Collisional activation decomposition of actinomycin using tandem mass spectrometry

J. Roboz, Edward Nieves, J. F. Holland, M. McCamish, C. Smith

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

The collisional activation (xenon) decomposition of actinomycin V, dactinomycin, 7-nitrodactinomycin, 7-aminodactinomycin, and a mixture of dactinomycin, actinomycin C1 and actinomycin C2 was studied using a triple-quadrupole mass spectrometer with fast and atom bombardment (xenon) ionization. Fragmentation pathways of the structurally significant ions were rationalized by assuming an initial McLafferty rearrangement at the ester linkage between the methylvaline and the threonine of both rings to form linear pentapeptides, followed by fragmentation in either pentapeptide yielding typical sequence ions and sequence cleavages from both rings.

Original languageEnglish (US)
Pages (from-to)67-70
Number of pages4
JournalBiomedical and Environmental Mass Spectrometry
Volume16
Issue number1-12 SPEC. ISS.
StatePublished - 1988
Externally publishedYes

Fingerprint

Xenon
Dactinomycin
Tandem Mass Spectrometry
Mass spectrometry
Chemical activation
Decomposition
Ions
Mass spectrometers
Ionization
Esters
Atoms
Threonine

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Medicine
  • Pollution

Cite this

Collisional activation decomposition of actinomycin using tandem mass spectrometry. / Roboz, J.; Nieves, Edward; Holland, J. F.; McCamish, M.; Smith, C.

In: Biomedical and Environmental Mass Spectrometry, Vol. 16, No. 1-12 SPEC. ISS., 1988, p. 67-70.

Research output: Contribution to journalArticle

Roboz, J, Nieves, E, Holland, JF, McCamish, M & Smith, C 1988, 'Collisional activation decomposition of actinomycin using tandem mass spectrometry', Biomedical and Environmental Mass Spectrometry, vol. 16, no. 1-12 SPEC. ISS., pp. 67-70.
Roboz, J. ; Nieves, Edward ; Holland, J. F. ; McCamish, M. ; Smith, C. / Collisional activation decomposition of actinomycin using tandem mass spectrometry. In: Biomedical and Environmental Mass Spectrometry. 1988 ; Vol. 16, No. 1-12 SPEC. ISS. pp. 67-70.
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