Comparison of an anti-HSV-2 monoclonal IgG and its IgA switch variant for topical immunoprotection of the mouse vagina

L. Zeitlin, P. E. Castle, K. J. Whaley, T. R. Moench, R. A. Cone

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12 Scopus citations

Abstract

An IgG(2a) monoclonal antibody (Mab) directed against glycoprotein D of herpes simplex virus 2 (HSV-2) was compared with an IgA heavy chain Mab switch variant to investigate the effect of isotype for topical immunoprotection of the murine vagina. The IgA Mab, a mixture of monomeric and polymeric IgA, was indistinguishable from its IgG parent in an in vitro HSV-2 neutralization assay. When these class switched Mabs were delivered to the mouse vagina, we also found no significant difference between the IgG and IgA for preventing vaginal transmission of HSV-2 infection. The implications of these results for active and passive immunization strategies against vaginal transmission of genital herpes infections are discussed.

Original languageEnglish (US)
Pages (from-to)93-101
Number of pages9
JournalJournal of Reproductive Immunology
Volume40
Issue number1
DOIs
StatePublished - Oct 1 1998
Externally publishedYes

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Keywords

  • Anti-HSV-2 monoclonal IgG
  • Genital herpes
  • Immunoprotection

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Reproductive Medicine
  • Obstetrics and Gynecology

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