Conditionally replicating mycobacteriophages: A system for transposon delivery to Mycobacterium tuberculosis

Stoyan Bardarov, Jordan Kriakov, Christian Carriere, Shengwei Yu, Carlos Vaamonde, Ruth A. Mcadam, Barry R. Bloom, Graham F. Hatfull, William R. Jacobs

Research output: Contribution to journalArticle

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Abstract

Transposon mutagenesis provides a direct selection for mutants and is an extremely powerful technique to analyze genetic functions in a variety of prokaryotes. Transposon mutagenesis of Mycobacterium tuberculosis has been limited in part because of the inefficiency of the delivery systems. This report describes the development of conditionally replicating shuttle phasmids from the mycobacteriophages D29 and TM4 that enable efficient delivery of transposons into both fast- and slow-growing mycobacteria. These shuttle phasmids consist of an Escherichia coli cosmid vector containing either a mini-Tn10(kan) or Tn5367 inserted into a nonessential region of the phage genome. Thermosensitive mutations were created in the mycobacteriophage genome that allow replication at 30°C but not at 37°C (TM4) or 38.5°C (D29). Infection of mycobacteria at the nonpermissive temperature results in highly efficient transposon delivery to the entire population of mycobacterial cells. Transposition of mini-Tn10(kan) occurred in a site- specific fashion in M. smegmatis whereas Tn5367 transposed apparently randomly in M. phlei, Bacille Calmette-Guerin (BCG), and M. tuberculosis. Sequence analysis of the M. tuberculosis and BCG chromosomal regions adjacent to Tn5367 insertions, in combination with M. tuberculosis genomic sequence and physical map data, indicates that the transpositions have occurred randomly in diverse genes in every quadrant of the genome. Using this system, it has been readily possible to generate libraries containing thousands of independent mutants of M. phlei, BCG, and M. tuberculosis.

Original languageEnglish (US)
Pages (from-to)10961-10966
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume94
Issue number20
DOIs
StatePublished - Sep 30 1997

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Mycobacteriophages
Mycobacterium tuberculosis
Genome
Mutagenesis
Smegma
Cosmids
Mycobacterium Infections
Mycobacterium
Bacteriophages
Libraries
Sequence Analysis
Escherichia coli
Mutation
Temperature
Population
Genes

ASJC Scopus subject areas

  • General

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Conditionally replicating mycobacteriophages : A system for transposon delivery to Mycobacterium tuberculosis. / Bardarov, Stoyan; Kriakov, Jordan; Carriere, Christian; Yu, Shengwei; Vaamonde, Carlos; Mcadam, Ruth A.; Bloom, Barry R.; Hatfull, Graham F.; Jacobs, William R.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 94, No. 20, 30.09.1997, p. 10961-10966.

Research output: Contribution to journalArticle

Bardarov, Stoyan ; Kriakov, Jordan ; Carriere, Christian ; Yu, Shengwei ; Vaamonde, Carlos ; Mcadam, Ruth A. ; Bloom, Barry R. ; Hatfull, Graham F. ; Jacobs, William R. / Conditionally replicating mycobacteriophages : A system for transposon delivery to Mycobacterium tuberculosis. In: Proceedings of the National Academy of Sciences of the United States of America. 1997 ; Vol. 94, No. 20. pp. 10961-10966.
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