Connexin35 mediates electrical transmission at mixed synapses on Mauthner cells

Alberto E. Pereda, J. O'Brien, J. I. Nagy, F. Bukauskas, K. G V Davidson, N. Kamasawa, T. Yasumura, J. E. Rash

Research output: Contribution to journalArticle

89 Citations (Scopus)

Abstract

Auditory afferents terminating as "large myelinated club endings" on goldfish Mauthner cells are identifiable "mixed" (electrical and chemical) synaptic terminals that offer the unique opportunity to correlate physiological properties with biochemical composition and specific ultrastructural features of individual synapses. By combining confocal microscopy and freeze-fracture replica immunogold labeling (FRIL), we demonstrate that gap junctions at these synapses contain connexin35 (Cx35). This connexin is the fish ortholog of the neuron-specific human and mouse connexin36 that is reported to be widely distributed in mammalian brain and to be responsible for electrical coupling between many types of neurons. Similarly, connexin35 was found at gap junctions between neurons in other brain regions, suggesting that connexin35-mediated electrical transmission is common in goldfish brain. Conductance of gap junction channels at large myelinated club endings is known to be dynamically modulated by the activity of their colocalized glutamatergic synapses. We show evidence by confocal microscopy for the presence of the NR1 subunit of the NMDA glutamate receptor subtype, proposed to be a key regulatory element, at these large endings. Furthermore, we also show evidence by FRIL double-immunogold labeling that the NR1 subunit of the NMDA glutamate receptor is present at postsynaptic densities closely associated with gap junction plaques containing Cx35 at mixed synapses across the goldfish hindbrain. Given the widespread distribution of electrical synapses and glutamate receptors, our results suggest that the plastic properties observed at these identifiable junctions may apply to other electrical synapses, including those in mammalian brain.

Original languageEnglish (US)
Pages (from-to)7489-7503
Number of pages15
JournalJournal of Neuroscience
Volume23
Issue number20
StatePublished - Aug 20 2003

Fingerprint

Gap Junctions
Synapses
Goldfish
Glutamate Receptors
Electrical Synapses
Brain
Neurons
Confocal Microscopy
Post-Synaptic Density
Rhombencephalon
Connexins
Presynaptic Terminals
Plastics
Fishes
NR1 NMDA receptor

Keywords

  • Auditory
  • Connexin36
  • Electrical coupling
  • Electrical synapse
  • Gap junction
  • NMDA
  • Synaptic plasticity

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Pereda, A. E., O'Brien, J., Nagy, J. I., Bukauskas, F., Davidson, K. G. V., Kamasawa, N., ... Rash, J. E. (2003). Connexin35 mediates electrical transmission at mixed synapses on Mauthner cells. Journal of Neuroscience, 23(20), 7489-7503.

Connexin35 mediates electrical transmission at mixed synapses on Mauthner cells. / Pereda, Alberto E.; O'Brien, J.; Nagy, J. I.; Bukauskas, F.; Davidson, K. G V; Kamasawa, N.; Yasumura, T.; Rash, J. E.

In: Journal of Neuroscience, Vol. 23, No. 20, 20.08.2003, p. 7489-7503.

Research output: Contribution to journalArticle

Pereda, AE, O'Brien, J, Nagy, JI, Bukauskas, F, Davidson, KGV, Kamasawa, N, Yasumura, T & Rash, JE 2003, 'Connexin35 mediates electrical transmission at mixed synapses on Mauthner cells', Journal of Neuroscience, vol. 23, no. 20, pp. 7489-7503.
Pereda AE, O'Brien J, Nagy JI, Bukauskas F, Davidson KGV, Kamasawa N et al. Connexin35 mediates electrical transmission at mixed synapses on Mauthner cells. Journal of Neuroscience. 2003 Aug 20;23(20):7489-7503.
Pereda, Alberto E. ; O'Brien, J. ; Nagy, J. I. ; Bukauskas, F. ; Davidson, K. G V ; Kamasawa, N. ; Yasumura, T. ; Rash, J. E. / Connexin35 mediates electrical transmission at mixed synapses on Mauthner cells. In: Journal of Neuroscience. 2003 ; Vol. 23, No. 20. pp. 7489-7503.
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