Cofilin Promotes Actin Polymerization and Defines the Direction of Cell Motility

Mousumi Ghosh; Xiaoyan Song; Ghassan Mouneimne; Mazen Sidani; David S. Lawrence; John S. Condeelis

doi:10.1126/science.1094561

Cofilin Promotes Actin Polymerization and Defines the Direction of Cell Motility

Mousumi Ghosh, Xiaoyan Song, Ghassan Mouneimne, Mazen Sidani, David S. Lawrence, John S. Condeelis

Cell Biology

Research output: Contribution to journal › Article › peer-review

567 Scopus citations

Abstract

A general caging method for proteins that are regulated by phosphorylation was used to study the in vivo biochemical action of cofilin and the subsequent cellular response. By acute and local activation of a chemically engineered, light-sensitive phosphocofilin mimic, we demonstrate that cofilin polymerizes actin, generates protrusions, and determines the direction of cell migration. We propose a role for cofilin that is distinct from its role as an actin-depolymerizing factor.

Original language	English (US)
Pages (from-to)	743-746
Number of pages	4
Journal	Science
Volume	304
Issue number	5671
DOIs	https://doi.org/10.1126/science.1094561
State	Published - Apr 30 2004

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abstract = "A general caging method for proteins that are regulated by phosphorylation was used to study the in vivo biochemical action of cofilin and the subsequent cellular response. By acute and local activation of a chemically engineered, light-sensitive phosphocofilin mimic, we demonstrate that cofilin polymerizes actin, generates protrusions, and determines the direction of cell migration. We propose a role for cofilin that is distinct from its role as an actin-depolymerizing factor.",

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AU - Ghosh, Mousumi

AU - Song, Xiaoyan

AU - Mouneimne, Ghassan

AU - Sidani, Mazen

AU - Lawrence, David S.

AU - Condeelis, John S.

PY - 2004/4/30

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AB - A general caging method for proteins that are regulated by phosphorylation was used to study the in vivo biochemical action of cofilin and the subsequent cellular response. By acute and local activation of a chemically engineered, light-sensitive phosphocofilin mimic, we demonstrate that cofilin polymerizes actin, generates protrusions, and determines the direction of cell migration. We propose a role for cofilin that is distinct from its role as an actin-depolymerizing factor.

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Cofilin Promotes Actin Polymerization and Defines the Direction of Cell Motility

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