Coenzyme Q10 and lipid-related gene induction in HeLa cells

Constantine Gorelick; Melissa Lopez-Jones; Gary L. Goldberg; Seymour L. Romney; Dineo Khabele

doi:10.1016/j.ajog.2004.01.076

Coenzyme Q10 and lipid-related gene induction in HeLa cells

Constantine Gorelick, Melissa Lopez-Jones, Gary L. Goldberg, Seymour L. Romney, Dineo Khabele

Research output: Contribution to journal › Article › peer-review

14 Scopus citations

Abstract

Objective: Coenzyme Q10 is an antioxidant that may have a therapeutic role in cervical cancer. Study design: We investigated the cellular and molecular effects of 30 μmol/L Coenzyme Q10 in HeLa cells. Cell growth assays, fluorescence-activated cell sorting analyses, and Oil Red O staining were performed. Microarray experiments were performed in duplicate and analyzed on the basis of 2-fold changes in levels of gene expression. Results: Coenzyme Q10 inhibited cell growth and led to apoptosis. Microarray analysis showed that 264 sequences were altered over time, with enrichment in lipid-related genes. Enhanced lipid accumulation was confirmed with Oil Red O staining. Conclusion: A lipid response to Coenzyme Q10 may affect mechanisms of growth inhibition in HeLa cells.

Original language	English (US)
Pages (from-to)	1432-1434
Number of pages	3
Journal	American journal of obstetrics and gynecology
Volume	190
Issue number	5
DOIs	https://doi.org/10.1016/j.ajog.2004.01.076
State	Published - May 2004
Externally published	Yes

Keywords

Coenzyme Q10
Lipid response
Microarray

ASJC Scopus subject areas

Obstetrics and Gynecology

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.ajog.2004.01.076

Cite this

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title = "Coenzyme Q10 and lipid-related gene induction in HeLa cells",

abstract = "Objective: Coenzyme Q10 is an antioxidant that may have a therapeutic role in cervical cancer. Study design: We investigated the cellular and molecular effects of 30 μmol/L Coenzyme Q10 in HeLa cells. Cell growth assays, fluorescence-activated cell sorting analyses, and Oil Red O staining were performed. Microarray experiments were performed in duplicate and analyzed on the basis of 2-fold changes in levels of gene expression. Results: Coenzyme Q10 inhibited cell growth and led to apoptosis. Microarray analysis showed that 264 sequences were altered over time, with enrichment in lipid-related genes. Enhanced lipid accumulation was confirmed with Oil Red O staining. Conclusion: A lipid response to Coenzyme Q10 may affect mechanisms of growth inhibition in HeLa cells.",

keywords = "Coenzyme Q10, Lipid response, Microarray",

author = "Constantine Gorelick and Melissa Lopez-Jones and Goldberg, {Gary L.} and Romney, {Seymour L.} and Dineo Khabele",

note = "Funding Information: Partially supported by P30-13330 from the National Cancer Institute. ",

year = "2004",

month = may,

doi = "10.1016/j.ajog.2004.01.076",

language = "English (US)",

volume = "190",

pages = "1432--1434",

journal = "American Journal of Obstetrics and Gynecology",

issn = "0002-9378",

publisher = "Mosby Inc.",

number = "5",

}

TY - JOUR

T1 - Coenzyme Q10 and lipid-related gene induction in HeLa cells

AU - Gorelick, Constantine

AU - Lopez-Jones, Melissa

AU - Goldberg, Gary L.

AU - Romney, Seymour L.

AU - Khabele, Dineo

N1 - Funding Information: Partially supported by P30-13330 from the National Cancer Institute.

PY - 2004/5

Y1 - 2004/5

N2 - Objective: Coenzyme Q10 is an antioxidant that may have a therapeutic role in cervical cancer. Study design: We investigated the cellular and molecular effects of 30 μmol/L Coenzyme Q10 in HeLa cells. Cell growth assays, fluorescence-activated cell sorting analyses, and Oil Red O staining were performed. Microarray experiments were performed in duplicate and analyzed on the basis of 2-fold changes in levels of gene expression. Results: Coenzyme Q10 inhibited cell growth and led to apoptosis. Microarray analysis showed that 264 sequences were altered over time, with enrichment in lipid-related genes. Enhanced lipid accumulation was confirmed with Oil Red O staining. Conclusion: A lipid response to Coenzyme Q10 may affect mechanisms of growth inhibition in HeLa cells.

AB - Objective: Coenzyme Q10 is an antioxidant that may have a therapeutic role in cervical cancer. Study design: We investigated the cellular and molecular effects of 30 μmol/L Coenzyme Q10 in HeLa cells. Cell growth assays, fluorescence-activated cell sorting analyses, and Oil Red O staining were performed. Microarray experiments were performed in duplicate and analyzed on the basis of 2-fold changes in levels of gene expression. Results: Coenzyme Q10 inhibited cell growth and led to apoptosis. Microarray analysis showed that 264 sequences were altered over time, with enrichment in lipid-related genes. Enhanced lipid accumulation was confirmed with Oil Red O staining. Conclusion: A lipid response to Coenzyme Q10 may affect mechanisms of growth inhibition in HeLa cells.

KW - Coenzyme Q10

KW - Lipid response

KW - Microarray

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Coenzyme Q10 and lipid-related gene induction in HeLa cells

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

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