Comprehensive miRNA Profiling of Skeletal Muscle and Serum in Induced and Normal Mouse Muscle Atrophy During Aging

Hwa Jin Jung, Kwang Pyo Lee, Brandon Milholland, Yeo Jin Shin, Jae Sook Kang, Ki Sun Kwon, Yousin Suh

Research output: Contribution to journalArticle

14 Scopus citations

Abstract

Age-associated loss of muscle mass and function is a major cause of morbidity and mortality in the elderly adults. Muscular atrophy can also be induced by disuse associated with long-term bed rest or disease. Although miRNAs regulate muscle growth, regeneration, and aging, their potential role in acute muscle atrophy is poorly understood. Furthermore, alterations in circulating miRNA levels have been shown to occur during aging but their potential as noninvasive biomarkers for muscle atrophy remains largely unexplored. Here, we report comprehensive miRNA expression profiles by miRNA-seq analysis in tibialis anterior muscle and serum of a disuse-induced atrophy mouse model, mimicking the acute atrophy following long-term bed rest, as compared to those of young and old mice. Comparative analysis and validation studies have revealed that miR-455-3p was significantly decreased in muscle of both induced-atrophy model and old mice, whereas miR-434-3p was decreased in both serum and muscle of old mice, as compared to young mice. Furthermore, upregulation of miR-455-3p in fully differentiated C2C12 myoblasts induced a hypertrophic phenotype. These results suggest that deregulation of miR-455-3p may play a functional role in muscle atrophy and miR-434-3p could be a candidate serum biomarker of muscle aging.

Original languageEnglish (US)
Pages (from-to)1483-1491
Number of pages9
JournalThe journals of gerontology. Series A, Biological sciences and medical sciences
Volume72
Issue number11
DOIs
StatePublished - Oct 12 2017

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Keywords

  • Disuse muscle atrophy
  • MicroRNA
  • Muscle aging
  • Sarcopenia
  • Serum

ASJC Scopus subject areas

  • Aging
  • Geriatrics and Gerontology

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