Comparison of the late HI histone genes of the sea urchins Lytechinus pictus and Strongelocentrotus purpuratus

James A. Knowles, Geoffrey J. Childs

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

We have isolated and sequenced a gene encoding a late H1 histone subtype from the sea urchin species L. pictus. The primary structure of the late H1 subtype encoded by this gene is 209 amino acids in length, and has a net positive charge of 67. This gene is present in a single copy per haploid genome and encodes an mRNA of 752 nucleotldes. Late H1 transcripts are detected in the imfertilixed egg and are most prevalent in gastrulating embryos. Comparison of 375 bp of 5′ flanking sequences of the L. pictus late H1 gene and the H1-gamma gene of a distantly related sea urchin species, S. purpuratus, reveals large blocks of sequences that are identical between the two genes. To determine if these conserved 5′ sequences are present in other members of the sea urchin H1 gene family, the analogous region of S. purpuratus H1-alpha, an early H1 gene, was sequenced. The homology between the flanking sequences of the early and late families was Halted to consensus sequences which are found upstream of all H1 genes. The possible regulatory implications of these findings are discussed.

Original languageEnglish (US)
Pages (from-to)8121-8133
Number of pages13
JournalNucleic Acids Research
Volume14
Issue number20
DOIs
StatePublished - Oct 1986

Fingerprint

Lytechinus
Sea Urchins
Histones
Genes
Gene
Gene encoding
Conserved Sequence
5' Flanking Region
Haploidy
Consensus Sequence
Embryo
Messenger RNA
Ovum
Amino Acids
Amino acids
Homology
Genome
Encoding
Embryonic Structures
Charge

ASJC Scopus subject areas

  • Statistics, Probability and Uncertainty
  • Applied Mathematics
  • Health, Toxicology and Mutagenesis
  • Toxicology
  • Genetics(clinical)
  • Genetics

Cite this

Comparison of the late HI histone genes of the sea urchins Lytechinus pictus and Strongelocentrotus purpuratus. / Knowles, James A.; Childs, Geoffrey J.

In: Nucleic Acids Research, Vol. 14, No. 20, 10.1986, p. 8121-8133.

Research output: Contribution to journalArticle

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