Combined use of serum and urinary antibody for diagnosis of tuberculosis

Krishna K. Singh, Yuxin Dong, Laura Hinds, Marc A. Keen, John T. Belisle, Susan Zolla-Pazner, Jacqueline M. Achkar, Arthur J. Nadas, Vijay K. Arora, Suman Laal

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25 Scopus citations

Abstract

Efforts to devise immunoassays for tuberculosis (TB) that can be adapted to rapid formats are ongoing. The present study was aimed at determining whether urinary anti-Mycobacterium tuberculosis antibodies are present in patients with TB, to evaluate the feasibility of developing a urine antibody-based diagnostic test. Urinary antibodies directed against the culture filtrate proteins of M. tuberculosis, MPT 32, and the 81-kDa GlcB protein were detectable in patients with TB, although the sensitivity of antibody detection was lower (53%-64%), compared with serum antibodies (68%-77%). Surprisingly, with all 3 antigens, the use of paired serum and urine samples provided higher sensitivities of antibody detection than either single specimen, and anti-GlcB antibodies were present in the serum and/or urine of 39 (90%) of 43 smear-positive patients with TB. Although, with the current methods and antigens, the level of sensitivity is insufficient to design a urinary antibody diagnostic test, these studies provide the foundation for further studies on the development of a urine antibody-based immunoassay for TB.

Original languageEnglish (US)
Pages (from-to)371-377
Number of pages7
JournalJournal of Infectious Diseases
Volume188
Issue number3
DOIs
Publication statusPublished - Aug 1 2003
Externally publishedYes

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ASJC Scopus subject areas

  • Immunology and Allergy
  • Infectious Diseases

Cite this

Singh, K. K., Dong, Y., Hinds, L., Keen, M. A., Belisle, J. T., Zolla-Pazner, S., ... Laal, S. (2003). Combined use of serum and urinary antibody for diagnosis of tuberculosis. Journal of Infectious Diseases, 188(3), 371-377. https://doi.org/10.1086/376532