TY - JOUR
T1 - Comparative requirements for the restriction of retrovirus infection by TRIM5α and TRIMCyp
AU - Diaz-Griffero, Felipe
AU - Kar, Alak
AU - Lee, Mark
AU - Stremlau, Matthew
AU - Poeschla, Eric
AU - Sodroski, Joseph
N1 - Funding Information:
We thank Ms. Yvette McLaughlin and Ms. Elizabeth Carpelan for manuscript preparation, and the National Institutes of Health (AI063987 and a Center for AIDS Research Award AI60354), the International AIDS Vaccine Initiative, the Bristol-Myers Squibb Foundation, and the late William F. McCarty-Cooper. M.S. was supported by a National Defense Science and Engineering Fellowship and was a fellow of the Ryan Foundation.
PY - 2007/12/20
Y1 - 2007/12/20
N2 - The restriction factors, TRIM5α in most primates and TRIMCyp in owl monkeys, block infection of various retroviruses soon after virus entry into the host cell. Rhesus monkey TRIM5α (TRIM5αrh) inhibits human immunodeficiency virus (HIV-1) and feline immunodeficiency virus (FIV) more potently than human TRIM5α (TRIM5αhu). TRIMCyp restricts infection of HIV-1, simian immunodeficiency virus of African green monkeys (SIVagm) and FIV. Early after infection, TRIMCyp, like TRIM5αrh and TRIM5αhu, decreased the amount of particulate viral capsid in the cytosol of infected cells. The requirements for the TRIMCyp and TRIM5α domains in restricting different retroviruses were investigated. Potent restriction of FIV by TRIMCyp occurred in the complete absence of RING and B-box 2 domains; by contrast, efficient FIV restriction by TRIM5αrh required these domains. Variable region 1 of the TRIM5αrh B30.2 domain contributed to the potency of HIV-1, FIV and equine infectious anemia virus restriction. Thus, although differences exist in the requirements of TRIMCyp and TRIM5α for RING/B-box 2 domains, both restriction factors exhibit mechanistic similarities.
AB - The restriction factors, TRIM5α in most primates and TRIMCyp in owl monkeys, block infection of various retroviruses soon after virus entry into the host cell. Rhesus monkey TRIM5α (TRIM5αrh) inhibits human immunodeficiency virus (HIV-1) and feline immunodeficiency virus (FIV) more potently than human TRIM5α (TRIM5αhu). TRIMCyp restricts infection of HIV-1, simian immunodeficiency virus of African green monkeys (SIVagm) and FIV. Early after infection, TRIMCyp, like TRIM5αrh and TRIM5αhu, decreased the amount of particulate viral capsid in the cytosol of infected cells. The requirements for the TRIMCyp and TRIM5α domains in restricting different retroviruses were investigated. Potent restriction of FIV by TRIMCyp occurred in the complete absence of RING and B-box 2 domains; by contrast, efficient FIV restriction by TRIM5αrh required these domains. Variable region 1 of the TRIM5αrh B30.2 domain contributed to the potency of HIV-1, FIV and equine infectious anemia virus restriction. Thus, although differences exist in the requirements of TRIMCyp and TRIM5α for RING/B-box 2 domains, both restriction factors exhibit mechanistic similarities.
KW - Human immunodeficiency virus
KW - Restriction factors
KW - Retrovirus
KW - Uncoating
UR - http://www.scopus.com/inward/record.url?scp=36148994747&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=36148994747&partnerID=8YFLogxK
U2 - 10.1016/j.virol.2007.08.032
DO - 10.1016/j.virol.2007.08.032
M3 - Article
C2 - 17920096
AN - SCOPUS:36148994747
SN - 0042-6822
VL - 369
SP - 400
EP - 410
JO - Virology
JF - Virology
IS - 2
ER -
