Comparison of relative changes in phosphatic metabolites and phospholipids after irradiation

T. E. Merchant, A. A. Alfieri, T. Glonek, J. A. Koutcher

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Measurements of phosphatic metabolites and phospholipids using 31P NMR spectroscopy were performed on tumor extracts at specific times before and after a priming dose of radiation at 32 or 65 Gy. At both doses, statistically significant increases in the high-energy phosphates PCr and ATP, relative to their primary hydrolysis product Pi, were detected at 24 h after irradiation. These spectral changes are consistent with an increased capacity for aerobic metabolism and oxidative phosphorylation. The changes coincide with a significant reduction in the hypoxic cell fraction and 50% tumor control dose measurements reported previously for the same tumor model. A significant increase in the ratio of phosphorylethanolamine to phosphorylcholine was also detected at 24 h. The ratio of ethanolamine- containing to choline-containing phospholipids increased significantly between 48 and 96 h. The spectral changes measured in this study coincide with the changes in the hypoxic state of the tumor after irradiation and are consistent with spectral measurements performed in vivo. The high-resolution spectroscopic data also identify specific metabolites contributing to spectral changes measured in vivo.

Original languageEnglish (US)
Pages (from-to)29-38
Number of pages10
JournalRadiation Research
Volume142
Issue number1
DOIs
StatePublished - 1995
Externally publishedYes

Fingerprint

metabolites
Phospholipids
phospholipids
tumors
irradiation
neoplasms
dosage
Neoplasms
priming
choline
phosphorylation
Ethanolamine
ethanolamine
Phosphorylcholine
oxidative phosphorylation
adenosine triphosphate
Oxidative Phosphorylation
metabolism
Choline
hydrolysis

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Radiology Nuclear Medicine and imaging
  • Biophysics
  • Radiation

Cite this

Comparison of relative changes in phosphatic metabolites and phospholipids after irradiation. / Merchant, T. E.; Alfieri, A. A.; Glonek, T.; Koutcher, J. A.

In: Radiation Research, Vol. 142, No. 1, 1995, p. 29-38.

Research output: Contribution to journalArticle

Merchant, T. E. ; Alfieri, A. A. ; Glonek, T. ; Koutcher, J. A. / Comparison of relative changes in phosphatic metabolites and phospholipids after irradiation. In: Radiation Research. 1995 ; Vol. 142, No. 1. pp. 29-38.
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