Coenzyme Q10 and lipid-related gene induction in HeLa cells

Constantine Gorelick, Melissa Lopez-Jones, Gary L. Goldberg, Seymour L. Romney, Dineo Khabele

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Objective: Coenzyme Q10 is an antioxidant that may have a therapeutic role in cervical cancer. Study design: We investigated the cellular and molecular effects of 30 μmol/L Coenzyme Q10 in HeLa cells. Cell growth assays, fluorescence-activated cell sorting analyses, and Oil Red O staining were performed. Microarray experiments were performed in duplicate and analyzed on the basis of 2-fold changes in levels of gene expression. Results: Coenzyme Q10 inhibited cell growth and led to apoptosis. Microarray analysis showed that 264 sequences were altered over time, with enrichment in lipid-related genes. Enhanced lipid accumulation was confirmed with Oil Red O staining. Conclusion: A lipid response to Coenzyme Q10 may affect mechanisms of growth inhibition in HeLa cells.

Original languageEnglish (US)
Pages (from-to)1432-1434
Number of pages3
JournalAmerican Journal of Obstetrics and Gynecology
Volume190
Issue number5
DOIs
StatePublished - May 2004

Fingerprint

coenzyme Q10
HeLa Cells
Lipids
Genes
Growth
Staining and Labeling
Microarray Analysis
Uterine Cervical Neoplasms
Flow Cytometry
Antioxidants
Apoptosis
Gene Expression

Keywords

  • Coenzyme Q10
  • Lipid response
  • Microarray

ASJC Scopus subject areas

  • Medicine(all)
  • Obstetrics and Gynecology

Cite this

Gorelick, C., Lopez-Jones, M., Goldberg, G. L., Romney, S. L., & Khabele, D. (2004). Coenzyme Q10 and lipid-related gene induction in HeLa cells. American Journal of Obstetrics and Gynecology, 190(5), 1432-1434. https://doi.org/10.1016/j.ajog.2004.01.076

Coenzyme Q10 and lipid-related gene induction in HeLa cells. / Gorelick, Constantine; Lopez-Jones, Melissa; Goldberg, Gary L.; Romney, Seymour L.; Khabele, Dineo.

In: American Journal of Obstetrics and Gynecology, Vol. 190, No. 5, 05.2004, p. 1432-1434.

Research output: Contribution to journalArticle

Gorelick, C, Lopez-Jones, M, Goldberg, GL, Romney, SL & Khabele, D 2004, 'Coenzyme Q10 and lipid-related gene induction in HeLa cells', American Journal of Obstetrics and Gynecology, vol. 190, no. 5, pp. 1432-1434. https://doi.org/10.1016/j.ajog.2004.01.076
Gorelick, Constantine ; Lopez-Jones, Melissa ; Goldberg, Gary L. ; Romney, Seymour L. ; Khabele, Dineo. / Coenzyme Q10 and lipid-related gene induction in HeLa cells. In: American Journal of Obstetrics and Gynecology. 2004 ; Vol. 190, No. 5. pp. 1432-1434.
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