Collagenase degrades collagen in vivo in the ischemic heart

S. Takahashi, D. Geenen, E. Nieves, T. Iwazumi

Research output: Contribution to journalArticle

7 Scopus citations

Abstract

Previously, we showed that ischemic rat heart contains an activated procollagenase capable of degrading collagen in vitro. We now demonstrate that the collagen resident in such hearts (in vivo) also becomes degraded, producing characteristic fragments implicating the action of an activated collagenase. The evidence is the appearance of amino-terminal dansyl-Ile (+dansyl-Leu) residues in pepsin digests of re-oxygenated rat hearts and immunoblots showing 3/4 length (αA) fragments from type I collagen. Also, in ischemic rat myocardium, αA(I) and αA(III) fragments were detected in pepsin digests. The time periods required for the cleavage and degradation of collagen suggest the participation of a procollagenase that becomes activated. Results demonstrate for the first time that an interstitial collagenase in such hearts initiates in vivo degradation of types I and III collagens. Copyright (C) 1999 Elsevier Science B.V.

Original languageEnglish (US)
Pages (from-to)251-259
Number of pages9
JournalBiochimica et Biophysica Acta - General Subjects
Volume1428
Issue number2-3
DOIs
StatePublished - Aug 5 1999

Keywords

  • Collagen
  • Collagenase
  • Ischemic myocardium

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology

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