Codetection of a mixed population of candHPV62 containing wild-type and disrupted E1 open-reading frame in a 45-year-old woman with normal cytology

Leiping Fu, Masanori Terai, Toshihiko Matsukura, Rolando Herrero, Robert D. Burk

Research output: Contribution to journalArticle

5 Scopus citations

Abstract

We have cloned, sequenced, and characterized the complete genome of a novel human papillomavirus (HPV), candHPV62. During cloning, 2 candHPV62 viral isolates were recovered from a single cervical sample; 1 had all anticipated HPV open-reading frames (ORFs) intact, whereas the other exhibited an E1 frame-shift mutation. Further experiments indicated that the 2 strains were equivalent in abundance. It appears that an early mutation occurred within the E1 ORF, which was transcomplemented by an intact E1 protein. A search of the HPV database identified disruption of the E1 ORF in the cloned reference isolates of HPV16, HPV53, HPV56, and HPV72. These data suggest that disruption of the E1 ORF in genital HPVs is not uncommon.

Original languageEnglish (US)
Pages (from-to)1303-1309
Number of pages7
JournalJournal of Infectious Diseases
Volume190
Issue number7
DOIs
StatePublished - Oct 1 2004

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ASJC Scopus subject areas

  • Immunology and Allergy
  • Infectious Diseases

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