Conserved machinery of the bacterial flagellar motor

A. Stahlberg, S. C. Schuster, M. Bauer, E. Baeuerlein, Rongbao Zhao, T. S. Reese, S. M. Khan, G. Elliott, C. Schutt, S. Block, Y. Goldman, S. Leibler

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Novel periplasmic and cytoplasmic structural modules of the bases of bacterial flagella have been observed in situ and isolated using new biochemical protocols. Flagellar rotation may depend upon interactions of these modules with the intramembrane particle rings, a ubiquitous feature of flagallar bases necessary for torque generation. The outer membrane- associated basal disk of the Wolinella succinogenes polar flagellum has architecture well suited for interaction with the ring particles. However, antibody against the main W. succinogenes basal disk protein did not cross- react with flagella-enriched fractions from Salmonella typhimurium and Bacillus firmus; nor have such structures been observed in these species thus far. Antibodies against two S. typhimurium proteins, FliG and FliM, known to be involved in motor function and part of the cytoplasmic module in this species cross-reacted with flagella-enriched fractions from both W. succinogenes and B. firmus. In addition, flagellar cytoplasmic structure could be isolated from B. firmus. The basal disk may anchor the flagellar motor to the cell wall in some polar bacteria, but this does not seem to be a unique strategy. In contrast, the data indicate that the cytoplasmic module is conserved.

Original languageEnglish (US)
JournalBiophysical Journal
Volume68
Issue number4 SUPPL.
StatePublished - 1995

Fingerprint

Wolinella
Flagella
Cytoplasmic Structures
Antibodies
Torque
Salmonella typhimurium
Cell Wall
Bacteria
Membranes
Bacillus firmus
Proteins

ASJC Scopus subject areas

  • Biophysics

Cite this

Stahlberg, A., Schuster, S. C., Bauer, M., Baeuerlein, E., Zhao, R., Reese, T. S., ... Leibler, S. (1995). Conserved machinery of the bacterial flagellar motor. Biophysical Journal, 68(4 SUPPL.).

Conserved machinery of the bacterial flagellar motor. / Stahlberg, A.; Schuster, S. C.; Bauer, M.; Baeuerlein, E.; Zhao, Rongbao; Reese, T. S.; Khan, S. M.; Elliott, G.; Schutt, C.; Block, S.; Goldman, Y.; Leibler, S.

In: Biophysical Journal, Vol. 68, No. 4 SUPPL., 1995.

Research output: Contribution to journalArticle

Stahlberg, A, Schuster, SC, Bauer, M, Baeuerlein, E, Zhao, R, Reese, TS, Khan, SM, Elliott, G, Schutt, C, Block, S, Goldman, Y & Leibler, S 1995, 'Conserved machinery of the bacterial flagellar motor', Biophysical Journal, vol. 68, no. 4 SUPPL..
Stahlberg A, Schuster SC, Bauer M, Baeuerlein E, Zhao R, Reese TS et al. Conserved machinery of the bacterial flagellar motor. Biophysical Journal. 1995;68(4 SUPPL.).
Stahlberg, A. ; Schuster, S. C. ; Bauer, M. ; Baeuerlein, E. ; Zhao, Rongbao ; Reese, T. S. ; Khan, S. M. ; Elliott, G. ; Schutt, C. ; Block, S. ; Goldman, Y. ; Leibler, S. / Conserved machinery of the bacterial flagellar motor. In: Biophysical Journal. 1995 ; Vol. 68, No. 4 SUPPL.
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