Comparative analysis of meiotic progression in female mice bearing mutations in genes of the DNA mismatch repair pathway

Rui Kan, Xianfei Sun, Nadine K. Kolas, Elena Avdievich, Burkhard Kneitz, Winfried Edelmann, Paula E. Cohen

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

The DNA mismatch repair (MMR) family functions in a variety of contexts to preserve genome integrity in most eukaryotes. In particular, members of the MMR family are involved in the process of meiotic recombination in germ cells. MMR gene mutations in mice result in meiotic disruption during prophase I, but the extent of this disruption often differs between male and female meiocytes. To address the role of MMR proteins specifically in female meiosis, we explored the progression of oocytes through prophase I and the meiotic divisions in mice harboring deletions in members of the MMR pathway (Mlh1, Mlh3, Exo1, and an ATPase-deficient variant of Mlh1, Mlh1G67R). The colocalization of MLH1 and MLH3, key proteins involved in stabilization of nascent crossovers, was dependent on intact heterodimer formation and was highly correlated with the ability of oocytes to progress through to metaphase II. The exception was Exo1-/- oocytes, in which normal MLH1/MLH3 localization was observed followed by failure to proceed to metaphase II. All mutant oocytes were able to resume meiosis after dictyate arrest, but they showed a dramatic decline in chiasmata (to less than 25% of normal), accompanied by varied progression through metaphase I. Taken together, these results demonstrate that MMR function is required for the formation and stabilization of crossovers in mammalian oocytes and that, in the absence of a functional MMR system, the failure to maintain chiasmata results in a reduced ability to proceed normally through the first and second meiotic divisions, despite near-normal levels of meiotic resumption after dictyate arrest.

Original languageEnglish (US)
Pages (from-to)462-471
Number of pages10
JournalBiology of Reproduction
Volume78
Issue number3
DOIs
StatePublished - Mar 2008

Fingerprint

DNA Mismatch Repair
Oocytes
Mutation
Genes
Metaphase
Meiotic Prophase I
Meiosis
Eukaryota
Germ Cells
Genetic Recombination
Adenosine Triphosphatases
Proteins
Genome

Keywords

  • Gamete biology
  • Gametogenesis
  • Meiosis
  • Mismatch repair
  • Oocyte development
  • Ovary
  • Recombination
  • Synaptonemal complex

ASJC Scopus subject areas

  • Cell Biology
  • Developmental Biology
  • Embryology

Cite this

Comparative analysis of meiotic progression in female mice bearing mutations in genes of the DNA mismatch repair pathway. / Kan, Rui; Sun, Xianfei; Kolas, Nadine K.; Avdievich, Elena; Kneitz, Burkhard; Edelmann, Winfried; Cohen, Paula E.

In: Biology of Reproduction, Vol. 78, No. 3, 03.2008, p. 462-471.

Research output: Contribution to journalArticle

Kan, Rui ; Sun, Xianfei ; Kolas, Nadine K. ; Avdievich, Elena ; Kneitz, Burkhard ; Edelmann, Winfried ; Cohen, Paula E. / Comparative analysis of meiotic progression in female mice bearing mutations in genes of the DNA mismatch repair pathway. In: Biology of Reproduction. 2008 ; Vol. 78, No. 3. pp. 462-471.
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