Comparative analysis of polytene chromosome banding pattern of closely related species clyptotendipes salinus michailova and G. barbipes staeger (Diptera, Chironomidae)

Karyotype structure and chromosomal polymorphism of Glyptotendipes salinus from Altai and Kazachstan populations were studied. A cytophotomap of G. salinus salivary gland polytene chromosomes is presented. Comparative analysis of G. salinus and G. barbipes polytene chromosomes banding patterns has shown that G. salinus was less polymorphic on set of inversion sequences than G. barbipes (11 and 47 inversion sequences correspondently). Chromosome arms C and G are polymorphic in G. salinus only, where as all 7 polymorphic chromosome arms are polymorphic in G. barbipes. The level of chromosome polymorphism was lower in G. salinus than that in G. barbipes: from 48 to 62 % larvae in populations of this species are heterozygotes on inversions in comparison with 72-100 % inversion heterozygotes in G. barbipes. The high frequency of centromeric heterochromatin heterozygosity in arm G (from 6 to 30 % in different populations). Cytogenetic distances between G. salinus populations studied were determined. A very low level of population differentiation (Dcg = 0.015 ± 0.013) was demonstrated. Cytogenetic distances between G. salinus and G. barbipes population were also determined. It corresponds to the difference between morphological species (Dcg= 1.435 ± 0.019).