TY - JOUR
T1 - Composition of artificial tear solution affects in vitro pseudomonas aeruginosa biofilm formation on silicone hydrogel lens
AU - Yadav, Mukesh Kumar
AU - Chuck, Roy S.
AU - Park, Choul Yong
PY - 2013/7/1
Y1 - 2013/7/1
N2 - Purpose: To investigate Pseudomonas aeruginosa biofilm formation on silicone hydrogel contact lens in various artificial tears. Methods: P. aeruginosa was cocultured with contact lenses (Senofilcon A, Acuvue Oasys Hydraclear plus®; Johnson & Johnson, Jacksonville, FL) in artificial tears containing hyaluronic acid (HA) (0.3%, 0.1%, or 0.1%+benzalkonium chloride) or carboxymethylcellulose (0.5% or 0.3%+dextran). P. aeruginosa biofilm was stained with crystal violet (0.1%) after 48 h of incubation. The optical density of the dissolved biofilm was measured by a spectrophotometer at 570 nm and was compared. Results: P. aeruginosa biofilm formation in 3 solutions (0.3% HA, 0.1% HA+benzalkonium chloride and 0.3% carboxymethylcellulose+dextran) was significantly decreased compared to the other 2 solutions (0.1% HA and 0.5% carboxymethylcellulose) (P<0.01). Conclusions: P. aeruginosa biofilm formation on silicone hydrogel contact lens was affected by the composition of artificial tears. A higher concentration of HA and addition of benzalkonium chloride or dextran in artificial tears were helpful to prevent P. aeruginosa biofilm formation.
AB - Purpose: To investigate Pseudomonas aeruginosa biofilm formation on silicone hydrogel contact lens in various artificial tears. Methods: P. aeruginosa was cocultured with contact lenses (Senofilcon A, Acuvue Oasys Hydraclear plus®; Johnson & Johnson, Jacksonville, FL) in artificial tears containing hyaluronic acid (HA) (0.3%, 0.1%, or 0.1%+benzalkonium chloride) or carboxymethylcellulose (0.5% or 0.3%+dextran). P. aeruginosa biofilm was stained with crystal violet (0.1%) after 48 h of incubation. The optical density of the dissolved biofilm was measured by a spectrophotometer at 570 nm and was compared. Results: P. aeruginosa biofilm formation in 3 solutions (0.3% HA, 0.1% HA+benzalkonium chloride and 0.3% carboxymethylcellulose+dextran) was significantly decreased compared to the other 2 solutions (0.1% HA and 0.5% carboxymethylcellulose) (P<0.01). Conclusions: P. aeruginosa biofilm formation on silicone hydrogel contact lens was affected by the composition of artificial tears. A higher concentration of HA and addition of benzalkonium chloride or dextran in artificial tears were helpful to prevent P. aeruginosa biofilm formation.
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U2 - 10.1089/jop.2012.0078
DO - 10.1089/jop.2012.0078
M3 - Article
C2 - 23442006
AN - SCOPUS:84880216288
SN - 1080-7683
VL - 29
SP - 591
EP - 594
JO - Journal of Ocular Pharmacology and Therapeutics
JF - Journal of Ocular Pharmacology and Therapeutics
IS - 6
ER -