Codeletion of p15 and p16 in primary malignant mesothelioma

S. Xiao, D. Li, Jan Vijg, D. J. Sugarbaker, J. M. Corson, J. A. Fletcher

Research output: Contribution to journalArticle

146 Citations (Scopus)

Abstract

The p15 and p16 CDK4 inhibitor genes map within the chromosome band 9p21 region deleted frequently in malignant mesothelioma and other cancers. p16 has been implicated recently as a potential target of 9p21 deletions in mesothelioma, but the role of this gene is uncertain because deletions have been detected more often in established cell lines than in primary tumor specimens. We determined p15 and p16 copy number by fluorescence in situ hybridization with a P1 contig in 50 primary mesotheliomas. Codeletion of p15 and p16 was found in 72% of mesotheliomas, including all cases with spindle-cell components (n = 21) and total deletion of p15 and p16 was found in several mesotheliomas that lacked cytogenetic deletion of the chromosome 9 short arm. Point mutations were not found, however, in exon 2 of retained p15 and p16 alleles from seven mesotheliomas. These findings demonstrate that p15, p16 and/or a closely neighboring gene, are the targets of frequent chromosome 9p deletion in primary malignant mesothelioma.

Original languageEnglish (US)
Pages (from-to)511-515
Number of pages5
JournalOncogene
Volume11
Issue number3
StatePublished - 1995
Externally publishedYes

Fingerprint

Mesothelioma
Cyclin-Dependent Kinase Inhibitor p15
Genes
Chromosome Deletion
Chromosomes, Human, Pair 9
Cellular Structures
Fluorescence In Situ Hybridization
Point Mutation
Cytogenetics
Exons
Neoplasms
Chromosomes
Alleles
Malignant Mesothelioma
Cell Line

Keywords

  • Cyclin-dependent kinases
  • Cyclins
  • In situ hybridization
  • Mesothelioma

ASJC Scopus subject areas

  • Cancer Research
  • Genetics
  • Molecular Biology

Cite this

Xiao, S., Li, D., Vijg, J., Sugarbaker, D. J., Corson, J. M., & Fletcher, J. A. (1995). Codeletion of p15 and p16 in primary malignant mesothelioma. Oncogene, 11(3), 511-515.

Codeletion of p15 and p16 in primary malignant mesothelioma. / Xiao, S.; Li, D.; Vijg, Jan; Sugarbaker, D. J.; Corson, J. M.; Fletcher, J. A.

In: Oncogene, Vol. 11, No. 3, 1995, p. 511-515.

Research output: Contribution to journalArticle

Xiao, S, Li, D, Vijg, J, Sugarbaker, DJ, Corson, JM & Fletcher, JA 1995, 'Codeletion of p15 and p16 in primary malignant mesothelioma', Oncogene, vol. 11, no. 3, pp. 511-515.
Xiao S, Li D, Vijg J, Sugarbaker DJ, Corson JM, Fletcher JA. Codeletion of p15 and p16 in primary malignant mesothelioma. Oncogene. 1995;11(3):511-515.
Xiao, S. ; Li, D. ; Vijg, Jan ; Sugarbaker, D. J. ; Corson, J. M. ; Fletcher, J. A. / Codeletion of p15 and p16 in primary malignant mesothelioma. In: Oncogene. 1995 ; Vol. 11, No. 3. pp. 511-515.
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