Control of carotenoid biosynthesis through a heme-based cis-trans isomerase

Jesús Beltrán, Brian Kloss, Jonathan P. Hosler, Jiafeng Geng, Aimin Liu, Anuja Modi, John H. Dawson, Masanori Sono, Maria Shumskaya, Charles Ampomah-Dwamena, James D. Love, Eleanore T. Wurtzel

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

Plants synthesize carotenoids, which are essential for plant development and survival. These metabolites also serve as essential nutrients for human health. The biosynthetic pathway for all plant carotenoids occurs in chloroplasts and other plastids and requires 15-cis-ζ-carotene isomerase (Z-ISO). It was not known whether Z-ISO catalyzes isomerization alone or in combination with other enzymes. Here we show that Z-ISO is a bona fide enzyme and integral membrane protein. Z-ISO independently catalyzes the cis-trans isomerization of the 15-15′ carbon-carbon double bond in 9,15,9′-cis-ζ-carotene to produce the substrate required by the subsequent biosynthetic-pathway enzyme. We discovered that isomerization depends upon a ferrous heme b cofactor that undergoes redox-regulated ligand switching between the heme iron and alternate Z-ISO amino acid residues. Heme b-dependent isomerization of a large hydrophobic compound in a membrane was previously undescribed. As an isomerase, Z-ISO represents a new prototype for heme b proteins and potentially uses a new chemical mechanism.

Original languageEnglish (US)
Pages (from-to)598-605
Number of pages8
JournalNature Chemical Biology
Volume11
Issue number8
DOIs
StatePublished - Aug 23 2015

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cis-trans-Isomerases
Carotenoids
Heme
Isomerases
Biosynthetic Pathways
Enzymes
Carbon
Plastids
Plant Development
Chloroplasts
Oxidation-Reduction
Membrane Proteins
Iron
Ligands
Amino Acids
Food
Membranes
Survival
Health
Proteins

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology

Cite this

Beltrán, J., Kloss, B., Hosler, J. P., Geng, J., Liu, A., Modi, A., ... Wurtzel, E. T. (2015). Control of carotenoid biosynthesis through a heme-based cis-trans isomerase. Nature Chemical Biology, 11(8), 598-605. https://doi.org/10.1038/nchembio.1840

Control of carotenoid biosynthesis through a heme-based cis-trans isomerase. / Beltrán, Jesús; Kloss, Brian; Hosler, Jonathan P.; Geng, Jiafeng; Liu, Aimin; Modi, Anuja; Dawson, John H.; Sono, Masanori; Shumskaya, Maria; Ampomah-Dwamena, Charles; Love, James D.; Wurtzel, Eleanore T.

In: Nature Chemical Biology, Vol. 11, No. 8, 23.08.2015, p. 598-605.

Research output: Contribution to journalArticle

Beltrán, J, Kloss, B, Hosler, JP, Geng, J, Liu, A, Modi, A, Dawson, JH, Sono, M, Shumskaya, M, Ampomah-Dwamena, C, Love, JD & Wurtzel, ET 2015, 'Control of carotenoid biosynthesis through a heme-based cis-trans isomerase', Nature Chemical Biology, vol. 11, no. 8, pp. 598-605. https://doi.org/10.1038/nchembio.1840
Beltrán J, Kloss B, Hosler JP, Geng J, Liu A, Modi A et al. Control of carotenoid biosynthesis through a heme-based cis-trans isomerase. Nature Chemical Biology. 2015 Aug 23;11(8):598-605. https://doi.org/10.1038/nchembio.1840
Beltrán, Jesús ; Kloss, Brian ; Hosler, Jonathan P. ; Geng, Jiafeng ; Liu, Aimin ; Modi, Anuja ; Dawson, John H. ; Sono, Masanori ; Shumskaya, Maria ; Ampomah-Dwamena, Charles ; Love, James D. ; Wurtzel, Eleanore T. / Control of carotenoid biosynthesis through a heme-based cis-trans isomerase. In: Nature Chemical Biology. 2015 ; Vol. 11, No. 8. pp. 598-605.
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