Cooperation of β2- and β3-adrenergic receptors in hematopoietic progenitor cell mobilization

Simón Méndez-Ferrer, Michela Battista, Paul S. Frenette

Research output: Chapter in Book/Report/Conference proceedingConference contribution

102 Citations (Scopus)

Abstract

CXCL12/SDF-1 dynamically regulates hematopoietic stem cell (HSC) attraction in the bone marrow (BM). Circadian regulation of bone formation and HSC traffic is relayed in bone and BM by β-adrenergic receptors (β-AR) expressed on HSCs, osteoblasts, and mesenchymal stem/progenitor cells. Circadian HSC release from the BM follows rhythmic secretion of norepinephrine from nerve terminals, β3-AR activation, and Cxcl12 downregulation, possibly from reduced Sp1 nuclear content. Here, we show that β-AR stimulation in stromal cells causes Sp1 degradation, partially mediated by the 26S proteasome. Inverted trends of circulating hematopoietic progenitors and BM Cxcl12 mRNA levels change acutely after light onset, shown to induce sympathetic efferent activity. In BM stromal cells, activation of β3-AR downregulates Cxcl12, whereas β2-AR stimulation induces clock gene expression. Double deficiency in β2- and β3-ARs compromises enforced mobilization. Therefore, β2- and β3-ARs have specific roles in stromal cells and cooperate during progenitor mobilization.

Original languageEnglish (US)
Title of host publicationAnnals of the New York Academy of Sciences
Pages139-144
Number of pages6
Volume1192
DOIs
StatePublished - Mar 2010
Externally publishedYes

Publication series

NameAnnals of the New York Academy of Sciences
Volume1192
ISSN (Print)00778923
ISSN (Electronic)17496632

Fingerprint

Hematopoietic Stem Cells
Adrenergic Receptors
Bone
Bone Marrow
Mesenchymal Stromal Cells
Stem cells
Stromal Cells
Down-Regulation
Osteoblasts
Osteogenesis
Chemical activation
Norepinephrine
Gene Expression
Light
Bone and Bones
Messenger RNA
Gene expression
Clocks
Degradation

Keywords

  • β-adrenergic receptors
  • Bone marrow stromal cells
  • Circadian
  • Clock
  • CXCL12/SDF-1
  • Hematopoietic progenitor mobilization

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Méndez-Ferrer, S., Battista, M., & Frenette, P. S. (2010). Cooperation of β2- and β3-adrenergic receptors in hematopoietic progenitor cell mobilization. In Annals of the New York Academy of Sciences (Vol. 1192, pp. 139-144). (Annals of the New York Academy of Sciences; Vol. 1192). https://doi.org/10.1111/j.1749-6632.2010.05390.x

Cooperation of β2- and β3-adrenergic receptors in hematopoietic progenitor cell mobilization. / Méndez-Ferrer, Simón; Battista, Michela; Frenette, Paul S.

Annals of the New York Academy of Sciences. Vol. 1192 2010. p. 139-144 (Annals of the New York Academy of Sciences; Vol. 1192).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Méndez-Ferrer, S, Battista, M & Frenette, PS 2010, Cooperation of β2- and β3-adrenergic receptors in hematopoietic progenitor cell mobilization. in Annals of the New York Academy of Sciences. vol. 1192, Annals of the New York Academy of Sciences, vol. 1192, pp. 139-144. https://doi.org/10.1111/j.1749-6632.2010.05390.x
Méndez-Ferrer S, Battista M, Frenette PS. Cooperation of β2- and β3-adrenergic receptors in hematopoietic progenitor cell mobilization. In Annals of the New York Academy of Sciences. Vol. 1192. 2010. p. 139-144. (Annals of the New York Academy of Sciences). https://doi.org/10.1111/j.1749-6632.2010.05390.x
Méndez-Ferrer, Simón ; Battista, Michela ; Frenette, Paul S. / Cooperation of β2- and β3-adrenergic receptors in hematopoietic progenitor cell mobilization. Annals of the New York Academy of Sciences. Vol. 1192 2010. pp. 139-144 (Annals of the New York Academy of Sciences).
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