Connexin43 and Pannexin1 channels in osteoblasts: Who is the "hemichannel"?

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34 Scopus citations

Abstract

Osteoblasts sense and respond to mechanical stimuli in a process involving influx and release of large ions and signaling molecules. Unapposed gap junction hemichannels formed of connexin43 (Cx43) have been proposed as a major route for such exchange, in particular for release of ATP and prostaglandin E 2 (PGE2) in osteocytes. However, we have found that Cx43-null osteoblasts have unaltered, mechanically induced PGE2 release and ATP-induced YoPro dye uptake. In contrast, PGE2 release in response to fluid shear stress is abolished in P2X7 receptor (P2X7R)-null osteoblasts, and ATP-induced dye uptake is attenuated following treatment of wild-type cells with a P2X7R or Pannexin1 (Panx1) channel blocker. These data indicate that Panx1 channels, in concert with P2X7R, likely form a molecular complex that performs the hemichannel function in osteoblast mechanosignaling.

Original languageEnglish (US)
Pages (from-to)401-409
Number of pages9
JournalJournal of Membrane Biology
Volume245
Issue number7
DOIs
StatePublished - Jul 1 2012

Keywords

  • ATP
  • Dye uptake
  • Gap junction
  • Osteoblast
  • P2XR

ASJC Scopus subject areas

  • Biophysics
  • Physiology
  • Cell Biology

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