Coordination of heparan sulfate proteoglycans with wnt signaling to control cellular migrations and positioning in caenorhabditis elegans

Kristian Saied-Santiago, Robert A. Townley, John D. Attonito, Dayse S. Da Cunha, Carlos A. Díaz-Balzac, Eillen Tecle, Hannes E. Buelow

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Heparan sulfates (HS) are linear polysaccharides with complex modification patterns, which are covalently bound via conserved attachment sites to core proteins to form heparan sulfate proteoglycans (HSPGs). HSPGs regulate many aspects of the development and function of the nervous system, including cell migration, morphology, and network connectivity. HSPGs function as cofactors for multiple signaling pathways, including the Wnt-signaling molecules and their Frizzled receptors. To investigate the functional interactions among the HSPG and Wnt networks, we conducted genetic analyses of each, and also between these networks using five cellular migrations in the nematode Caenorhabditis elegans. We find that HSPG core proteins act genetically in a combinatorial fashion dependent on the cellular contexts. Double mutant analyses reveal distinct redundancies among HSPGs for different migration events, and different cellular migrations require distinct heparan sulfate modification patterns. Our studies reveal that the transmembrane HSPG SDN-1/Syndecan functions within the migrating cell to promote cellular migrations, while the GPI-linked LON-2/ Glypican functions cell nonautonomously to establish the final cellular position. Genetic analyses with the Wnt-signaling system show that (1) a given HSPG can act with different Wnts and Frizzled receptors, and that (2) a given Wnt/Frizzled pair acts with different HSPGs in a context-dependent manner. Lastly, we find that distinct HSPG and Wnt/Frizzled combinations serve separate functions to promote cellular migration and establish position of specific neurons. Our studies suggest that HSPGs use structurally diverse glycans in coordination with Wnt-signaling pathways to control multiple cellular behaviors, including cellular and axonal migrations and, cellular positioning.

Original languageEnglish (US)
Pages (from-to)1951-1967
Number of pages17
JournalGenetics
Volume206
Issue number4
DOIs
StatePublished - 2017

Fingerprint

Heparan Sulfate Proteoglycans
Caenorhabditis elegans
Wnt Signaling Pathway
Frizzled Receptors
Heparitin Sulfate
Polysaccharides
Syndecan-2
Glypicans
Syndecan-1
Nervous System
Cell Movement
Neurons

Keywords

  • Axon guidance
  • Cell migration
  • Development
  • Heparan sulfate
  • Wnt signaling

ASJC Scopus subject areas

  • Genetics

Cite this

Coordination of heparan sulfate proteoglycans with wnt signaling to control cellular migrations and positioning in caenorhabditis elegans. / Saied-Santiago, Kristian; Townley, Robert A.; Attonito, John D.; Da Cunha, Dayse S.; Díaz-Balzac, Carlos A.; Tecle, Eillen; Buelow, Hannes E.

In: Genetics, Vol. 206, No. 4, 2017, p. 1951-1967.

Research output: Contribution to journalArticle

Saied-Santiago, Kristian ; Townley, Robert A. ; Attonito, John D. ; Da Cunha, Dayse S. ; Díaz-Balzac, Carlos A. ; Tecle, Eillen ; Buelow, Hannes E. / Coordination of heparan sulfate proteoglycans with wnt signaling to control cellular migrations and positioning in caenorhabditis elegans. In: Genetics. 2017 ; Vol. 206, No. 4. pp. 1951-1967.
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AU - Townley, Robert A.

AU - Attonito, John D.

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AU - Díaz-Balzac, Carlos A.

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AB - Heparan sulfates (HS) are linear polysaccharides with complex modification patterns, which are covalently bound via conserved attachment sites to core proteins to form heparan sulfate proteoglycans (HSPGs). HSPGs regulate many aspects of the development and function of the nervous system, including cell migration, morphology, and network connectivity. HSPGs function as cofactors for multiple signaling pathways, including the Wnt-signaling molecules and their Frizzled receptors. To investigate the functional interactions among the HSPG and Wnt networks, we conducted genetic analyses of each, and also between these networks using five cellular migrations in the nematode Caenorhabditis elegans. We find that HSPG core proteins act genetically in a combinatorial fashion dependent on the cellular contexts. Double mutant analyses reveal distinct redundancies among HSPGs for different migration events, and different cellular migrations require distinct heparan sulfate modification patterns. Our studies reveal that the transmembrane HSPG SDN-1/Syndecan functions within the migrating cell to promote cellular migrations, while the GPI-linked LON-2/ Glypican functions cell nonautonomously to establish the final cellular position. Genetic analyses with the Wnt-signaling system show that (1) a given HSPG can act with different Wnts and Frizzled receptors, and that (2) a given Wnt/Frizzled pair acts with different HSPGs in a context-dependent manner. Lastly, we find that distinct HSPG and Wnt/Frizzled combinations serve separate functions to promote cellular migration and establish position of specific neurons. Our studies suggest that HSPGs use structurally diverse glycans in coordination with Wnt-signaling pathways to control multiple cellular behaviors, including cellular and axonal migrations and, cellular positioning.

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KW - Cell migration

KW - Development

KW - Heparan sulfate

KW - Wnt signaling

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