Contribution of specific Pseudomonas aeruginosa virulence factors to pathogenesis of pneumonia in a neonatal mouse model of infection

H. B. Tang, E. Dimango, R. Bryan, M. Gambello, B. H. Iglewski, J. B. Goldberg, A. Prince

Research output: Contribution to journalArticle

297 Citations (Scopus)

Abstract

We sought to identify which Pseudomonas aeruginosa products are involved in initiating respiratory tract infection. Defined mutants derived from strain PAO, i.e., PAOR1 (lasR), PAO-pmm (algC) (an LPS mutant), and AK1152 (which is Fla- and lacks functional pili), were significantly less virulent than PAO1 in a BALBc/ByJ neonatal mouse model of infection as measured by their abilities to cause acute pneumonia, bacteremia, and death. All three mutants were also less adherent to epithelial cells in an in vitro binding assay. PAOR1 and AK1152 were less able to elicit epithelial production of interleukin-8 than PAO1. LasR was found to be required for the optimal expression of neuraminidase under conditions of increased osmolarity, as might he present in certain pathological conditions. PAO-exsA::Ω, which lacks exoenzyme S expression, was fully virulent, causing at least as much pathology as PAO1. The expression of several P. aeruginosa virulence factors appears to be required to establish pulmonary infection in the neonatal mouse.

Original languageEnglish (US)
Pages (from-to)37-43
Number of pages7
JournalInfection and Immunity
Volume64
Issue number1
StatePublished - 1996
Externally publishedYes

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Virulence Factors
Pseudomonas aeruginosa
Pneumonia
Neuraminidase
Bacteremia
Infection
Interleukin-8
Respiratory Tract Infections
Osmolar Concentration
Epithelial Cells
Pathology
Lung
In Vitro Techniques
exoenzyme S

ASJC Scopus subject areas

  • Immunology

Cite this

Tang, H. B., Dimango, E., Bryan, R., Gambello, M., Iglewski, B. H., Goldberg, J. B., & Prince, A. (1996). Contribution of specific Pseudomonas aeruginosa virulence factors to pathogenesis of pneumonia in a neonatal mouse model of infection. Infection and Immunity, 64(1), 37-43.

Contribution of specific Pseudomonas aeruginosa virulence factors to pathogenesis of pneumonia in a neonatal mouse model of infection. / Tang, H. B.; Dimango, E.; Bryan, R.; Gambello, M.; Iglewski, B. H.; Goldberg, J. B.; Prince, A.

In: Infection and Immunity, Vol. 64, No. 1, 1996, p. 37-43.

Research output: Contribution to journalArticle

Tang, HB, Dimango, E, Bryan, R, Gambello, M, Iglewski, BH, Goldberg, JB & Prince, A 1996, 'Contribution of specific Pseudomonas aeruginosa virulence factors to pathogenesis of pneumonia in a neonatal mouse model of infection', Infection and Immunity, vol. 64, no. 1, pp. 37-43.
Tang, H. B. ; Dimango, E. ; Bryan, R. ; Gambello, M. ; Iglewski, B. H. ; Goldberg, J. B. ; Prince, A. / Contribution of specific Pseudomonas aeruginosa virulence factors to pathogenesis of pneumonia in a neonatal mouse model of infection. In: Infection and Immunity. 1996 ; Vol. 64, No. 1. pp. 37-43.
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