Colony stimulating factor-1 (CSF-1) stimulates temperature dependent phosphorylation and activation of the RAF-1 proto-oncogene product

Manuela Baccarini, David M. Sabatini, Harald App, Ulf R. Rapp, E. Richard Stanley

Research output: Contribution to journalArticle

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Abstract

The serine/threonine kinase RAF-1 is phosphorylated in intact macrophages in response to CSF-1 at 37°C The augmented phosphorylation of RAF-1 and a concomitant increase in RAF-1 associated serine/threonine kinase activity are kinetically later events than CSF-1 induced protein tyrosine phosphorylation. Furthermore, phosphoamino acid analysis of RAF-1 reveals the presence of phosphoserine, trace amounts of phosphothreonine but no phosphotyrosine and the phosphorylated RAF-1 does not react with anti-phosphotyrosine antibodies. In contrast to CSF-1 induced protein tyrosine phosphorylation, RAF-1 phosphorylation and activation are temperature dependent and do not occur at 4°C. Furthermore, coprecipitation experiments failed to reveal any non-covalent association of RAF-1 with the CSF-1 receptor. Thus, while RAF-1 is not a direct substrate for the CSF-1 receptor tyrosine kinase in vivo, its temperature dependent phosphorylation and activation represent an intriguing aspect of the CSF-1 response.

Original languageEnglish (US)
Pages (from-to)3649-3657
Number of pages9
JournalEMBO Journal
Volume9
Issue number11
StatePublished - 1990

Fingerprint

Phosphorylation
Macrophage Colony-Stimulating Factor
Proto-Oncogenes
Oncogene Proteins
Chemical activation
Temperature
Colony-Stimulating Factor Receptors
Phosphotyrosine
Protein-Serine-Threonine Kinases
Tyrosine
Phosphothreonine
Phosphoamino Acids
Phosphoserine
Macrophages
Receptor Protein-Tyrosine Kinases
Coprecipitation
Protein-Tyrosine Kinases
Anti-Idiotypic Antibodies
Proteins
Association reactions

Keywords

  • CSF-1
  • Growth factor receptors
  • PDGF
  • RAF-1 kinase
  • Signal transduction

ASJC Scopus subject areas

  • Genetics
  • Cell Biology

Cite this

Baccarini, M., Sabatini, D. M., App, H., Rapp, U. R., & Stanley, E. R. (1990). Colony stimulating factor-1 (CSF-1) stimulates temperature dependent phosphorylation and activation of the RAF-1 proto-oncogene product. EMBO Journal, 9(11), 3649-3657.

Colony stimulating factor-1 (CSF-1) stimulates temperature dependent phosphorylation and activation of the RAF-1 proto-oncogene product. / Baccarini, Manuela; Sabatini, David M.; App, Harald; Rapp, Ulf R.; Stanley, E. Richard.

In: EMBO Journal, Vol. 9, No. 11, 1990, p. 3649-3657.

Research output: Contribution to journalArticle

Baccarini, M, Sabatini, DM, App, H, Rapp, UR & Stanley, ER 1990, 'Colony stimulating factor-1 (CSF-1) stimulates temperature dependent phosphorylation and activation of the RAF-1 proto-oncogene product', EMBO Journal, vol. 9, no. 11, pp. 3649-3657.
Baccarini, Manuela ; Sabatini, David M. ; App, Harald ; Rapp, Ulf R. ; Stanley, E. Richard. / Colony stimulating factor-1 (CSF-1) stimulates temperature dependent phosphorylation and activation of the RAF-1 proto-oncogene product. In: EMBO Journal. 1990 ; Vol. 9, No. 11. pp. 3649-3657.
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