Compromised microcirculation in acute murine Trypanosoma cruzi infection

Herbert B. Tanowitz, Dhananjaya K. Kaul, Bing Chen, Stephen A. Morris, Stephen M. Factor, Louis M. Weiss, Murray Wittner

Research output: Contribution to journalArticle

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Abstract

Microvascular compromise has been 1 of many factors implicated in the etiology of the cardiomyopathy associated with Chagas' disease. To further assess the effect of Trypanosoma cruzi infection on the microcirculation, we examined the cremaster microvascular model in CD-1 male mice infected with the Brazil strain at 20-25 days postinfection. There was a significant decrease in red cell velocity (Vrbc) in first and third-order arterioles and venules, which was reversed by verapamil treatment. Video recordings revealed a marked inflammatory response that was confirmed by transmission electron microscopy. A marked inflammatory response was not seen in verapamil-treated infected mice. Segmental vasospasm and dilatation was evident in the microvascular bed of infected mice. This was not seen in control or verapamil-treated mice. This model provides a readily accessible method to observe directly the effects of T. cruzi infection on the microcirculatory flow in vivo. In addition, it confirms and extends our previous observations regarding T. cruzi-associated microvascular spasm and underscores a role for verapamil, a calcium-channel blocker, in the amelioration of the Chagas' disease.

Original languageEnglish (US)
Pages (from-to)124-130
Number of pages7
JournalJournal of Parasitology
Volume82
Issue number1
DOIs
StatePublished - Feb 1996

Fingerprint

Chagas disease
verapamil
Trypanosoma cruzi
Microcirculation
Verapamil
Chagas Disease
etiology
mice
Infection
infection
transmission electron microscopy
remediation
inflammation
calcium
calcium channel blockers
Video Recording
Venules
cardiomyopathy
Calcium Channel Blockers
Spasm

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Parasitology
  • Microbiology

Cite this

Tanowitz, H. B., Kaul, D. K., Chen, B., Morris, S. A., Factor, S. M., Weiss, L. M., & Wittner, M. (1996). Compromised microcirculation in acute murine Trypanosoma cruzi infection. Journal of Parasitology, 82(1), 124-130. https://doi.org/10.2307/3284127

Compromised microcirculation in acute murine Trypanosoma cruzi infection. / Tanowitz, Herbert B.; Kaul, Dhananjaya K.; Chen, Bing; Morris, Stephen A.; Factor, Stephen M.; Weiss, Louis M.; Wittner, Murray.

In: Journal of Parasitology, Vol. 82, No. 1, 02.1996, p. 124-130.

Research output: Contribution to journalArticle

Tanowitz, HB, Kaul, DK, Chen, B, Morris, SA, Factor, SM, Weiss, LM & Wittner, M 1996, 'Compromised microcirculation in acute murine Trypanosoma cruzi infection', Journal of Parasitology, vol. 82, no. 1, pp. 124-130. https://doi.org/10.2307/3284127
Tanowitz, Herbert B. ; Kaul, Dhananjaya K. ; Chen, Bing ; Morris, Stephen A. ; Factor, Stephen M. ; Weiss, Louis M. ; Wittner, Murray. / Compromised microcirculation in acute murine Trypanosoma cruzi infection. In: Journal of Parasitology. 1996 ; Vol. 82, No. 1. pp. 124-130.
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