Comparison of toad bladder aldosterone-induced proteins and proteins synthesized in vitro using aldosterone-induced messenger RNA as template

Walter N. Scott, Irwin M. Reich, James A. Brown, Chia-Ping H. Yang

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Using double-labeled isotope techniques, it can be shown that aldosterone induces the synthesis of several proteins in the mitochondria-rich (MR) cells of the toad's urinary bladder. Induced proteins have been identified both in the plasma membrane (mol wt=170,000, 85,000 and 12,000) and the cytosol (mol wt=36,000, 12,000 and 6,000) fractions of these mucosal cells. We have also shown that aldosterone (Aldo) induces the synthesis of a class of RNA having the properties of messenger RNA (mRNA). mRNA isolated from Aldo-treated mucosal cells was used as template in a cell-free protein-synthesis system prepared from rabbit reticulocytes. Preparations charged with mRNA from Aldotreated cells synthesized two proteins that were not labeled when mRNA from control tissues was used as template. The electrophoretic mobility of one of these proteins was similar to an Aldo-induced membrane protein (mol wt=70,000) found in the intact tissue.

Original languageEnglish (US)
Pages (from-to)213-220
Number of pages8
JournalThe Journal of Membrane Biology
Volume40
Issue number1 Supplement
DOIs
StatePublished - Mar 1978
Externally publishedYes

Fingerprint

Aldosterone
Anura
Urinary Bladder
Messenger RNA
Proteins
Reticulocytes
Isotopes
Cytosol
Mitochondria
Membrane Proteins
Cell Membrane
In Vitro Techniques
RNA
Rabbits

ASJC Scopus subject areas

  • Physiology
  • Cell Biology
  • Biophysics

Cite this

Comparison of toad bladder aldosterone-induced proteins and proteins synthesized in vitro using aldosterone-induced messenger RNA as template. / Scott, Walter N.; Reich, Irwin M.; Brown, James A.; Yang, Chia-Ping H.

In: The Journal of Membrane Biology, Vol. 40, No. 1 Supplement, 03.1978, p. 213-220.

Research output: Contribution to journalArticle

@article{f6039acbcd354818a27fe61ecf334819,
title = "Comparison of toad bladder aldosterone-induced proteins and proteins synthesized in vitro using aldosterone-induced messenger RNA as template",
abstract = "Using double-labeled isotope techniques, it can be shown that aldosterone induces the synthesis of several proteins in the mitochondria-rich (MR) cells of the toad's urinary bladder. Induced proteins have been identified both in the plasma membrane (mol wt=170,000, 85,000 and 12,000) and the cytosol (mol wt=36,000, 12,000 and 6,000) fractions of these mucosal cells. We have also shown that aldosterone (Aldo) induces the synthesis of a class of RNA having the properties of messenger RNA (mRNA). mRNA isolated from Aldo-treated mucosal cells was used as template in a cell-free protein-synthesis system prepared from rabbit reticulocytes. Preparations charged with mRNA from Aldotreated cells synthesized two proteins that were not labeled when mRNA from control tissues was used as template. The electrophoretic mobility of one of these proteins was similar to an Aldo-induced membrane protein (mol wt=70,000) found in the intact tissue.",
author = "Scott, {Walter N.} and Reich, {Irwin M.} and Brown, {James A.} and Yang, {Chia-Ping H.}",
year = "1978",
month = "3",
doi = "10.1007/BF02026007",
language = "English (US)",
volume = "40",
pages = "213--220",
journal = "Journal of Membrane Biology",
issn = "0022-2631",
publisher = "Springer New York",
number = "1 Supplement",

}

TY - JOUR

T1 - Comparison of toad bladder aldosterone-induced proteins and proteins synthesized in vitro using aldosterone-induced messenger RNA as template

AU - Scott, Walter N.

AU - Reich, Irwin M.

AU - Brown, James A.

AU - Yang, Chia-Ping H.

PY - 1978/3

Y1 - 1978/3

N2 - Using double-labeled isotope techniques, it can be shown that aldosterone induces the synthesis of several proteins in the mitochondria-rich (MR) cells of the toad's urinary bladder. Induced proteins have been identified both in the plasma membrane (mol wt=170,000, 85,000 and 12,000) and the cytosol (mol wt=36,000, 12,000 and 6,000) fractions of these mucosal cells. We have also shown that aldosterone (Aldo) induces the synthesis of a class of RNA having the properties of messenger RNA (mRNA). mRNA isolated from Aldo-treated mucosal cells was used as template in a cell-free protein-synthesis system prepared from rabbit reticulocytes. Preparations charged with mRNA from Aldotreated cells synthesized two proteins that were not labeled when mRNA from control tissues was used as template. The electrophoretic mobility of one of these proteins was similar to an Aldo-induced membrane protein (mol wt=70,000) found in the intact tissue.

AB - Using double-labeled isotope techniques, it can be shown that aldosterone induces the synthesis of several proteins in the mitochondria-rich (MR) cells of the toad's urinary bladder. Induced proteins have been identified both in the plasma membrane (mol wt=170,000, 85,000 and 12,000) and the cytosol (mol wt=36,000, 12,000 and 6,000) fractions of these mucosal cells. We have also shown that aldosterone (Aldo) induces the synthesis of a class of RNA having the properties of messenger RNA (mRNA). mRNA isolated from Aldo-treated mucosal cells was used as template in a cell-free protein-synthesis system prepared from rabbit reticulocytes. Preparations charged with mRNA from Aldotreated cells synthesized two proteins that were not labeled when mRNA from control tissues was used as template. The electrophoretic mobility of one of these proteins was similar to an Aldo-induced membrane protein (mol wt=70,000) found in the intact tissue.

UR - http://www.scopus.com/inward/record.url?scp=0018135190&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0018135190&partnerID=8YFLogxK

U2 - 10.1007/BF02026007

DO - 10.1007/BF02026007

M3 - Article

C2 - 731677

AN - SCOPUS:0018135190

VL - 40

SP - 213

EP - 220

JO - Journal of Membrane Biology

JF - Journal of Membrane Biology

SN - 0022-2631

IS - 1 Supplement

ER -