Comprehensive miRNA Profiling of Skeletal Muscle and Serum in Induced and Normal Mouse Muscle Atrophy During Aging

Hwa Jin Jung, Kwang Pyo Lee, Brandon Milholland, Yeo Jin Shin, Jae Sook Kang, Ki Sun Kwon, Yousin Suh

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Age-associated loss of muscle mass and function is a major cause of morbidity and mortality in the elderly adults. Muscular atrophy can also be induced by disuse associated with long-term bed rest or disease. Although miRNAs regulate muscle growth, regeneration, and aging, their potential role in acute muscle atrophy is poorly understood. Furthermore, alterations in circulating miRNA levels have been shown to occur during aging but their potential as noninvasive biomarkers for muscle atrophy remains largely unexplored. Here, we report comprehensive miRNA expression profiles by miRNA-seq analysis in tibialis anterior muscle and serum of a disuse-induced atrophy mouse model, mimicking the acute atrophy following long-term bed rest, as compared to those of young and old mice. Comparative analysis and validation studies have revealed that miR-455-3p was significantly decreased in muscle of both induced-atrophy model and old mice, whereas miR-434-3p was decreased in both serum and muscle of old mice, as compared to young mice. Furthermore, upregulation of miR-455-3p in fully differentiated C2C12 myoblasts induced a hypertrophic phenotype. These results suggest that deregulation of miR-455-3p may play a functional role in muscle atrophy and miR-434-3p could be a candidate serum biomarker of muscle aging.

Original languageEnglish (US)
Pages (from-to)1483-1491
Number of pages9
JournalThe journals of gerontology. Series A, Biological sciences and medical sciences
Volume72
Issue number11
DOIs
StatePublished - Oct 12 2017

Fingerprint

Muscular Atrophy
MicroRNAs
Skeletal Muscle
Muscles
Serum
Bed Rest
Atrophy
Biomarkers
Atrophic Muscular Disorders
Myoblasts
Validation Studies
Regeneration
Up-Regulation
Morbidity
Phenotype
Mortality
Growth

Keywords

  • Disuse muscle atrophy
  • MicroRNA
  • Muscle aging
  • Sarcopenia
  • Serum

ASJC Scopus subject areas

  • Aging
  • Geriatrics and Gerontology

Cite this

Comprehensive miRNA Profiling of Skeletal Muscle and Serum in Induced and Normal Mouse Muscle Atrophy During Aging. / Jung, Hwa Jin; Lee, Kwang Pyo; Milholland, Brandon; Shin, Yeo Jin; Kang, Jae Sook; Kwon, Ki Sun; Suh, Yousin.

In: The journals of gerontology. Series A, Biological sciences and medical sciences, Vol. 72, No. 11, 12.10.2017, p. 1483-1491.

Research output: Contribution to journalArticle

@article{42e0180a13024e428b85e6e0619a5383,
title = "Comprehensive miRNA Profiling of Skeletal Muscle and Serum in Induced and Normal Mouse Muscle Atrophy During Aging",
abstract = "Age-associated loss of muscle mass and function is a major cause of morbidity and mortality in the elderly adults. Muscular atrophy can also be induced by disuse associated with long-term bed rest or disease. Although miRNAs regulate muscle growth, regeneration, and aging, their potential role in acute muscle atrophy is poorly understood. Furthermore, alterations in circulating miRNA levels have been shown to occur during aging but their potential as noninvasive biomarkers for muscle atrophy remains largely unexplored. Here, we report comprehensive miRNA expression profiles by miRNA-seq analysis in tibialis anterior muscle and serum of a disuse-induced atrophy mouse model, mimicking the acute atrophy following long-term bed rest, as compared to those of young and old mice. Comparative analysis and validation studies have revealed that miR-455-3p was significantly decreased in muscle of both induced-atrophy model and old mice, whereas miR-434-3p was decreased in both serum and muscle of old mice, as compared to young mice. Furthermore, upregulation of miR-455-3p in fully differentiated C2C12 myoblasts induced a hypertrophic phenotype. These results suggest that deregulation of miR-455-3p may play a functional role in muscle atrophy and miR-434-3p could be a candidate serum biomarker of muscle aging.",
keywords = "Disuse muscle atrophy, MicroRNA, Muscle aging, Sarcopenia, Serum",
author = "Jung, {Hwa Jin} and Lee, {Kwang Pyo} and Brandon Milholland and Shin, {Yeo Jin} and Kang, {Jae Sook} and Kwon, {Ki Sun} and Yousin Suh",
year = "2017",
month = "10",
day = "12",
doi = "10.1093/gerona/glx025",
language = "English (US)",
volume = "72",
pages = "1483--1491",
journal = "Journals of Gerontology - Series A Biological Sciences and Medical Sciences",
issn = "1079-5006",
publisher = "Oxford University Press",
number = "11",

}

TY - JOUR

T1 - Comprehensive miRNA Profiling of Skeletal Muscle and Serum in Induced and Normal Mouse Muscle Atrophy During Aging

AU - Jung, Hwa Jin

AU - Lee, Kwang Pyo

AU - Milholland, Brandon

AU - Shin, Yeo Jin

AU - Kang, Jae Sook

AU - Kwon, Ki Sun

AU - Suh, Yousin

PY - 2017/10/12

Y1 - 2017/10/12

N2 - Age-associated loss of muscle mass and function is a major cause of morbidity and mortality in the elderly adults. Muscular atrophy can also be induced by disuse associated with long-term bed rest or disease. Although miRNAs regulate muscle growth, regeneration, and aging, their potential role in acute muscle atrophy is poorly understood. Furthermore, alterations in circulating miRNA levels have been shown to occur during aging but their potential as noninvasive biomarkers for muscle atrophy remains largely unexplored. Here, we report comprehensive miRNA expression profiles by miRNA-seq analysis in tibialis anterior muscle and serum of a disuse-induced atrophy mouse model, mimicking the acute atrophy following long-term bed rest, as compared to those of young and old mice. Comparative analysis and validation studies have revealed that miR-455-3p was significantly decreased in muscle of both induced-atrophy model and old mice, whereas miR-434-3p was decreased in both serum and muscle of old mice, as compared to young mice. Furthermore, upregulation of miR-455-3p in fully differentiated C2C12 myoblasts induced a hypertrophic phenotype. These results suggest that deregulation of miR-455-3p may play a functional role in muscle atrophy and miR-434-3p could be a candidate serum biomarker of muscle aging.

AB - Age-associated loss of muscle mass and function is a major cause of morbidity and mortality in the elderly adults. Muscular atrophy can also be induced by disuse associated with long-term bed rest or disease. Although miRNAs regulate muscle growth, regeneration, and aging, their potential role in acute muscle atrophy is poorly understood. Furthermore, alterations in circulating miRNA levels have been shown to occur during aging but their potential as noninvasive biomarkers for muscle atrophy remains largely unexplored. Here, we report comprehensive miRNA expression profiles by miRNA-seq analysis in tibialis anterior muscle and serum of a disuse-induced atrophy mouse model, mimicking the acute atrophy following long-term bed rest, as compared to those of young and old mice. Comparative analysis and validation studies have revealed that miR-455-3p was significantly decreased in muscle of both induced-atrophy model and old mice, whereas miR-434-3p was decreased in both serum and muscle of old mice, as compared to young mice. Furthermore, upregulation of miR-455-3p in fully differentiated C2C12 myoblasts induced a hypertrophic phenotype. These results suggest that deregulation of miR-455-3p may play a functional role in muscle atrophy and miR-434-3p could be a candidate serum biomarker of muscle aging.

KW - Disuse muscle atrophy

KW - MicroRNA

KW - Muscle aging

KW - Sarcopenia

KW - Serum

UR - http://www.scopus.com/inward/record.url?scp=85032623441&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85032623441&partnerID=8YFLogxK

U2 - 10.1093/gerona/glx025

DO - 10.1093/gerona/glx025

M3 - Article

VL - 72

SP - 1483

EP - 1491

JO - Journals of Gerontology - Series A Biological Sciences and Medical Sciences

JF - Journals of Gerontology - Series A Biological Sciences and Medical Sciences

SN - 1079-5006

IS - 11

ER -