing aspects of its metabolism and also plasma membrane and cell wall remodeling. Proteomic data were confirmed by biochemical analysis.
KW - Cellular defense
KW - Copper overload
KW - Fungi
KW - Proteome
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U2 - 10.1016/j.micres.2020.126524
DO - 10.1016/j.micres.2020.126524
M3 - Article
C2 - 32570056
AN - SCOPUS:85086592179
SN - 0944-5013
VL - 239
JO - Microbiological Research
JF - Microbiological Research
M1 - 126524
ER -