Construction of a restriction map of bacteriophage T3 DNA

Probir Sarkar, Samit Adhya, Phillip Musich, Umadas Maitra

Research output: Contribution to journalArticle

3 Scopus citations

Abstract

A restriction endonuclease cleavage map of bacteriophage T3 DNA has been constructed. The enzymes used and, within parentheses, the number of their cleavage sites on T3 DNA are: HindIII (1), XbaI (1), BglII (1), KpnI (2), MboI (9), and HpaI (17). The size and the relative location of each fragment have been established, defining an accurate physical map of T3 DNA.

Original languageEnglish (US)
Pages (from-to)161-163
Number of pages3
JournalGene
Volume12
Issue number1-2
DOIs
StatePublished - Dec 1980

Keywords

  • E. coli
  • HpaI, MboI, BglII, KpnI, HindIII, and XbaI restriction endonucleases
  • T4 polynucleotide kinase
  • exonuclease III
  • gel electrophoresis

ASJC Scopus subject areas

  • Genetics

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    Sarkar, P., Adhya, S., Musich, P., & Maitra, U. (1980). Construction of a restriction map of bacteriophage T3 DNA. Gene, 12(1-2), 161-163. https://doi.org/10.1016/0378-1119(80)90027-X