Comparison of an anti-HSV-2 monoclonal IgG and its IgA switch variant for topical immunoprotection of the mouse vagina

L. Zeitlin; P. E. Castle; K. J. Whaley; T. R. Moench; R. A. Cone

doi:10.1016/S0165-0378(98)00041-2

Comparison of an anti-HSV-2 monoclonal IgG and its IgA switch variant for topical immunoprotection of the mouse vagina

L. Zeitlin, P. E. Castle, K. J. Whaley, T. R. Moench, R. A. Cone

Research output: Contribution to journal › Article › peer-review

12 Scopus citations

Abstract

An IgG(2a) monoclonal antibody (Mab) directed against glycoprotein D of herpes simplex virus 2 (HSV-2) was compared with an IgA heavy chain Mab switch variant to investigate the effect of isotype for topical immunoprotection of the murine vagina. The IgA Mab, a mixture of monomeric and polymeric IgA, was indistinguishable from its IgG parent in an in vitro HSV-2 neutralization assay. When these class switched Mabs were delivered to the mouse vagina, we also found no significant difference between the IgG and IgA for preventing vaginal transmission of HSV-2 infection. The implications of these results for active and passive immunization strategies against vaginal transmission of genital herpes infections are discussed.

Original language	English (US)
Pages (from-to)	93-101
Number of pages	9
Journal	Journal of Reproductive Immunology
Volume	40
Issue number	1
DOIs	https://doi.org/10.1016/S0165-0378(98)00041-2
State	Published - Oct 1998
Externally published	Yes

Keywords

Anti-HSV-2 monoclonal IgG
Genital herpes
Immunoprotection

ASJC Scopus subject areas

Immunology and Allergy
Immunology
Reproductive Medicine
Obstetrics and Gynecology

Access to Document

10.1016/S0165-0378(98)00041-2

Cite this

@article{3201c293c4254b33a90bc1b87bd65eff,

title = "Comparison of an anti-HSV-2 monoclonal IgG and its IgA switch variant for topical immunoprotection of the mouse vagina",

abstract = "An IgG(2a) monoclonal antibody (Mab) directed against glycoprotein D of herpes simplex virus 2 (HSV-2) was compared with an IgA heavy chain Mab switch variant to investigate the effect of isotype for topical immunoprotection of the murine vagina. The IgA Mab, a mixture of monomeric and polymeric IgA, was indistinguishable from its IgG parent in an in vitro HSV-2 neutralization assay. When these class switched Mabs were delivered to the mouse vagina, we also found no significant difference between the IgG and IgA for preventing vaginal transmission of HSV-2 infection. The implications of these results for active and passive immunization strategies against vaginal transmission of genital herpes infections are discussed.",

keywords = "Anti-HSV-2 monoclonal IgG, Genital herpes, Immunoprotection",

author = "L. Zeitlin and Castle, {P. E.} and Whaley, {K. J.} and Moench, {T. R.} and Cone, {R. A.}",

year = "1998",

month = oct,

doi = "10.1016/S0165-0378(98)00041-2",

language = "English (US)",

volume = "40",

pages = "93--101",

journal = "Journal of Reproductive Immunology",

issn = "0165-0378",

publisher = "Elsevier Ireland Ltd",

number = "1",

}

TY - JOUR

T1 - Comparison of an anti-HSV-2 monoclonal IgG and its IgA switch variant for topical immunoprotection of the mouse vagina

AU - Zeitlin, L.

AU - Castle, P. E.

AU - Whaley, K. J.

AU - Moench, T. R.

AU - Cone, R. A.

PY - 1998/10

Y1 - 1998/10

N2 - An IgG(2a) monoclonal antibody (Mab) directed against glycoprotein D of herpes simplex virus 2 (HSV-2) was compared with an IgA heavy chain Mab switch variant to investigate the effect of isotype for topical immunoprotection of the murine vagina. The IgA Mab, a mixture of monomeric and polymeric IgA, was indistinguishable from its IgG parent in an in vitro HSV-2 neutralization assay. When these class switched Mabs were delivered to the mouse vagina, we also found no significant difference between the IgG and IgA for preventing vaginal transmission of HSV-2 infection. The implications of these results for active and passive immunization strategies against vaginal transmission of genital herpes infections are discussed.

AB - An IgG(2a) monoclonal antibody (Mab) directed against glycoprotein D of herpes simplex virus 2 (HSV-2) was compared with an IgA heavy chain Mab switch variant to investigate the effect of isotype for topical immunoprotection of the murine vagina. The IgA Mab, a mixture of monomeric and polymeric IgA, was indistinguishable from its IgG parent in an in vitro HSV-2 neutralization assay. When these class switched Mabs were delivered to the mouse vagina, we also found no significant difference between the IgG and IgA for preventing vaginal transmission of HSV-2 infection. The implications of these results for active and passive immunization strategies against vaginal transmission of genital herpes infections are discussed.

KW - Anti-HSV-2 monoclonal IgG

KW - Genital herpes

KW - Immunoprotection

UR - http://www.scopus.com/inward/record.url?scp=0032432744&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0032432744&partnerID=8YFLogxK

U2 - 10.1016/S0165-0378(98)00041-2

DO - 10.1016/S0165-0378(98)00041-2

M3 - Article

C2 - 9862259

AN - SCOPUS:0032432744

SN - 0165-0378

VL - 40

SP - 93

EP - 101

JO - Journal of Reproductive Immunology

JF - Journal of Reproductive Immunology

IS - 1

ER -

Comparison of an anti-HSV-2 monoclonal IgG and its IgA switch variant for topical immunoprotection of the mouse vagina

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this