Abstract
This investigation was designed to compare standard scalpel transections of the tibial branch of the rat sciatic nerve with those performed using either a milliwatt carbon dioxide (CO2) or a potassium titanyl phosphate (KTP/532) laser. Four transection groups consisted of nerves sectioned with (1) scalpel (control), (2) milliwatt CO2 laser, (3) KTP/532 with microscope attachment, and (4) KTP/532 laser with 400-μm bare fiber. Each laser was used with the same parameters: 10 watts, 0.4-mm spot size, and continuous- wave mode. Horseradish peroxidase (HRP) was applied to the proximal stump for 30 min, and the animals were sacrificed 24 h later. Horseradish peroxidase (HRP)-labeled motoneuron cell bodies in the lumbar spinal cord were then counted. The average numbers of labeled neurons in each group were as follows: group I (n = 14) 518, group II (n = 8) 424, group III (n = 8) 351, and group IV (n = 8) 283. The standard deviations were quite large, however. When all laser transections were pooled and compared with paired scalpel transections, we found a significant difference, both by the paired t-test (P = 0.016) and by the Wilcoxon matched-paired test (P = 0.02). We conclude that laser transection significantly diminishes the number of neurons labeled by the retrograde transport of HRP.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 43-50 |
| Number of pages | 8 |
| Journal | Lasers in Surgery and Medicine |
| Volume | 12 |
| Issue number | 1 |
| State | Published - 1992 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Surgery
Cite this
Comparative study of laser and scalpel nerve transections. / Fligny, I.; Wu, J. S.; Samonte, B. R.; Fried, Marvin P.
In: Lasers in Surgery and Medicine, Vol. 12, No. 1, 1992, p. 43-50.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Comparative study of laser and scalpel nerve transections
AU - Fligny, I.
AU - Wu, J. S.
AU - Samonte, B. R.
AU - Fried, Marvin P.
PY - 1992
Y1 - 1992
N2 - This investigation was designed to compare standard scalpel transections of the tibial branch of the rat sciatic nerve with those performed using either a milliwatt carbon dioxide (CO2) or a potassium titanyl phosphate (KTP/532) laser. Four transection groups consisted of nerves sectioned with (1) scalpel (control), (2) milliwatt CO2 laser, (3) KTP/532 with microscope attachment, and (4) KTP/532 laser with 400-μm bare fiber. Each laser was used with the same parameters: 10 watts, 0.4-mm spot size, and continuous- wave mode. Horseradish peroxidase (HRP) was applied to the proximal stump for 30 min, and the animals were sacrificed 24 h later. Horseradish peroxidase (HRP)-labeled motoneuron cell bodies in the lumbar spinal cord were then counted. The average numbers of labeled neurons in each group were as follows: group I (n = 14) 518, group II (n = 8) 424, group III (n = 8) 351, and group IV (n = 8) 283. The standard deviations were quite large, however. When all laser transections were pooled and compared with paired scalpel transections, we found a significant difference, both by the paired t-test (P = 0.016) and by the Wilcoxon matched-paired test (P = 0.02). We conclude that laser transection significantly diminishes the number of neurons labeled by the retrograde transport of HRP.
AB - This investigation was designed to compare standard scalpel transections of the tibial branch of the rat sciatic nerve with those performed using either a milliwatt carbon dioxide (CO2) or a potassium titanyl phosphate (KTP/532) laser. Four transection groups consisted of nerves sectioned with (1) scalpel (control), (2) milliwatt CO2 laser, (3) KTP/532 with microscope attachment, and (4) KTP/532 laser with 400-μm bare fiber. Each laser was used with the same parameters: 10 watts, 0.4-mm spot size, and continuous- wave mode. Horseradish peroxidase (HRP) was applied to the proximal stump for 30 min, and the animals were sacrificed 24 h later. Horseradish peroxidase (HRP)-labeled motoneuron cell bodies in the lumbar spinal cord were then counted. The average numbers of labeled neurons in each group were as follows: group I (n = 14) 518, group II (n = 8) 424, group III (n = 8) 351, and group IV (n = 8) 283. The standard deviations were quite large, however. When all laser transections were pooled and compared with paired scalpel transections, we found a significant difference, both by the paired t-test (P = 0.016) and by the Wilcoxon matched-paired test (P = 0.02). We conclude that laser transection significantly diminishes the number of neurons labeled by the retrograde transport of HRP.
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UR - http://www.scopus.com/inward/citedby.url?scp=0026582473&partnerID=8YFLogxK
M3 - Article
VL - 12
SP - 43
EP - 50
JO - Lasers in Surgery and Medicine
JF - Lasers in Surgery and Medicine
SN - 0196-8092
IS - 1
ER -