TY - JOUR
T1 - Construction of a restriction map of bacteriophage T3 DNA
AU - Sarkar, Probir
AU - Adhya, Samit
AU - Musich, Phillip
AU - Maitra, Umadas
N1 - Funding Information:
The authors thank Dr. F.W. Studier of Brookhaven National Laboratory for a gift of T3 R4 deletion mutant. This work was supported by grants from the National Institutes of Health.
PY - 1980/12
Y1 - 1980/12
N2 - A restriction endonuclease cleavage map of bacteriophage T3 DNA has been constructed. The enzymes used and, within parentheses, the number of their cleavage sites on T3 DNA are: HindIII (1), XbaI (1), BglII (1), KpnI (2), MboI (9), and HpaI (17). The size and the relative location of each fragment have been established, defining an accurate physical map of T3 DNA.
AB - A restriction endonuclease cleavage map of bacteriophage T3 DNA has been constructed. The enzymes used and, within parentheses, the number of their cleavage sites on T3 DNA are: HindIII (1), XbaI (1), BglII (1), KpnI (2), MboI (9), and HpaI (17). The size and the relative location of each fragment have been established, defining an accurate physical map of T3 DNA.
KW - E. coli
KW - HpaI, MboI, BglII, KpnI, HindIII, and XbaI restriction endonucleases
KW - T4 polynucleotide kinase
KW - exonuclease III
KW - gel electrophoresis
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U2 - 10.1016/0378-1119(80)90027-X
DO - 10.1016/0378-1119(80)90027-X
M3 - Article
C2 - 6260585
AN - SCOPUS:0019301361
SN - 0378-1119
VL - 12
SP - 161
EP - 163
JO - Gene
JF - Gene
IS - 1-2
ER -