TY - JOUR
T1 - Comparison of the late HI histone genes of the sea urchins Lytechinus pictus and Strongelocentrotus purpuratus
AU - Knowles, James A.
AU - Childs, Geoffrey J.
N1 - Funding Information:
ACKNOWLEDGEMENTS We would like to thank Eric Davidson for providing the EcoRI genomlc library of L_. plctus DNA and to KenKrauter for helpful comments. This work was supported by grant #GM30333 from the NIH. Jim Knowles Is supported by a predoctoral M.D./Ph.D. training grant NIHgrant # T32GH7288. Computer resources provided by the BIONET National Computer Resource for Molecular Biology whose funding Is provided by NIHgrant f 1U41RR-01685.
PY - 1986/10
Y1 - 1986/10
N2 - We have isolated and sequenced a gene encoding a late H1 histone subtype from the sea urchin species L. pictus. The primary structure of the late H1 subtype encoded by this gene is 209 amino acids in length, and has a net positive charge of 67. This gene is present in a single copy per haploid genome and encodes an mRNA of 752 nucleotldes. Late H1 transcripts are detected in the imfertilixed egg and are most prevalent in gastrulating embryos. Comparison of 375 bp of 5′ flanking sequences of the L. pictus late H1 gene and the H1-gamma gene of a distantly related sea urchin species, S. purpuratus, reveals large blocks of sequences that are identical between the two genes. To determine if these conserved 5′ sequences are present in other members of the sea urchin H1 gene family, the analogous region of S. purpuratus H1-alpha, an early H1 gene, was sequenced. The homology between the flanking sequences of the early and late families was Halted to consensus sequences which are found upstream of all H1 genes. The possible regulatory implications of these findings are discussed.
AB - We have isolated and sequenced a gene encoding a late H1 histone subtype from the sea urchin species L. pictus. The primary structure of the late H1 subtype encoded by this gene is 209 amino acids in length, and has a net positive charge of 67. This gene is present in a single copy per haploid genome and encodes an mRNA of 752 nucleotldes. Late H1 transcripts are detected in the imfertilixed egg and are most prevalent in gastrulating embryos. Comparison of 375 bp of 5′ flanking sequences of the L. pictus late H1 gene and the H1-gamma gene of a distantly related sea urchin species, S. purpuratus, reveals large blocks of sequences that are identical between the two genes. To determine if these conserved 5′ sequences are present in other members of the sea urchin H1 gene family, the analogous region of S. purpuratus H1-alpha, an early H1 gene, was sequenced. The homology between the flanking sequences of the early and late families was Halted to consensus sequences which are found upstream of all H1 genes. The possible regulatory implications of these findings are discussed.
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U2 - 10.1093/nar/14.20.8121
DO - 10.1093/nar/14.20.8121
M3 - Article
C2 - 3022245
AN - SCOPUS:0023056208
SN - 0305-1048
VL - 14
SP - 8121
EP - 8133
JO - Nucleic acids research
JF - Nucleic acids research
IS - 20
ER -