Comparison of rapid diagnostic methodologies for chlamydia and gonorrhea in an urban adolescent population: A pilot study

D. K. Bryant, A. S. Fox, I. Spigland, E. Childers, M. Motyl, W. D. Rosenfeld

Research output: Contribution to journalComment/debate

7 Scopus citations

Abstract

Purpose: To compare a combination DNA probe test which detects both N. gonorrhoeae (GC) and C. trachomatis (CT) to the current culture methodologies among a population of female adolescents at an urban teaching center. In addition, the probe test for CT was compared to a direct immunofluorescence test. Methods: All sexually active female adolescents between the ages of 13-21 years who sought care at an urban teaching center from June 1991 through November 1991 and who required testing for sexually transmitted diseases (STDs) were recruited for this study. Results: The probe test was demonstrated to be 66.6% sensitive and 94.9% specific when compared to tissue culture for CT and 50% sensitive and 98.2% specific when compared to culture for GC. We found an overall prevalence of 23.5% for CT and 3.5% for GC. Conclusions: The two rapid diagnostic tests for CT evaluated in this study demonstrated similar sensitivities. However the probe test offers advantages in that it is easier to perform, skill at reading fluorescence is not required, and one specimen yields results for both CT and GC.

Original languageEnglish (US)
Pages (from-to)324-327
Number of pages4
JournalJournal of Adolescent Health
Volume16
Issue number4
DOIs
StatePublished - Apr 1995
Externally publishedYes

Keywords

  • C. trachomatis
  • DNA probe test N. gonorrhoeae
  • Sexually transmitted diseases
  • Tissue culture test

ASJC Scopus subject areas

  • Pediatrics, Perinatology, and Child Health
  • Public Health, Environmental and Occupational Health
  • Psychiatry and Mental health

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