Copper induces cellular senescence in human glioblastoma multiforme cells through downregulation of Bmi-1.

Yuan Li, Jifan Hu, Fangxia Guan, Laijun Song, Ruitai Fan, Huaijie Zhu, Xiang Hu, Eileen Shen, B. Yang

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Abstract

Most human tumor cells, including glioblastoma multiforme (GBM) cells, have aberrant control of cell aging and apoptosis. Subcytotoxic concentrations of oxidative or stress-causing agents, such as hydrogen peroxide, may induce human cell senescence. Thus, induction of tumor cells into premature senescence may provide a useful in vitro model for developing novel therapeutic strategy to combat tumors. In the present study, we assessed the molecular mechanism(s) underlying senescence in GBM cells induced by copper sulfate. Following pretreatment with subcytotoxic concentrations of copper sulfate, U87-MG tumor cells showed typical aging characteristics, including reduced cell proliferation, cell enlargement, increased level of senescence-associated β-galactosidase (SA β-gal) activity, and overexpression of several senescence-associated genes, p16, p21, transforming growth factor β-1 (TGF-β1), insulin growth factor binding protein 3 (IGFBP3) and apolipoprotein J (ApoJ). We further demonstrated that the Bmi-1 pathway was downregulated in GBM cells in parallel with the induced senescence. The present study for the first time demonstrates the ability of copper to induce GBM cell senescence by downregulating Bmi-1.

Original languageEnglish (US)
Pages (from-to)1805-1810
Number of pages6
JournalOncology Reports
Volume29
Issue number5
StatePublished - May 2013
Externally publishedYes

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Cell Aging
Glioblastoma
Copper
Down-Regulation
Copper Sulfate
Neoplasms
Cell Enlargement
Galactosidases
p16 Genes
Transforming Growth Factors
Hydrogen Peroxide
Intercellular Signaling Peptides and Proteins
Carrier Proteins
Oxidative Stress
Cell Proliferation
Insulin
Apoptosis

ASJC Scopus subject areas

  • Cancer Research
  • Oncology
  • Medicine(all)

Cite this

Copper induces cellular senescence in human glioblastoma multiforme cells through downregulation of Bmi-1. / Li, Yuan; Hu, Jifan; Guan, Fangxia; Song, Laijun; Fan, Ruitai; Zhu, Huaijie; Hu, Xiang; Shen, Eileen; Yang, B.

In: Oncology Reports, Vol. 29, No. 5, 05.2013, p. 1805-1810.

Research output: Contribution to journalArticle

Li, Y, Hu, J, Guan, F, Song, L, Fan, R, Zhu, H, Hu, X, Shen, E & Yang, B 2013, 'Copper induces cellular senescence in human glioblastoma multiforme cells through downregulation of Bmi-1.', Oncology Reports, vol. 29, no. 5, pp. 1805-1810.
Li, Yuan ; Hu, Jifan ; Guan, Fangxia ; Song, Laijun ; Fan, Ruitai ; Zhu, Huaijie ; Hu, Xiang ; Shen, Eileen ; Yang, B. / Copper induces cellular senescence in human glioblastoma multiforme cells through downregulation of Bmi-1. In: Oncology Reports. 2013 ; Vol. 29, No. 5. pp. 1805-1810.
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AU - Fan, Ruitai

AU - Zhu, Huaijie

AU - Hu, Xiang

AU - Shen, Eileen

AU - Yang, B.

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