Conservation of anatomically restricted glycosaminoglycan structures in divergent nematode species

Matthew Attreed, Kristian Saied-Santiago, Hannes E. Buelow

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Heparan sulfates (HS) are glycosaminoglycans of the extracellular matrices and characterized by complex modification patterns owing to sulfations, epimerization, and acetylation. Distinct HS modification patterns have been shown to modulate protein-protein interactions during development in general and of the nervous system in particular. This has led to the heparan sulfate code hypothesis, which posits that specifically modified HS epitopes are distributed in a tissue and cell-specific fashion to orchestrate neural circuit formation. Whether an HS code exists in vivo, how specific or how evolutionarily conserved the anatomical distribution of an HS code may be has remained unknown. Here we conduct a systematic comparison of HS modification patterns in the nematode Caenorhabditis elegans using transgenic expression of 33 different HS-specific single chain variable fragment antibodies. We find that some HS modification patterns are widely distributed in the nervous system. In contrast, other HS modification patterns appear highly cell-specific in both non-neuronal and neuronal cells. Some patterns can be as restricted in their localization as to single neurites or synaptic connections between two neurons. This restricted anatomical localization of specific HS patterns can be evolutionarily conserved over a span of 80-100 million years in the divergent nematode species Caenorhabditis briggsae suggesting structural and, possibly functional conservation of glycosaminoglycan structures similar to proteins. These findings suggest a HS code with subcellularly localized, unique glycan identities in the nervous system.

Original languageEnglish (US)
Pages (from-to)862-870
Number of pages9
JournalGlycobiology
Volume26
Issue number8
DOIs
StatePublished - Aug 1 2016

Fingerprint

Heparitin Sulfate
Glycosaminoglycans
Conservation
Neurology
Nervous System
Caenorhabditis
Acetylation
Single-Chain Antibodies
Proteins
Caenorhabditis elegans
Neurites
Neurons
Extracellular Matrix
Polysaccharides
Epitopes

Keywords

  • Caenorhabditis elegans
  • conservation
  • glycosaminoglycan
  • heparin
  • synapse

ASJC Scopus subject areas

  • Biochemistry

Cite this

Conservation of anatomically restricted glycosaminoglycan structures in divergent nematode species. / Attreed, Matthew; Saied-Santiago, Kristian; Buelow, Hannes E.

In: Glycobiology, Vol. 26, No. 8, 01.08.2016, p. 862-870.

Research output: Contribution to journalArticle

Attreed, Matthew ; Saied-Santiago, Kristian ; Buelow, Hannes E. / Conservation of anatomically restricted glycosaminoglycan structures in divergent nematode species. In: Glycobiology. 2016 ; Vol. 26, No. 8. pp. 862-870.
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