Comparative proteomic analysis of histone post-translational modifications upon ischemia/reperfusion-induced retinal injury

Xiaolu Zhao, Simone Sidoli, Leilei Wang, Wenjun Wang, Lin Guo, Ole N. Jensen, Ling Zheng

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

We present a detailed quantitative map of single and coexisting histone post-translational modifications (PTMs) in rat retinas affected by ischemia and reperfusion (I/R) injury. Retinal I/R injury contributes to serious ocular diseases, which can lead to vision loss and blindness. We applied linear ion trap-orbitrap hybrid tandem mass spectrometry (MS/MS) to quantify 131 single histone marks and 143 combinations of multiple histone marks in noninjured and injured retinas. We observed 34 histone PTMs that exhibited significantly (p < 0.05) different abundance between healthy and I/R injured eyes, of which we confirmed three H4 histone marks by Western blotting. H4K20me2 was up to 4-fold change up-regulated after the injury and is associated with the response to DNA damage as demonstrated by an increase in the phosphorylation of p53 and Chk1. This study demonstrates that quantitative MS provides a sensitive and accurate way to dissect the changes in the histone code after retinal injury. Specifically, DNA damage associated histone PTMs may contribute to neurovascular degeneration during the process of ischemia/reperfusion injury.

Original languageEnglish (US)
Pages (from-to)2175-2186
Number of pages12
JournalJournal of Proteome Research
Volume13
Issue number4
DOIs
StatePublished - Apr 4 2014
Externally publishedYes

Fingerprint

Histone Code
Post Translational Protein Processing
Proteomics
Histones
Reperfusion
Ischemia
Reperfusion Injury
Wounds and Injuries
DNA Damage
Retina
Eye Diseases
Blindness
Tandem Mass Spectrometry
Western Blotting
Phosphorylation
Ions
DNA
Mass spectrometry
Rats

Keywords

  • DNA damage
  • histones
  • ischemia/reperfusion injury
  • label-free quantitation
  • LC-MS/MS
  • PTMs
  • retina

ASJC Scopus subject areas

  • Biochemistry
  • Chemistry(all)

Cite this

Comparative proteomic analysis of histone post-translational modifications upon ischemia/reperfusion-induced retinal injury. / Zhao, Xiaolu; Sidoli, Simone; Wang, Leilei; Wang, Wenjun; Guo, Lin; Jensen, Ole N.; Zheng, Ling.

In: Journal of Proteome Research, Vol. 13, No. 4, 04.04.2014, p. 2175-2186.

Research output: Contribution to journalArticle

Zhao, Xiaolu ; Sidoli, Simone ; Wang, Leilei ; Wang, Wenjun ; Guo, Lin ; Jensen, Ole N. ; Zheng, Ling. / Comparative proteomic analysis of histone post-translational modifications upon ischemia/reperfusion-induced retinal injury. In: Journal of Proteome Research. 2014 ; Vol. 13, No. 4. pp. 2175-2186.
@article{469bb2d80e7b495fb7d1bcb5148a989a,
title = "Comparative proteomic analysis of histone post-translational modifications upon ischemia/reperfusion-induced retinal injury",
abstract = "We present a detailed quantitative map of single and coexisting histone post-translational modifications (PTMs) in rat retinas affected by ischemia and reperfusion (I/R) injury. Retinal I/R injury contributes to serious ocular diseases, which can lead to vision loss and blindness. We applied linear ion trap-orbitrap hybrid tandem mass spectrometry (MS/MS) to quantify 131 single histone marks and 143 combinations of multiple histone marks in noninjured and injured retinas. We observed 34 histone PTMs that exhibited significantly (p < 0.05) different abundance between healthy and I/R injured eyes, of which we confirmed three H4 histone marks by Western blotting. H4K20me2 was up to 4-fold change up-regulated after the injury and is associated with the response to DNA damage as demonstrated by an increase in the phosphorylation of p53 and Chk1. This study demonstrates that quantitative MS provides a sensitive and accurate way to dissect the changes in the histone code after retinal injury. Specifically, DNA damage associated histone PTMs may contribute to neurovascular degeneration during the process of ischemia/reperfusion injury.",
keywords = "DNA damage, histones, ischemia/reperfusion injury, label-free quantitation, LC-MS/MS, PTMs, retina",
author = "Xiaolu Zhao and Simone Sidoli and Leilei Wang and Wenjun Wang and Lin Guo and Jensen, {Ole N.} and Ling Zheng",
year = "2014",
month = "4",
day = "4",
doi = "10.1021/pr500040a",
language = "English (US)",
volume = "13",
pages = "2175--2186",
journal = "Journal of Proteome Research",
issn = "1535-3893",
publisher = "American Chemical Society",
number = "4",

}

TY - JOUR

T1 - Comparative proteomic analysis of histone post-translational modifications upon ischemia/reperfusion-induced retinal injury

AU - Zhao, Xiaolu

AU - Sidoli, Simone

AU - Wang, Leilei

AU - Wang, Wenjun

AU - Guo, Lin

AU - Jensen, Ole N.

AU - Zheng, Ling

PY - 2014/4/4

Y1 - 2014/4/4

N2 - We present a detailed quantitative map of single and coexisting histone post-translational modifications (PTMs) in rat retinas affected by ischemia and reperfusion (I/R) injury. Retinal I/R injury contributes to serious ocular diseases, which can lead to vision loss and blindness. We applied linear ion trap-orbitrap hybrid tandem mass spectrometry (MS/MS) to quantify 131 single histone marks and 143 combinations of multiple histone marks in noninjured and injured retinas. We observed 34 histone PTMs that exhibited significantly (p < 0.05) different abundance between healthy and I/R injured eyes, of which we confirmed three H4 histone marks by Western blotting. H4K20me2 was up to 4-fold change up-regulated after the injury and is associated with the response to DNA damage as demonstrated by an increase in the phosphorylation of p53 and Chk1. This study demonstrates that quantitative MS provides a sensitive and accurate way to dissect the changes in the histone code after retinal injury. Specifically, DNA damage associated histone PTMs may contribute to neurovascular degeneration during the process of ischemia/reperfusion injury.

AB - We present a detailed quantitative map of single and coexisting histone post-translational modifications (PTMs) in rat retinas affected by ischemia and reperfusion (I/R) injury. Retinal I/R injury contributes to serious ocular diseases, which can lead to vision loss and blindness. We applied linear ion trap-orbitrap hybrid tandem mass spectrometry (MS/MS) to quantify 131 single histone marks and 143 combinations of multiple histone marks in noninjured and injured retinas. We observed 34 histone PTMs that exhibited significantly (p < 0.05) different abundance between healthy and I/R injured eyes, of which we confirmed three H4 histone marks by Western blotting. H4K20me2 was up to 4-fold change up-regulated after the injury and is associated with the response to DNA damage as demonstrated by an increase in the phosphorylation of p53 and Chk1. This study demonstrates that quantitative MS provides a sensitive and accurate way to dissect the changes in the histone code after retinal injury. Specifically, DNA damage associated histone PTMs may contribute to neurovascular degeneration during the process of ischemia/reperfusion injury.

KW - DNA damage

KW - histones

KW - ischemia/reperfusion injury

KW - label-free quantitation

KW - LC-MS/MS

KW - PTMs

KW - retina

UR - http://www.scopus.com/inward/record.url?scp=84898761490&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84898761490&partnerID=8YFLogxK

U2 - 10.1021/pr500040a

DO - 10.1021/pr500040a

M3 - Article

C2 - 24628298

AN - SCOPUS:84898761490

VL - 13

SP - 2175

EP - 2186

JO - Journal of Proteome Research

JF - Journal of Proteome Research

SN - 1535-3893

IS - 4

ER -