Comparison of BIOMED-2 versus laboratory-developed polymerase chain reaction assays for detecting T-cell receptor-γ gene rearrangements

Keyur P. Patel, Qiulu Pan, Yanhua Wang, Robert W. Maitta, Juan Du, Xiaonan Xue, Juan Lin, Howard Ratech

Research output: Contribution to journalArticle

26 Scopus citations

Abstract

Detecting clonal T-cell receptor (TCR)-γ gene rearrangements (GRs) is an important adjunct test for diagnosing T-cell lymphoma. We compared a recently described assay (BIOMED-2 protocol), which targets multiple variable (V) gene segments in two polymerase chain reaction (PCR) reactions (multi-V), with a frequently referenced assay that targets a single V gene segment in four separate PCR reactions (mono-V). A total of 144 consecutive clinical DNA samples were prospectively tested for T-cell clonality by PCR using laboratory-developed mono-V and commercial multi-V primer sets for TCR-γ GR. The combination of TCR-β, mono-V TCR-γ and multi-V TCR-γ detected more clonal cases (68/144, 47%) than any individual PCR assay. We detected clonal TCR-β GR in 47/68 (69%) cases. Using either mono-V or multi-V TCR-γ primers, the sensitivities for detecting clonality were 52/68 (76%) or 51/68 (75%). Using both mono-V and multi-V TCR-γ primers improved the sensitivity for detecting clonality, 60/68 (88%). Combining either mono-V or multi-V TCR-γ primers with TCR-β primers also improved the sensitivity, 64/68 (94%). Significantly, TCR-γ V11 GRs could only be detected using the mono-V-PCR primers. We conclude that using more than one T-cell PCR assay can enhance the overall sensitivity for detecting T-cell clonality.

Original languageEnglish (US)
Pages (from-to)226-237
Number of pages12
JournalJournal of Molecular Diagnostics
Volume12
Issue number2
DOIs
StatePublished - Mar 2010

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ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Molecular Medicine

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