TY - JOUR
T1 - Combined use of serum and urinary antibody for diagnosis of tuberculosis
AU - Singh, Krishna K.
AU - Dong, Yuxin
AU - Hinds, Laura
AU - Keen, Marc A.
AU - Belisle, John T.
AU - Zolla-Pazner, Susan
AU - Achkar, Jacqueline M.
AU - Nadas, Arthur J.
AU - Arora, Vijay K.
AU - Laal, Suman
N1 - Funding Information:
Antigens. The lipoarabinomannan (LAM)–free CFP of M. tuberculosis H37Rv have been used in all our previous studies of humoral responses to M. tuberculosis [3–5, 23] and is available from the National Institutes of Health/National Institute of Allergy and Infectious Diseases Tuberculosis Research Materials and Vaccine Testing, Colorado State University (Fort Collins). This preparation has been used recently by us to delineate the profiles of serodominant antigens recognized by antibodies from patients with TB at different stages of disease progression [5, 24].
Funding Information:
Financial support: Research Center for AIDS and HIV infection, New York Harbor Health Care System; National Institutes of Health (grant AI-36984); National Institute for Allergy and Infectious Diseases (contract NO1-AI-75320, entitled “Tuberculosis Research Materials and Vaccine Testing”).
PY - 2003/8/1
Y1 - 2003/8/1
N2 - Efforts to devise immunoassays for tuberculosis (TB) that can be adapted to rapid formats are ongoing. The present study was aimed at determining whether urinary anti-Mycobacterium tuberculosis antibodies are present in patients with TB, to evaluate the feasibility of developing a urine antibody-based diagnostic test. Urinary antibodies directed against the culture filtrate proteins of M. tuberculosis, MPT 32, and the 81-kDa GlcB protein were detectable in patients with TB, although the sensitivity of antibody detection was lower (53%-64%), compared with serum antibodies (68%-77%). Surprisingly, with all 3 antigens, the use of paired serum and urine samples provided higher sensitivities of antibody detection than either single specimen, and anti-GlcB antibodies were present in the serum and/or urine of 39 (90%) of 43 smear-positive patients with TB. Although, with the current methods and antigens, the level of sensitivity is insufficient to design a urinary antibody diagnostic test, these studies provide the foundation for further studies on the development of a urine antibody-based immunoassay for TB.
AB - Efforts to devise immunoassays for tuberculosis (TB) that can be adapted to rapid formats are ongoing. The present study was aimed at determining whether urinary anti-Mycobacterium tuberculosis antibodies are present in patients with TB, to evaluate the feasibility of developing a urine antibody-based diagnostic test. Urinary antibodies directed against the culture filtrate proteins of M. tuberculosis, MPT 32, and the 81-kDa GlcB protein were detectable in patients with TB, although the sensitivity of antibody detection was lower (53%-64%), compared with serum antibodies (68%-77%). Surprisingly, with all 3 antigens, the use of paired serum and urine samples provided higher sensitivities of antibody detection than either single specimen, and anti-GlcB antibodies were present in the serum and/or urine of 39 (90%) of 43 smear-positive patients with TB. Although, with the current methods and antigens, the level of sensitivity is insufficient to design a urinary antibody diagnostic test, these studies provide the foundation for further studies on the development of a urine antibody-based immunoassay for TB.
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U2 - 10.1086/376532
DO - 10.1086/376532
M3 - Article
C2 - 12870118
AN - SCOPUS:0042658352
SN - 0022-1899
VL - 188
SP - 371
EP - 377
JO - Journal of Infectious Diseases
JF - Journal of Infectious Diseases
IS - 3
ER -
