Coenzyme Q10 and lipid-related gene induction in HeLa cells

Constantine Gorelick, Melissa Lopez-Jones, Gary L. Goldberg, Seymour L. Romney, Dineo Khabele

Research output: Contribution to journalArticlepeer-review

13 Scopus citations

Abstract

Objective: Coenzyme Q10 is an antioxidant that may have a therapeutic role in cervical cancer. Study design: We investigated the cellular and molecular effects of 30 μmol/L Coenzyme Q10 in HeLa cells. Cell growth assays, fluorescence-activated cell sorting analyses, and Oil Red O staining were performed. Microarray experiments were performed in duplicate and analyzed on the basis of 2-fold changes in levels of gene expression. Results: Coenzyme Q10 inhibited cell growth and led to apoptosis. Microarray analysis showed that 264 sequences were altered over time, with enrichment in lipid-related genes. Enhanced lipid accumulation was confirmed with Oil Red O staining. Conclusion: A lipid response to Coenzyme Q10 may affect mechanisms of growth inhibition in HeLa cells.

Original languageEnglish (US)
Pages (from-to)1432-1434
Number of pages3
JournalAmerican journal of obstetrics and gynecology
Volume190
Issue number5
DOIs
StatePublished - May 2004

Keywords

  • Coenzyme Q10
  • Lipid response
  • Microarray

ASJC Scopus subject areas

  • Obstetrics and Gynecology

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