Conserved composition of mammalian box H/ACA and box C/D small nucleolar ribonucleoprotein particles and their interaction with the common factor Nopp140

Yunfeng Yang, Cynthia Isaac, Chen Wang, François Dragon, Vanda Pogačić, U. Thomas Meier

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Abstract

Small nucleolar ribonucleoprotein particles (snoRNPs) mainly catalyze the modification of rRNA. The two major classes of snoRNPs, box H/ACA and box C/D, function in the pseudouridylation and 2'-O-methylation, respectively, of specific nucleotides. The emerging view based on studies in yeast is that each class of snoRNPs is composed of a unique set of proteins. Here we present a characterization of mammalian snoRNPs. We show that the previously characterized NAP57 is specific for box H/ACA SnoRNPs, whereas the newly identified NAP65, the rat homologue of yeast Nop5/58p, is a component of the box C/D class. Using coimmunoprecipitation experiments, we show that the nucleolar and coiled-body protein Nopp140 interacts with both classes of snoRNPs. This interaction is corroborated in vivo by the exclusive depletion of snoRNP proteins from nucleoli in cells transfected with a dominant negative Nopp140 construct. Interestingly, RNA polymerase I transcription is arrested in nucleoli depleted of snoRNPs, raising the possibility of a feedback mechanism between rRNA modification and transcription. Moreover, the Nopp140-snoRNP interaction appears to be conserved in yeast, because depletion of Srp40p, the yeast Nopp140 homologue, in a conditional lethal strain induces the loss of box H/ACA small nucleolar RNAs. We propose that Nopp140 functions as a chaperone of snoRNPs in yeast and vertebrate cells.

Original languageEnglish (US)
Pages (from-to)567-577
Number of pages11
JournalMolecular Biology of the Cell
Volume11
Issue number2
StatePublished - Feb 2000

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Small Nucleolar Ribonucleoproteins
Yeasts
Coiled Bodies
Cell Nucleolus
Small Nucleolar RNA
RNA Polymerase I
Proteins
Methylation
Vertebrates
Nucleotides

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Cell Biology

Cite this

Conserved composition of mammalian box H/ACA and box C/D small nucleolar ribonucleoprotein particles and their interaction with the common factor Nopp140. / Yang, Yunfeng; Isaac, Cynthia; Wang, Chen; Dragon, François; Pogačić, Vanda; Meier, U. Thomas.

In: Molecular Biology of the Cell, Vol. 11, No. 2, 02.2000, p. 567-577.

Research output: Contribution to journalArticle

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