Comparative study of laser and scalpel nerve transections

I. Fligny, J. S. Wu, B. R. Samonte, Marvin P. Fried

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

This investigation was designed to compare standard scalpel transections of the tibial branch of the rat sciatic nerve with those performed using either a milliwatt carbon dioxide (CO2) or a potassium titanyl phosphate (KTP/532) laser. Four transection groups consisted of nerves sectioned with (1) scalpel (control), (2) milliwatt CO2 laser, (3) KTP/532 with microscope attachment, and (4) KTP/532 laser with 400-μm bare fiber. Each laser was used with the same parameters: 10 watts, 0.4-mm spot size, and continuous- wave mode. Horseradish peroxidase (HRP) was applied to the proximal stump for 30 min, and the animals were sacrificed 24 h later. Horseradish peroxidase (HRP)-labeled motoneuron cell bodies in the lumbar spinal cord were then counted. The average numbers of labeled neurons in each group were as follows: group I (n = 14) 518, group II (n = 8) 424, group III (n = 8) 351, and group IV (n = 8) 283. The standard deviations were quite large, however. When all laser transections were pooled and compared with paired scalpel transections, we found a significant difference, both by the paired t-test (P = 0.016) and by the Wilcoxon matched-paired test (P = 0.02). We conclude that laser transection significantly diminishes the number of neurons labeled by the retrograde transport of HRP.

Original languageEnglish (US)
Pages (from-to)43-50
Number of pages8
JournalLasers in Surgery and Medicine
Volume12
Issue number1
StatePublished - 1992
Externally publishedYes

Fingerprint

Laser Therapy
Horseradish Peroxidase
Lasers
Solid-State Lasers
Spinal Cord
Neurons
Gas Lasers
Motor Neurons
Sciatic Nerve
Carbon Dioxide

ASJC Scopus subject areas

  • Surgery

Cite this

Comparative study of laser and scalpel nerve transections. / Fligny, I.; Wu, J. S.; Samonte, B. R.; Fried, Marvin P.

In: Lasers in Surgery and Medicine, Vol. 12, No. 1, 1992, p. 43-50.

Research output: Contribution to journalArticle

Fligny, I, Wu, JS, Samonte, BR & Fried, MP 1992, 'Comparative study of laser and scalpel nerve transections', Lasers in Surgery and Medicine, vol. 12, no. 1, pp. 43-50.
Fligny, I. ; Wu, J. S. ; Samonte, B. R. ; Fried, Marvin P. / Comparative study of laser and scalpel nerve transections. In: Lasers in Surgery and Medicine. 1992 ; Vol. 12, No. 1. pp. 43-50.
@article{b51fda286280484fb3c853eb8087447d,
title = "Comparative study of laser and scalpel nerve transections",
abstract = "This investigation was designed to compare standard scalpel transections of the tibial branch of the rat sciatic nerve with those performed using either a milliwatt carbon dioxide (CO2) or a potassium titanyl phosphate (KTP/532) laser. Four transection groups consisted of nerves sectioned with (1) scalpel (control), (2) milliwatt CO2 laser, (3) KTP/532 with microscope attachment, and (4) KTP/532 laser with 400-μm bare fiber. Each laser was used with the same parameters: 10 watts, 0.4-mm spot size, and continuous- wave mode. Horseradish peroxidase (HRP) was applied to the proximal stump for 30 min, and the animals were sacrificed 24 h later. Horseradish peroxidase (HRP)-labeled motoneuron cell bodies in the lumbar spinal cord were then counted. The average numbers of labeled neurons in each group were as follows: group I (n = 14) 518, group II (n = 8) 424, group III (n = 8) 351, and group IV (n = 8) 283. The standard deviations were quite large, however. When all laser transections were pooled and compared with paired scalpel transections, we found a significant difference, both by the paired t-test (P = 0.016) and by the Wilcoxon matched-paired test (P = 0.02). We conclude that laser transection significantly diminishes the number of neurons labeled by the retrograde transport of HRP.",
author = "I. Fligny and Wu, {J. S.} and Samonte, {B. R.} and Fried, {Marvin P.}",
year = "1992",
language = "English (US)",
volume = "12",
pages = "43--50",
journal = "Lasers in Surgery and Medicine",
issn = "0196-8092",
publisher = "Wiley-Liss Inc.",
number = "1",

}

TY - JOUR

T1 - Comparative study of laser and scalpel nerve transections

AU - Fligny, I.

AU - Wu, J. S.

AU - Samonte, B. R.

AU - Fried, Marvin P.

PY - 1992

Y1 - 1992

N2 - This investigation was designed to compare standard scalpel transections of the tibial branch of the rat sciatic nerve with those performed using either a milliwatt carbon dioxide (CO2) or a potassium titanyl phosphate (KTP/532) laser. Four transection groups consisted of nerves sectioned with (1) scalpel (control), (2) milliwatt CO2 laser, (3) KTP/532 with microscope attachment, and (4) KTP/532 laser with 400-μm bare fiber. Each laser was used with the same parameters: 10 watts, 0.4-mm spot size, and continuous- wave mode. Horseradish peroxidase (HRP) was applied to the proximal stump for 30 min, and the animals were sacrificed 24 h later. Horseradish peroxidase (HRP)-labeled motoneuron cell bodies in the lumbar spinal cord were then counted. The average numbers of labeled neurons in each group were as follows: group I (n = 14) 518, group II (n = 8) 424, group III (n = 8) 351, and group IV (n = 8) 283. The standard deviations were quite large, however. When all laser transections were pooled and compared with paired scalpel transections, we found a significant difference, both by the paired t-test (P = 0.016) and by the Wilcoxon matched-paired test (P = 0.02). We conclude that laser transection significantly diminishes the number of neurons labeled by the retrograde transport of HRP.

AB - This investigation was designed to compare standard scalpel transections of the tibial branch of the rat sciatic nerve with those performed using either a milliwatt carbon dioxide (CO2) or a potassium titanyl phosphate (KTP/532) laser. Four transection groups consisted of nerves sectioned with (1) scalpel (control), (2) milliwatt CO2 laser, (3) KTP/532 with microscope attachment, and (4) KTP/532 laser with 400-μm bare fiber. Each laser was used with the same parameters: 10 watts, 0.4-mm spot size, and continuous- wave mode. Horseradish peroxidase (HRP) was applied to the proximal stump for 30 min, and the animals were sacrificed 24 h later. Horseradish peroxidase (HRP)-labeled motoneuron cell bodies in the lumbar spinal cord were then counted. The average numbers of labeled neurons in each group were as follows: group I (n = 14) 518, group II (n = 8) 424, group III (n = 8) 351, and group IV (n = 8) 283. The standard deviations were quite large, however. When all laser transections were pooled and compared with paired scalpel transections, we found a significant difference, both by the paired t-test (P = 0.016) and by the Wilcoxon matched-paired test (P = 0.02). We conclude that laser transection significantly diminishes the number of neurons labeled by the retrograde transport of HRP.

UR - http://www.scopus.com/inward/record.url?scp=0026582473&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0026582473&partnerID=8YFLogxK

M3 - Article

VL - 12

SP - 43

EP - 50

JO - Lasers in Surgery and Medicine

JF - Lasers in Surgery and Medicine

SN - 0196-8092

IS - 1

ER -