Cooperative regulation of AJM-1 controls junctional integrity in Caenorhabditis elegans epithelia

Mathias Köppen, Jeffrey S. Simske, Paul A. Sims, Bonnie L. Firestein, David H. Hall, Anthony D. Radice, Christopher Rongo, Jeffrey D. Hardin

Research output: Contribution to journalArticle

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Abstract

The function of epithelial cell sheets depends on the integrity of specialized cell-cell junctions that connect neighbouring cells. We have characterized the novel coiled-coil protein AJM-1, which localizes to an apical junctional domain of Caenorhabditis elegans epithelia basal to the HMR-HMP (cadherin-catenin) complex. In the absence of AJM-1, the integrity of this domain is compromised. Proper AJM-1 localization requires LET-413 and DLG-1, homologues of the Drosophila tumour suppressors Scribble and Discs large, respectively. DLG-1 physically interacts with AJM-1 and is required for its normal apical distribution, and LET-413 mediates the rapid accumulation of both DLG-1 and AJM-1 in the apical domain. In the absence of both dlg-1 and let-413 function AJM-1 is almost completely lost from apical junctions in embryos, whereas HMP-1 (α-catenin) localization is only mildly affected. We conclude that LET-413 and DLG-1 cooperatively control AJM-1 localization and that AJM-1 controls the integrity of a distinct apical junctional domain in C. elegans.

Original languageEnglish (US)
Pages (from-to)983-991
Number of pages9
JournalNature Cell Biology
Volume3
Issue number11
DOIs
StatePublished - 2001

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Linear Energy Transfer
Caenorhabditis elegans
Catenins
Epithelium
Intercellular Junctions
Normal Distribution
Cadherins
Drosophila
Embryonic Structures
Epithelial Cells
Neoplasms
Proteins

ASJC Scopus subject areas

  • Cell Biology

Cite this

Köppen, M., Simske, J. S., Sims, P. A., Firestein, B. L., Hall, D. H., Radice, A. D., ... Hardin, J. D. (2001). Cooperative regulation of AJM-1 controls junctional integrity in Caenorhabditis elegans epithelia. Nature Cell Biology, 3(11), 983-991. https://doi.org/10.1038/ncb1101-983

Cooperative regulation of AJM-1 controls junctional integrity in Caenorhabditis elegans epithelia. / Köppen, Mathias; Simske, Jeffrey S.; Sims, Paul A.; Firestein, Bonnie L.; Hall, David H.; Radice, Anthony D.; Rongo, Christopher; Hardin, Jeffrey D.

In: Nature Cell Biology, Vol. 3, No. 11, 2001, p. 983-991.

Research output: Contribution to journalArticle

Köppen, M, Simske, JS, Sims, PA, Firestein, BL, Hall, DH, Radice, AD, Rongo, C & Hardin, JD 2001, 'Cooperative regulation of AJM-1 controls junctional integrity in Caenorhabditis elegans epithelia', Nature Cell Biology, vol. 3, no. 11, pp. 983-991. https://doi.org/10.1038/ncb1101-983
Köppen, Mathias ; Simske, Jeffrey S. ; Sims, Paul A. ; Firestein, Bonnie L. ; Hall, David H. ; Radice, Anthony D. ; Rongo, Christopher ; Hardin, Jeffrey D. / Cooperative regulation of AJM-1 controls junctional integrity in Caenorhabditis elegans epithelia. In: Nature Cell Biology. 2001 ; Vol. 3, No. 11. pp. 983-991.
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