TY - JOUR
T1 - Complex N-glycans or core 1-derived O-glycans are not required for the expression of stage-specific antigens SSEA-1, SSEA-3, SSEA-4, or LeY in the preimplantation mouse embryo
AU - Williams, Suzannah A.
AU - Stanley, Pamela
N1 - Funding Information:
Acknowledgements We thank Wen Dong for excellent technical assistance and Dr. Jason Aglipay for help with some experiments. This work was supported by grant RO1 30645 from the National Institutes of Health to P. S. and received partial support from the Albert Einstein Cancer Center grant PO1 13330.
PY - 2009/4
Y1 - 2009/4
N2 - The glycan epitopes termed stage-specific embryonic antigens (SSEA) occur on glycoproteins and glycolipids in mammals. However, it is not known whether these epitopes are attached to N- or O-glycans on glycoproteins and/or on glycolipids in the developing mouse embryo. In this paper the expression of the antigens SSEA-1, SSEA-3, SSEA-4 and LeY was examined on ovulated eggs, early embryos and blastocysts lacking either complex and hybrid N-glycans or core-1 derived O-glycans. In all cases, antigen expression determined by fluorescence microscopy of bound monoclonal antibodies to embryos at the stage of development of maximal expression was similar in mutant and control embryos. Thus, none of these developmental antigens are expressed solely on either complex N- or core 1-derived O-glycans attached to glycoproteins in the preimplantation mouse embryo. Furthermore, neither of these classes of glycan is essential for the expression of SSEA-1, SSEA-3, SSEA-4 or LeY on mouse embryos.
AB - The glycan epitopes termed stage-specific embryonic antigens (SSEA) occur on glycoproteins and glycolipids in mammals. However, it is not known whether these epitopes are attached to N- or O-glycans on glycoproteins and/or on glycolipids in the developing mouse embryo. In this paper the expression of the antigens SSEA-1, SSEA-3, SSEA-4 and LeY was examined on ovulated eggs, early embryos and blastocysts lacking either complex and hybrid N-glycans or core-1 derived O-glycans. In all cases, antigen expression determined by fluorescence microscopy of bound monoclonal antibodies to embryos at the stage of development of maximal expression was similar in mutant and control embryos. Thus, none of these developmental antigens are expressed solely on either complex N- or core 1-derived O-glycans attached to glycoproteins in the preimplantation mouse embryo. Furthermore, neither of these classes of glycan is essential for the expression of SSEA-1, SSEA-3, SSEA-4 or LeY on mouse embryos.
KW - Immunofluorescence microscopy
KW - Mgat1
KW - N-glycans
KW - O-glycans
KW - Preimplantation mouse embryos
KW - SSEA
KW - T-synthase
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U2 - 10.1007/s10719-008-9181-1
DO - 10.1007/s10719-008-9181-1
M3 - Article
C2 - 18773292
AN - SCOPUS:62449334844
SN - 0282-0080
VL - 26
SP - 335
EP - 347
JO - Glycoconjugate Journal
JF - Glycoconjugate Journal
IS - 3
ER -