Abstract
Objective: Coenzyme Q10 is an antioxidant that may have a therapeutic role in cervical cancer. Study design: We investigated the cellular and molecular effects of 30 μmol/L Coenzyme Q10 in HeLa cells. Cell growth assays, fluorescence-activated cell sorting analyses, and Oil Red O staining were performed. Microarray experiments were performed in duplicate and analyzed on the basis of 2-fold changes in levels of gene expression. Results: Coenzyme Q10 inhibited cell growth and led to apoptosis. Microarray analysis showed that 264 sequences were altered over time, with enrichment in lipid-related genes. Enhanced lipid accumulation was confirmed with Oil Red O staining. Conclusion: A lipid response to Coenzyme Q10 may affect mechanisms of growth inhibition in HeLa cells.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 1432-1434 |
| Number of pages | 3 |
| Journal | American Journal of Obstetrics and Gynecology |
| Volume | 190 |
| Issue number | 5 |
| DOIs | |
| State | Published - May 2004 |
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Keywords
- Coenzyme Q10
- Lipid response
- Microarray
ASJC Scopus subject areas
- Medicine(all)
- Obstetrics and Gynecology
Cite this
Coenzyme Q10 and lipid-related gene induction in HeLa cells. / Gorelick, Constantine; Lopez-Jones, Melissa; Goldberg, Gary L.; Romney, Seymour L.; Khabele, Dineo.
In: American Journal of Obstetrics and Gynecology, Vol. 190, No. 5, 05.2004, p. 1432-1434.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Coenzyme Q10 and lipid-related gene induction in HeLa cells
AU - Gorelick, Constantine
AU - Lopez-Jones, Melissa
AU - Goldberg, Gary L.
AU - Romney, Seymour L.
AU - Khabele, Dineo
PY - 2004/5
Y1 - 2004/5
N2 - Objective: Coenzyme Q10 is an antioxidant that may have a therapeutic role in cervical cancer. Study design: We investigated the cellular and molecular effects of 30 μmol/L Coenzyme Q10 in HeLa cells. Cell growth assays, fluorescence-activated cell sorting analyses, and Oil Red O staining were performed. Microarray experiments were performed in duplicate and analyzed on the basis of 2-fold changes in levels of gene expression. Results: Coenzyme Q10 inhibited cell growth and led to apoptosis. Microarray analysis showed that 264 sequences were altered over time, with enrichment in lipid-related genes. Enhanced lipid accumulation was confirmed with Oil Red O staining. Conclusion: A lipid response to Coenzyme Q10 may affect mechanisms of growth inhibition in HeLa cells.
AB - Objective: Coenzyme Q10 is an antioxidant that may have a therapeutic role in cervical cancer. Study design: We investigated the cellular and molecular effects of 30 μmol/L Coenzyme Q10 in HeLa cells. Cell growth assays, fluorescence-activated cell sorting analyses, and Oil Red O staining were performed. Microarray experiments were performed in duplicate and analyzed on the basis of 2-fold changes in levels of gene expression. Results: Coenzyme Q10 inhibited cell growth and led to apoptosis. Microarray analysis showed that 264 sequences were altered over time, with enrichment in lipid-related genes. Enhanced lipid accumulation was confirmed with Oil Red O staining. Conclusion: A lipid response to Coenzyme Q10 may affect mechanisms of growth inhibition in HeLa cells.
KW - Coenzyme Q10
KW - Lipid response
KW - Microarray
UR - http://www.scopus.com/inward/record.url?scp=2542448003&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=2542448003&partnerID=8YFLogxK
U2 - 10.1016/j.ajog.2004.01.076
DO - 10.1016/j.ajog.2004.01.076
M3 - Article
C2 - 15167860
AN - SCOPUS:2542448003
VL - 190
SP - 1432
EP - 1434
JO - American Journal of Obstetrics and Gynecology
JF - American Journal of Obstetrics and Gynecology
SN - 0002-9378
IS - 5
ER -