TY - JOUR
T1 - Coordinate modulation of transfected HSV thymidine kinase and human globin genes
AU - Roginski, Raymond S.
AU - Skoultchi, Arthur I.
AU - Henthorn, Paula
AU - Smithies, Oliver
AU - Hsiung, Nancy
AU - Kucherlapati, Raju
N1 - Funding Information:
We appreciate the gifts of different DNAs from Drs . B. Forget, L . Kedes, S. McKnight, T. Maniatis, W. Marzluff and R. Alterman. Brian Morse provided technical assistance with some blots . Linda Cardenas typed the manuscript . We thank R. L. Davies for many discussions. This work is supported by grants PHS CA 34171 and MODBDF 1-806 (R. K.) PHS CA 16368 (A. S.), and PHS AM 20120, GM 20069 (0 . S .) . A . S, is supported by an Irma T . Hirschl career scientist award. R. S. R., a medical scientist trainee, is supported by a National Institutes of Health grant to the Albert Einstein College of Medicine .
PY - 1983/11
Y1 - 1983/11
N2 - We have shown that high-frequency phenotypic switching of a transfected gene is associated with alterations in chromatin structure. To examine this phenomenon further, a plasmid containing HSV thymidine kinase and human α- and γ-globin genes was transfected into mouse L cells. All three genes were expressed through utilization of their individual promoters. One of these cell lines was capable of switching to its TK- phenotype at high frequencies (8%-10%). The revertants (TK-) had no TK or globin transcripts, while the rerevertants (TK+) expressed all three genes at their original levels. We conclude that genes introduced into cells by ligated cotransfection can be regulated coordinately and that the unit of this regulated expression can be at least 20 kb long.
AB - We have shown that high-frequency phenotypic switching of a transfected gene is associated with alterations in chromatin structure. To examine this phenomenon further, a plasmid containing HSV thymidine kinase and human α- and γ-globin genes was transfected into mouse L cells. All three genes were expressed through utilization of their individual promoters. One of these cell lines was capable of switching to its TK- phenotype at high frequencies (8%-10%). The revertants (TK-) had no TK or globin transcripts, while the rerevertants (TK+) expressed all three genes at their original levels. We conclude that genes introduced into cells by ligated cotransfection can be regulated coordinately and that the unit of this regulated expression can be at least 20 kb long.
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U2 - 10.1016/0092-8674(83)90217-9
DO - 10.1016/0092-8674(83)90217-9
M3 - Article
C2 - 6313221
AN - SCOPUS:0021033755
SN - 0092-8674
VL - 35
SP - 149
EP - 155
JO - Cell
JF - Cell
IS - 1
ER -