Colocalization of H+-ATPase and band 3 anion exchanger in rabbit collecting duct intercalated cells

Victor L. Schuster, Geza Fejes-Toth, Aniko Naray-Fejes-Toth, Stephen Gluck

Research output: Contribution to journalArticle

81 Citations (Scopus)

Abstract

Two major types of interca lated cells (IC) have been previously defined in rabbit collecting duct: α-cells have a basolateral band 3-like anion exchanger and secrete H+, whereas β-cells bind peanut agglutinin (PNA) apically and are believed to secrete HCO3-. To further define IC types, we double-labeled kidney sections with anti-H+-ATPase antibodies and with either an anti-band 3 antibody or PNA. We found four patterns of staining: 1) IC with apical H+-ATPase and basal band 3, a configuration consistent with ongoing H+ secretion, which prevailed in the inner stripe of outer medulla (OMCDi); 2) diffuse H+-ATPase labeling across the cell and basal band 3, which was most numerous in the outer stripe of outer medulla (OMCDo); 3) IC with "bright" apical peanut lectin, diffuse H+-ATPase, and no band 3, which was abundant in the cortical collecting duct (CCD) and probably represents HCO3--secreting cells; and 4) "hybrid" cells with various staining combinations (e.g., apical lectin binding and apical H+-ATPase), which although they are uncommon, were seen in the CCD. Consistent with this immunocytochemical finding of hybrid cells, cell-sorting studies on isolated CCD IC showed that 6-18% of PNA-positive cells also stained positively for band 3. We conclude that 1) band 3-positive IC in the OMCD vary axially. Most OMCDi IC are probably active proton secretors, whereas up to one-half of OMCDo IC may be latent H+ secretors. 2) The diffuse H+-ATPase pattern in putative β-cells differs from comparable results in the rat and is not consistent with a "reversed" α-cell. HCO3- secretion by β-cells may be driven by an H+ extrusion mechanism other than the α-cell pump re-sorted to the basolateral membrane. 3) The possibility of hybrid cells that might combine α- and α-cell transport proteins suggests a mechanism for functional reversal of collecting duct IC polarity.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Renal Fluid and Electrolyte Physiology
Volume260
Issue number4 29-4
StatePublished - 1991
Externally publishedYes

Fingerprint

Chloride-Bicarbonate Antiporters
Proton-Translocating ATPases
Rabbits
Peanut Agglutinin
Hybrid Cells

Keywords

  • Acid
  • Acidification
  • Alkalinization
  • Alkalosis
  • Antirfort
  • Bicarbonate
  • Chloride
  • Epithelial polarity
  • Fluorescence-activated cell sorting
  • Immunocytochemistry
  • Lectins
  • Mitochondria

ASJC Scopus subject areas

  • Physiology

Cite this

Colocalization of H+-ATPase and band 3 anion exchanger in rabbit collecting duct intercalated cells. / Schuster, Victor L.; Fejes-Toth, Geza; Naray-Fejes-Toth, Aniko; Gluck, Stephen.

In: American Journal of Physiology - Renal Fluid and Electrolyte Physiology, Vol. 260, No. 4 29-4, 1991.

Research output: Contribution to journalArticle

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abstract = "Two major types of interca lated cells (IC) have been previously defined in rabbit collecting duct: α-cells have a basolateral band 3-like anion exchanger and secrete H+, whereas β-cells bind peanut agglutinin (PNA) apically and are believed to secrete HCO3-. To further define IC types, we double-labeled kidney sections with anti-H+-ATPase antibodies and with either an anti-band 3 antibody or PNA. We found four patterns of staining: 1) IC with apical H+-ATPase and basal band 3, a configuration consistent with ongoing H+ secretion, which prevailed in the inner stripe of outer medulla (OMCDi); 2) diffuse H+-ATPase labeling across the cell and basal band 3, which was most numerous in the outer stripe of outer medulla (OMCDo); 3) IC with {"}bright{"} apical peanut lectin, diffuse H+-ATPase, and no band 3, which was abundant in the cortical collecting duct (CCD) and probably represents HCO3--secreting cells; and 4) {"}hybrid{"} cells with various staining combinations (e.g., apical lectin binding and apical H+-ATPase), which although they are uncommon, were seen in the CCD. Consistent with this immunocytochemical finding of hybrid cells, cell-sorting studies on isolated CCD IC showed that 6-18{\%} of PNA-positive cells also stained positively for band 3. We conclude that 1) band 3-positive IC in the OMCD vary axially. Most OMCDi IC are probably active proton secretors, whereas up to one-half of OMCDo IC may be latent H+ secretors. 2) The diffuse H+-ATPase pattern in putative β-cells differs from comparable results in the rat and is not consistent with a {"}reversed{"} α-cell. HCO3- secretion by β-cells may be driven by an H+ extrusion mechanism other than the α-cell pump re-sorted to the basolateral membrane. 3) The possibility of hybrid cells that might combine α- and α-cell transport proteins suggests a mechanism for functional reversal of collecting duct IC polarity.",
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T1 - Colocalization of H+-ATPase and band 3 anion exchanger in rabbit collecting duct intercalated cells

AU - Schuster, Victor L.

AU - Fejes-Toth, Geza

AU - Naray-Fejes-Toth, Aniko

AU - Gluck, Stephen

PY - 1991

Y1 - 1991

N2 - Two major types of interca lated cells (IC) have been previously defined in rabbit collecting duct: α-cells have a basolateral band 3-like anion exchanger and secrete H+, whereas β-cells bind peanut agglutinin (PNA) apically and are believed to secrete HCO3-. To further define IC types, we double-labeled kidney sections with anti-H+-ATPase antibodies and with either an anti-band 3 antibody or PNA. We found four patterns of staining: 1) IC with apical H+-ATPase and basal band 3, a configuration consistent with ongoing H+ secretion, which prevailed in the inner stripe of outer medulla (OMCDi); 2) diffuse H+-ATPase labeling across the cell and basal band 3, which was most numerous in the outer stripe of outer medulla (OMCDo); 3) IC with "bright" apical peanut lectin, diffuse H+-ATPase, and no band 3, which was abundant in the cortical collecting duct (CCD) and probably represents HCO3--secreting cells; and 4) "hybrid" cells with various staining combinations (e.g., apical lectin binding and apical H+-ATPase), which although they are uncommon, were seen in the CCD. Consistent with this immunocytochemical finding of hybrid cells, cell-sorting studies on isolated CCD IC showed that 6-18% of PNA-positive cells also stained positively for band 3. We conclude that 1) band 3-positive IC in the OMCD vary axially. Most OMCDi IC are probably active proton secretors, whereas up to one-half of OMCDo IC may be latent H+ secretors. 2) The diffuse H+-ATPase pattern in putative β-cells differs from comparable results in the rat and is not consistent with a "reversed" α-cell. HCO3- secretion by β-cells may be driven by an H+ extrusion mechanism other than the α-cell pump re-sorted to the basolateral membrane. 3) The possibility of hybrid cells that might combine α- and α-cell transport proteins suggests a mechanism for functional reversal of collecting duct IC polarity.

AB - Two major types of interca lated cells (IC) have been previously defined in rabbit collecting duct: α-cells have a basolateral band 3-like anion exchanger and secrete H+, whereas β-cells bind peanut agglutinin (PNA) apically and are believed to secrete HCO3-. To further define IC types, we double-labeled kidney sections with anti-H+-ATPase antibodies and with either an anti-band 3 antibody or PNA. We found four patterns of staining: 1) IC with apical H+-ATPase and basal band 3, a configuration consistent with ongoing H+ secretion, which prevailed in the inner stripe of outer medulla (OMCDi); 2) diffuse H+-ATPase labeling across the cell and basal band 3, which was most numerous in the outer stripe of outer medulla (OMCDo); 3) IC with "bright" apical peanut lectin, diffuse H+-ATPase, and no band 3, which was abundant in the cortical collecting duct (CCD) and probably represents HCO3--secreting cells; and 4) "hybrid" cells with various staining combinations (e.g., apical lectin binding and apical H+-ATPase), which although they are uncommon, were seen in the CCD. Consistent with this immunocytochemical finding of hybrid cells, cell-sorting studies on isolated CCD IC showed that 6-18% of PNA-positive cells also stained positively for band 3. We conclude that 1) band 3-positive IC in the OMCD vary axially. Most OMCDi IC are probably active proton secretors, whereas up to one-half of OMCDo IC may be latent H+ secretors. 2) The diffuse H+-ATPase pattern in putative β-cells differs from comparable results in the rat and is not consistent with a "reversed" α-cell. HCO3- secretion by β-cells may be driven by an H+ extrusion mechanism other than the α-cell pump re-sorted to the basolateral membrane. 3) The possibility of hybrid cells that might combine α- and α-cell transport proteins suggests a mechanism for functional reversal of collecting duct IC polarity.

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KW - Bicarbonate

KW - Chloride

KW - Epithelial polarity

KW - Fluorescence-activated cell sorting

KW - Immunocytochemistry

KW - Lectins

KW - Mitochondria

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SN - 1931-857X

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