Combined use of serum and urinary antibody for diagnosis of tuberculosis

Krishna K. Singh, Yuxin Dong, Laura Hinds, Marc A. Keen, John T. Belisle, Susan Zolla-Pazner, Jacqueline M. Achkar, Arthur J. Nadas, Vijay K. Arora, Suman Laal

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

Efforts to devise immunoassays for tuberculosis (TB) that can be adapted to rapid formats are ongoing. The present study was aimed at determining whether urinary anti-Mycobacterium tuberculosis antibodies are present in patients with TB, to evaluate the feasibility of developing a urine antibody-based diagnostic test. Urinary antibodies directed against the culture filtrate proteins of M. tuberculosis, MPT 32, and the 81-kDa GlcB protein were detectable in patients with TB, although the sensitivity of antibody detection was lower (53%-64%), compared with serum antibodies (68%-77%). Surprisingly, with all 3 antigens, the use of paired serum and urine samples provided higher sensitivities of antibody detection than either single specimen, and anti-GlcB antibodies were present in the serum and/or urine of 39 (90%) of 43 smear-positive patients with TB. Although, with the current methods and antigens, the level of sensitivity is insufficient to design a urinary antibody diagnostic test, these studies provide the foundation for further studies on the development of a urine antibody-based immunoassay for TB.

Original languageEnglish (US)
Pages (from-to)371-377
Number of pages7
JournalJournal of Infectious Diseases
Volume188
Issue number3
DOIs
StatePublished - Aug 1 2003
Externally publishedYes

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Tuberculosis
Antibodies
Serum
Urine
Mycobacterium tuberculosis
Immunoassay
Routine Diagnostic Tests
Antigens
Anti-Idiotypic Antibodies
Proteins

ASJC Scopus subject areas

  • Public Health, Environmental and Occupational Health
  • Immunology

Cite this

Singh, K. K., Dong, Y., Hinds, L., Keen, M. A., Belisle, J. T., Zolla-Pazner, S., ... Laal, S. (2003). Combined use of serum and urinary antibody for diagnosis of tuberculosis. Journal of Infectious Diseases, 188(3), 371-377. https://doi.org/10.1086/376532

Combined use of serum and urinary antibody for diagnosis of tuberculosis. / Singh, Krishna K.; Dong, Yuxin; Hinds, Laura; Keen, Marc A.; Belisle, John T.; Zolla-Pazner, Susan; Achkar, Jacqueline M.; Nadas, Arthur J.; Arora, Vijay K.; Laal, Suman.

In: Journal of Infectious Diseases, Vol. 188, No. 3, 01.08.2003, p. 371-377.

Research output: Contribution to journalArticle

Singh, KK, Dong, Y, Hinds, L, Keen, MA, Belisle, JT, Zolla-Pazner, S, Achkar, JM, Nadas, AJ, Arora, VK & Laal, S 2003, 'Combined use of serum and urinary antibody for diagnosis of tuberculosis', Journal of Infectious Diseases, vol. 188, no. 3, pp. 371-377. https://doi.org/10.1086/376532
Singh KK, Dong Y, Hinds L, Keen MA, Belisle JT, Zolla-Pazner S et al. Combined use of serum and urinary antibody for diagnosis of tuberculosis. Journal of Infectious Diseases. 2003 Aug 1;188(3):371-377. https://doi.org/10.1086/376532
Singh, Krishna K. ; Dong, Yuxin ; Hinds, Laura ; Keen, Marc A. ; Belisle, John T. ; Zolla-Pazner, Susan ; Achkar, Jacqueline M. ; Nadas, Arthur J. ; Arora, Vijay K. ; Laal, Suman. / Combined use of serum and urinary antibody for diagnosis of tuberculosis. In: Journal of Infectious Diseases. 2003 ; Vol. 188, No. 3. pp. 371-377.
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