Comprehensive proteomic analysis of membrane proteins in Toxoplasma gondii

Fa Yun Che, Carlos Madrid-Aliste, Berta Burd, Hongshan Zhang, Edward Nieves, Kami Kim, Andras Fiser, Ruth Hogue Angeletti, Louis M. Weiss

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

Toxoplasma gondii (T. gondii) is an obligate intracellular protozoan parasite that is an important human and animal pathogen. Experimental information on T. gondii membrane proteins is limited, and the majority of gene predictions with predicted transmembrane motifs are of unknown function. A systematic analysis of the membrane proteome of T. gondii is important not only for understanding this parasite's invasion mechanism(s), but also for the discovery of potential drug targets and new preventative and therapeutic strategies. Here we report a comprehensive analysis of the membrane proteome of T. gondii, employing three proteomics strategies: one-dimensional gel liquid chromatography-tandem MS analysis (one-dimensional gel electrophoresis LC-MS/MS), biotin labeling in conjunction with one-dimensional gel LC-MS/MS analysis, and a novel strategy that combines three-layer "sandwich" gel electrophoresis with multidimensional protein identification technology. A total of 2241 T. gondii proteins with at least one predicted transmembrane segment were identified and grouped into 841 sequentially nonredundant protein clusters, which account for 21.8% of the predicted transmembrane protein clusters in the T. gondii genome. A large portion (42%) of the identified T. gondii membrane proteins are hypothetical proteins. Furthermore, many of the membrane proteins validated by mass spectrometry are unique to T. gondii or to the Apicomplexa, providing a set of gene predictions ripe for experimental investigation, and potentially suitable targets for the development of therapeutic strategies.

Original languageEnglish (US)
JournalMolecular and Cellular Proteomics
Volume10
Issue number1
DOIs
StatePublished - Jan 2011

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Toxoplasma
Proteomics
Membrane Proteins
Gels
Genes
Proteome
Proteins
Electrophoresis
Membranes
Liquid chromatography
Pathogens
Biotin
Parasites
Labeling
Mass spectrometry
Apicomplexa
Animals
Drug Discovery
Liquid Chromatography
Gel Chromatography

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Analytical Chemistry
  • Medicine(all)

Cite this

Comprehensive proteomic analysis of membrane proteins in Toxoplasma gondii. / Che, Fa Yun; Madrid-Aliste, Carlos; Burd, Berta; Zhang, Hongshan; Nieves, Edward; Kim, Kami; Fiser, Andras; Angeletti, Ruth Hogue; Weiss, Louis M.

In: Molecular and Cellular Proteomics, Vol. 10, No. 1, 01.2011.

Research output: Contribution to journalArticle

Che, Fa Yun ; Madrid-Aliste, Carlos ; Burd, Berta ; Zhang, Hongshan ; Nieves, Edward ; Kim, Kami ; Fiser, Andras ; Angeletti, Ruth Hogue ; Weiss, Louis M. / Comprehensive proteomic analysis of membrane proteins in Toxoplasma gondii. In: Molecular and Cellular Proteomics. 2011 ; Vol. 10, No. 1.
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