Comparison of the spectroscopic and saccharide binding properties of lentil and pea isolectins

Lokesh Bhattacharyya, Jonathan H. Freedman, Curtis F. Brewer, Rodney D. Brown, Seymour H. Koenig

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

The lentil isolectins, CMLcH A and CMLcH B, and pea isolectins, CMPSA A and CMPSA B, are compared in terms of their spectroscopic and saccharide binding properties. The paramagnetic contribution to the solvent proton magnetic relaxation dispersion profiles of solutions of the isolectins of each protein are found to be essentially identical. Electron paramagnetic resonance spectra suggest a high degree of octahedral symmetry at the Mn2+ site for both pairs of isolectins. The near-ultraviolet absorption spectra of CMLcH A and CMLcH B are identical, as are the spectra of CMPSA A and CMPSA B. Carbohydrate binding activities of the isolectins of each protein are compared using hemagglutination, precipitation, and precipitation-inhibition assays, and are found to be identical, although the activities of CMLcH and CMPSA differ somewhat. These results demonstrate that the spectroscopic and saccharide binding properties of the isolectins of CMLcH are essentially identical, as are those of the isolectins of CMPSA, and suggest that native mixtures of the isolectins may be treated as single proteins in further studies.

Original languageEnglish (US)
Pages (from-to)820-826
Number of pages7
JournalArchives of Biochemistry and Biophysics
Volume240
Issue number2
DOIs
StatePublished - Aug 1 1985

Fingerprint

Lens Plant
Peas
Lectins
Magnetic relaxation
Proteins
Electron Spin Resonance Spectroscopy
Hemagglutination
Paramagnetic resonance
Protons
Absorption spectra
Assays
Carbohydrates

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Comparison of the spectroscopic and saccharide binding properties of lentil and pea isolectins. / Bhattacharyya, Lokesh; Freedman, Jonathan H.; Brewer, Curtis F.; Brown, Rodney D.; Koenig, Seymour H.

In: Archives of Biochemistry and Biophysics, Vol. 240, No. 2, 01.08.1985, p. 820-826.

Research output: Contribution to journalArticle

Bhattacharyya, Lokesh ; Freedman, Jonathan H. ; Brewer, Curtis F. ; Brown, Rodney D. ; Koenig, Seymour H. / Comparison of the spectroscopic and saccharide binding properties of lentil and pea isolectins. In: Archives of Biochemistry and Biophysics. 1985 ; Vol. 240, No. 2. pp. 820-826.
@article{aea319f794c746ed8bb13ddb3fd5f174,
title = "Comparison of the spectroscopic and saccharide binding properties of lentil and pea isolectins",
abstract = "The lentil isolectins, CMLcH A and CMLcH B, and pea isolectins, CMPSA A and CMPSA B, are compared in terms of their spectroscopic and saccharide binding properties. The paramagnetic contribution to the solvent proton magnetic relaxation dispersion profiles of solutions of the isolectins of each protein are found to be essentially identical. Electron paramagnetic resonance spectra suggest a high degree of octahedral symmetry at the Mn2+ site for both pairs of isolectins. The near-ultraviolet absorption spectra of CMLcH A and CMLcH B are identical, as are the spectra of CMPSA A and CMPSA B. Carbohydrate binding activities of the isolectins of each protein are compared using hemagglutination, precipitation, and precipitation-inhibition assays, and are found to be identical, although the activities of CMLcH and CMPSA differ somewhat. These results demonstrate that the spectroscopic and saccharide binding properties of the isolectins of CMLcH are essentially identical, as are those of the isolectins of CMPSA, and suggest that native mixtures of the isolectins may be treated as single proteins in further studies.",
author = "Lokesh Bhattacharyya and Freedman, {Jonathan H.} and Brewer, {Curtis F.} and Brown, {Rodney D.} and Koenig, {Seymour H.}",
year = "1985",
month = "8",
day = "1",
doi = "10.1016/0003-9861(85)90091-8",
language = "English (US)",
volume = "240",
pages = "820--826",
journal = "Archives of Biochemistry and Biophysics",
issn = "0003-9861",
publisher = "Academic Press Inc.",
number = "2",

}

TY - JOUR

T1 - Comparison of the spectroscopic and saccharide binding properties of lentil and pea isolectins

AU - Bhattacharyya, Lokesh

AU - Freedman, Jonathan H.

AU - Brewer, Curtis F.

AU - Brown, Rodney D.

AU - Koenig, Seymour H.

PY - 1985/8/1

Y1 - 1985/8/1

N2 - The lentil isolectins, CMLcH A and CMLcH B, and pea isolectins, CMPSA A and CMPSA B, are compared in terms of their spectroscopic and saccharide binding properties. The paramagnetic contribution to the solvent proton magnetic relaxation dispersion profiles of solutions of the isolectins of each protein are found to be essentially identical. Electron paramagnetic resonance spectra suggest a high degree of octahedral symmetry at the Mn2+ site for both pairs of isolectins. The near-ultraviolet absorption spectra of CMLcH A and CMLcH B are identical, as are the spectra of CMPSA A and CMPSA B. Carbohydrate binding activities of the isolectins of each protein are compared using hemagglutination, precipitation, and precipitation-inhibition assays, and are found to be identical, although the activities of CMLcH and CMPSA differ somewhat. These results demonstrate that the spectroscopic and saccharide binding properties of the isolectins of CMLcH are essentially identical, as are those of the isolectins of CMPSA, and suggest that native mixtures of the isolectins may be treated as single proteins in further studies.

AB - The lentil isolectins, CMLcH A and CMLcH B, and pea isolectins, CMPSA A and CMPSA B, are compared in terms of their spectroscopic and saccharide binding properties. The paramagnetic contribution to the solvent proton magnetic relaxation dispersion profiles of solutions of the isolectins of each protein are found to be essentially identical. Electron paramagnetic resonance spectra suggest a high degree of octahedral symmetry at the Mn2+ site for both pairs of isolectins. The near-ultraviolet absorption spectra of CMLcH A and CMLcH B are identical, as are the spectra of CMPSA A and CMPSA B. Carbohydrate binding activities of the isolectins of each protein are compared using hemagglutination, precipitation, and precipitation-inhibition assays, and are found to be identical, although the activities of CMLcH and CMPSA differ somewhat. These results demonstrate that the spectroscopic and saccharide binding properties of the isolectins of CMLcH are essentially identical, as are those of the isolectins of CMPSA, and suggest that native mixtures of the isolectins may be treated as single proteins in further studies.

UR - http://www.scopus.com/inward/record.url?scp=0022260878&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0022260878&partnerID=8YFLogxK

U2 - 10.1016/0003-9861(85)90091-8

DO - 10.1016/0003-9861(85)90091-8

M3 - Article

C2 - 2992383

AN - SCOPUS:0022260878

VL - 240

SP - 820

EP - 826

JO - Archives of Biochemistry and Biophysics

JF - Archives of Biochemistry and Biophysics

SN - 0003-9861

IS - 2

ER -