Construction of a replication-competent murine retrovirus vector expressing the human immunodeficiency virus type 1 tat transactivator protein

Patrick J. Dillon, Jack Lenz, Craig A. Rosen

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

A replication-competent Akv murine leukemia virus-based vector encoding the human immunodeficiency virus tat cDNA under control of the simian virus 40 early promoter sequences was constructed. The simian virus 40 tat sequences were placed within the U3 region of the 3′ long terminal repeat. The resulting virus, derived by transfection, replicated efficiently in mouse NIH 3T3 cells and maintained the tat cDNA insert. It has been suggested that Tat function requires the presence of a human-specific cofactor, which is absent in murine cells. However, infection of murine cells with the Akv virus encoding tat resulted in significant transactivation of a human immunodeficiency virus long terminal repeat-driven reporter gene, indicating that human cofactors are not always required for Tat function. The vector system described may be useful for introduction of foreign genes in vivo and in whole animals when virus spread is required for efficient infection and levels of gene expression.

Original languageEnglish (US)
Pages (from-to)4490-4493
Number of pages4
JournalJournal of Virology
Volume65
Issue number8
StatePublished - Aug 1991

Fingerprint

Retroviridae
tat Gene Products
Trans-Activators
Human immunodeficiency virus 1
Simian virus 40
HIV-1
terminal repeat sequences
Human immunodeficiency virus
Viruses
mice
Complementary DNA
Murine leukemia virus
HIV Long Terminal Repeat
Murine Leukemia Viruses
NIH 3T3 Cells
viruses
proteins
Terminal Repeat Sequences
cells
transcriptional activation

ASJC Scopus subject areas

  • Immunology

Cite this

Construction of a replication-competent murine retrovirus vector expressing the human immunodeficiency virus type 1 tat transactivator protein. / Dillon, Patrick J.; Lenz, Jack; Rosen, Craig A.

In: Journal of Virology, Vol. 65, No. 8, 08.1991, p. 4490-4493.

Research output: Contribution to journalArticle

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