Coenzyme Q10 and lipid-related gene induction in HeLa cells

Constantine Gorelick; Melissa Lopez-Jones; Gary L. Goldberg; Seymour L. Romney; Dineo Khabele

doi:10.1016/j.ajog.2004.01.076

Coenzyme Q10 and lipid-related gene induction in HeLa cells

Constantine Gorelick, Melissa Lopez-Jones, Gary L. Goldberg, Seymour L. Romney, Dineo Khabele

Obstetrics & Gynecology and Women's Health

Research output: Contribution to journal › Article

13 Scopus citations

Abstract

Objective: Coenzyme Q10 is an antioxidant that may have a therapeutic role in cervical cancer. Study design: We investigated the cellular and molecular effects of 30 μmol/L Coenzyme Q10 in HeLa cells. Cell growth assays, fluorescence-activated cell sorting analyses, and Oil Red O staining were performed. Microarray experiments were performed in duplicate and analyzed on the basis of 2-fold changes in levels of gene expression. Results: Coenzyme Q10 inhibited cell growth and led to apoptosis. Microarray analysis showed that 264 sequences were altered over time, with enrichment in lipid-related genes. Enhanced lipid accumulation was confirmed with Oil Red O staining. Conclusion: A lipid response to Coenzyme Q10 may affect mechanisms of growth inhibition in HeLa cells.

Original language	English (US)
Pages (from-to)	1432-1434
Number of pages	3
Journal	American Journal of Obstetrics and Gynecology
Volume	190
Issue number	5
DOIs	https://doi.org/10.1016/j.ajog.2004.01.076
Publication status	Published - May 2004

Fingerprint

Keywords

Coenzyme Q10
Lipid response
Microarray

ASJC Scopus subject areas

Obstetrics and Gynecology

Cite this

Gorelick, C., Lopez-Jones, M., Goldberg, G. L., Romney, S. L., & Khabele, D. (2004). Coenzyme Q10 and lipid-related gene induction in HeLa cells. American Journal of Obstetrics and Gynecology, 190(5), 1432-1434. https://doi.org/10.1016/j.ajog.2004.01.076

@article{33b39f29053e4e4fa51bf1813b8a99b2,

title = "Coenzyme Q10 and lipid-related gene induction in HeLa cells",

abstract = "Objective: Coenzyme Q10 is an antioxidant that may have a therapeutic role in cervical cancer. Study design: We investigated the cellular and molecular effects of 30 μmol/L Coenzyme Q10 in HeLa cells. Cell growth assays, fluorescence-activated cell sorting analyses, and Oil Red O staining were performed. Microarray experiments were performed in duplicate and analyzed on the basis of 2-fold changes in levels of gene expression. Results: Coenzyme Q10 inhibited cell growth and led to apoptosis. Microarray analysis showed that 264 sequences were altered over time, with enrichment in lipid-related genes. Enhanced lipid accumulation was confirmed with Oil Red O staining. Conclusion: A lipid response to Coenzyme Q10 may affect mechanisms of growth inhibition in HeLa cells.",

keywords = "Coenzyme Q10, Lipid response, Microarray",

author = "Constantine Gorelick and Melissa Lopez-Jones and Goldberg, {Gary L.} and Romney, {Seymour L.} and Dineo Khabele",

year = "2004",

month = "5",

doi = "10.1016/j.ajog.2004.01.076",

language = "English (US)",

volume = "190",

pages = "1432--1434",

journal = "American Journal of Obstetrics and Gynecology",

issn = "0002-9378",

publisher = "Mosby Inc.",

number = "5",

}

TY - JOUR

T1 - Coenzyme Q10 and lipid-related gene induction in HeLa cells

AU - Gorelick, Constantine

AU - Lopez-Jones, Melissa

AU - Goldberg, Gary L.

AU - Romney, Seymour L.

AU - Khabele, Dineo

PY - 2004/5

Y1 - 2004/5

N2 - Objective: Coenzyme Q10 is an antioxidant that may have a therapeutic role in cervical cancer. Study design: We investigated the cellular and molecular effects of 30 μmol/L Coenzyme Q10 in HeLa cells. Cell growth assays, fluorescence-activated cell sorting analyses, and Oil Red O staining were performed. Microarray experiments were performed in duplicate and analyzed on the basis of 2-fold changes in levels of gene expression. Results: Coenzyme Q10 inhibited cell growth and led to apoptosis. Microarray analysis showed that 264 sequences were altered over time, with enrichment in lipid-related genes. Enhanced lipid accumulation was confirmed with Oil Red O staining. Conclusion: A lipid response to Coenzyme Q10 may affect mechanisms of growth inhibition in HeLa cells.

AB - Objective: Coenzyme Q10 is an antioxidant that may have a therapeutic role in cervical cancer. Study design: We investigated the cellular and molecular effects of 30 μmol/L Coenzyme Q10 in HeLa cells. Cell growth assays, fluorescence-activated cell sorting analyses, and Oil Red O staining were performed. Microarray experiments were performed in duplicate and analyzed on the basis of 2-fold changes in levels of gene expression. Results: Coenzyme Q10 inhibited cell growth and led to apoptosis. Microarray analysis showed that 264 sequences were altered over time, with enrichment in lipid-related genes. Enhanced lipid accumulation was confirmed with Oil Red O staining. Conclusion: A lipid response to Coenzyme Q10 may affect mechanisms of growth inhibition in HeLa cells.

KW - Coenzyme Q10

KW - Lipid response

KW - Microarray

UR - http://www.scopus.com/inward/record.url?scp=2542448003&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=2542448003&partnerID=8YFLogxK

U2 - 10.1016/j.ajog.2004.01.076

DO - 10.1016/j.ajog.2004.01.076

M3 - Article

C2 - 15167860

AN - SCOPUS:2542448003

VL - 190

SP - 1432

EP - 1434

JO - American Journal of Obstetrics and Gynecology

JF - American Journal of Obstetrics and Gynecology

SN - 0002-9378

IS - 5

ER -

Access to Document

10.1016/j.ajog.2004.01.076