TY - JOUR
T1 - Codetection of a mixed population of candHPV62 containing wild-type and disrupted E1 open-reading frame in a 45-year-old woman with normal cytology
AU - Fu, Leiping
AU - Terai, Masanori
AU - Matsukura, Toshihiko
AU - Herrero, Rolando
AU - Burk, Robert D.
N1 - Funding Information:
Received 16 January 2004; accepted 2 April 2004; electronically published 18 August 2004. Financial support: National Cancer Institute, National Institutes of Health (grant CA78527 to R.D.B.). a Present affiliation: Department of Reproductive Biology and Pathology, National Research Institute For Child Health and Development, Tokyo, Japan. Reprints or correspondence: Dr. Robert D. Burk, Albert Einstein College of Medicine, Ullmann Bldg., Room 515, 1300 Morris Park Ave., Bronx, NY 10461 (burk@aecom .yu.edu).
PY - 2004/10/1
Y1 - 2004/10/1
N2 - We have cloned, sequenced, and characterized the complete genome of a novel human papillomavirus (HPV), candHPV62. During cloning, 2 candHPV62 viral isolates were recovered from a single cervical sample; 1 had all anticipated HPV open-reading frames (ORFs) intact, whereas the other exhibited an E1 frame-shift mutation. Further experiments indicated that the 2 strains were equivalent in abundance. It appears that an early mutation occurred within the E1 ORF, which was transcomplemented by an intact E1 protein. A search of the HPV database identified disruption of the E1 ORF in the cloned reference isolates of HPV16, HPV53, HPV56, and HPV72. These data suggest that disruption of the E1 ORF in genital HPVs is not uncommon.
AB - We have cloned, sequenced, and characterized the complete genome of a novel human papillomavirus (HPV), candHPV62. During cloning, 2 candHPV62 viral isolates were recovered from a single cervical sample; 1 had all anticipated HPV open-reading frames (ORFs) intact, whereas the other exhibited an E1 frame-shift mutation. Further experiments indicated that the 2 strains were equivalent in abundance. It appears that an early mutation occurred within the E1 ORF, which was transcomplemented by an intact E1 protein. A search of the HPV database identified disruption of the E1 ORF in the cloned reference isolates of HPV16, HPV53, HPV56, and HPV72. These data suggest that disruption of the E1 ORF in genital HPVs is not uncommon.
UR - http://www.scopus.com/inward/record.url?scp=4644247492&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=4644247492&partnerID=8YFLogxK
U2 - 10.1086/423855
DO - 10.1086/423855
M3 - Article
C2 - 15346342
AN - SCOPUS:4644247492
SN - 0022-1899
VL - 190
SP - 1303
EP - 1309
JO - Journal of Infectious Diseases
JF - Journal of Infectious Diseases
IS - 7
ER -
