Conditional deletion of the colony stimulating factor-1 receptor (c-fms proto-oncogene) in mice

Jia Li, Ken Chen, Liyin Zhu, Jeffrey W. Pollard

Research output: Contribution to journalArticle

57 Citations (Scopus)

Abstract

Colony stimulating factor-1 (CSF-1) is the primary regulator of the mononuclear phagocytic lineage acting through its transmembrane tyrosine kinase receptor, CSF-1R, that is the product of the c-fms protooncogene. Null mutations in either the ligand or the receptor genes result in a severe osteopetrosis as well as a number of other phenotypes, including reproductive defects and perturbations in organ development. The CSF-1R is also expressed in oocytes, myoblast progenitors, decidual, and trophoblastic cells. To distinguish cell type specific phenotypes, we have created a conditional allele of the Csf1r by placing LoxP sites around Exon 5 of the Csf1r gene in mice. Excision of this floxed sequence results in a null allele that in the homozygous state gives a phenotype indistinguishable of the complete Csf1r null mutant mouse. This conditional allele will prove extremely valuable to study the spatial and temporal roles of CSF-1R.

Original languageEnglish (US)
Pages (from-to)328-335
Number of pages8
JournalGenesis
Volume44
Issue number7
DOIs
StatePublished - Jul 2006

Fingerprint

fms Genes
Colony-Stimulating Factor Receptors
Macrophage Colony-Stimulating Factor
Alleles
Phenotype
Osteopetrosis
Myoblasts
Receptor Protein-Tyrosine Kinases
Genes
Oocytes
Exons
Ligands
Mutation

Keywords

  • CSF-1
  • M-CSF
  • Macrophages
  • Reproduction

ASJC Scopus subject areas

  • Genetics

Cite this

Conditional deletion of the colony stimulating factor-1 receptor (c-fms proto-oncogene) in mice. / Li, Jia; Chen, Ken; Zhu, Liyin; Pollard, Jeffrey W.

In: Genesis, Vol. 44, No. 7, 07.2006, p. 328-335.

Research output: Contribution to journalArticle

Li, Jia ; Chen, Ken ; Zhu, Liyin ; Pollard, Jeffrey W. / Conditional deletion of the colony stimulating factor-1 receptor (c-fms proto-oncogene) in mice. In: Genesis. 2006 ; Vol. 44, No. 7. pp. 328-335.
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